High-throughput sorting of an Anticalin library via EspP-mediated functional display on the Escherichia coli cell surface.
about
Crystal Structure of the Passenger Domain of the Escherichia coli Autotransporter EspPA nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacityLooks can be deceiving: recent insights into the mechanism of protein secretion by the autotransporter pathway.Anticalins: exploiting a non-Ig scaffold with hypervariable loops for the engineering of binding proteins.Isolation of a potential anchoring motif based on proteome analysis of Escherichia coli and its use for cell surface display.Single-cell characterization of autotransporter-mediated Escherichia coli surface display of disulfide bond-containing proteins.Bacterial display in combinatorial protein engineering.Autotransporter-based cell surface display in Gram-negative bacteria.Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies.An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains.Selection and characterization of Anticalins targeting human prostate-specific membrane antigen (PSMA).Antibody affinity maturation through combining display of two-chain paired antibody and precision flow cytometric sorting.Autotransporters with GDSL passenger domains: molecular physiology and biotechnological applications.Co-expression of active human cytochrome P450 1A2 and cytochrome P450 reductase on the cell surface of Escherichia coli.Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities.Enhanced rhamnolipid production by Pseudomonas aeruginosa overexpressing estA in a simple medium.Probing the applicability of autotransporter based surface display with the EstA autotransporter of Pseudomonas stutzeri A15.Fusion with the cold-active esterase facilitates autotransporter-based surface display of the 10th human fibronectin domain in Escherichia coli.Manganese and cobalt recovery by surface display of metal binding peptide on various loops of OmpC in Escherichia coli.Redesigning of Microbial Cell Surface and Its Application to Whole-Cell Biocatalysis and Biosensors.Autodisplay of glucose-6-phosphate dehydrogenase for redox cofactor regeneration at the cell surface.Anticalin® Proteins as Therapeutic Agents in Human Diseases.
P2860
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P2860
High-throughput sorting of an Anticalin library via EspP-mediated functional display on the Escherichia coli cell surface.
description
2010 nî lūn-bûn
@nan
2010 թուականի Մայիսին հրատարակուած գիտական յօդուած
@hyw
2010 թվականի մայիսին հրատարակված գիտական հոդված
@hy
2010年の論文
@ja
2010年論文
@yue
2010年論文
@zh-hant
2010年論文
@zh-hk
2010年論文
@zh-mo
2010年論文
@zh-tw
2010年论文
@wuu
name
High-throughput sorting of an ...... Escherichia coli cell surface.
@ast
High-throughput sorting of an ...... Escherichia coli cell surface.
@en
type
label
High-throughput sorting of an ...... Escherichia coli cell surface.
@ast
High-throughput sorting of an ...... Escherichia coli cell surface.
@en
prefLabel
High-throughput sorting of an ...... Escherichia coli cell surface.
@ast
High-throughput sorting of an ...... Escherichia coli cell surface.
@en
P2093
P1476
High-throughput sorting of an ...... Escherichia coli cell surface.
@en
P2093
Gabriele Matschiner
Ina Theobald
Uli Binder
P304
P356
10.1016/J.JMB.2010.05.049
P50
P577
2010-05-26T00:00:00Z