Intein applications: from protein purification and labeling to metabolic control methods.
about
Hedgehog Cholesterolysis: Specialized Gatekeeper to Oncogenic SignalingAn Engineered Split Intein for Photoactivated Protein Trans-SplicingChemical and biological tools for the preparation of modified histone proteinsEvolution, mechanisms, and applications of intein-mediated protein splicing.Protein splicing: how inteins escape from precursor proteins.Dynamics differentiate between active and inactive inteins.Förster resonance energy transfer-based cholesterolysis assay identifies a novel hedgehog inhibitor.A Streamlined, Automated Protocol for the Production of Milligram Quantities of Untagged Recombinant Rat Lactate Dehydrogenase A Using ÄKTAxpressTM.Chemical tagging and customizing of cellular chromatin states using ultrafast trans-splicing inteinsNanobody-based cancer therapy of solid tumors.A cleavable self-assembling tag strategy for preparing proteins and peptides with an authentic N-terminus.Modular protein domains: an engineering approach toward functional biomaterials.Elastin-like polypeptides: A strategic fusion partner for biologics.Purification of Antibacterial CHAPK Protein Using a Self-Cleaving Fusion Tag and Its Activity Against Methicillin-Resistant Staphylococcus aureus.A split intein T7 RNA polymerase for transcriptional AND-logic.Backbone circularization of Bacillus subtilis family 11 xylanase increases its thermostability and its resistance against aggregation.Sequential formation of two branched intermediates during protein splicing of class three inteinsA molecular engineering toolbox for the structural biologist.Conditional Protein Splicing Switch in Hyperthermophiles through an Intein-Extein Partnership.A novel self-cleavable tag Zbasic-∆I-CM and its application in the soluble expression of recombinant human interleukin-15 in Escherichia coli.Semisynthesis of Membrane-Attached Proteins Using Split Inteins.Split Ssp DnaB mini-intein-mediated production of recombinant human glucagon-like peptide-1/7-36.A Cassette Approach for the Identification of Intein Insertion Sites.Butelase-mediated cyclization and ligation of peptides and proteins.Segmental Isotopic Labeling of Proteins for NMR Study Using Intein Technology.Development of a disaggregation-induced emission probe for the detection of RecA inteins from Mycobacterium tuberculosis.An efficient protocol towards site-specifically clickable nanobodies in high yield: cytoplasmic expression in Escherichia coli combined with intein-mediated protein ligation.Inteins: Localized Distribution, Gene Regulation, and Protein Engineering for Biological Applications.
P2860
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P2860
Intein applications: from protein purification and labeling to metabolic control methods.
description
2014 nî lūn-bûn
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2014 թուականի Ապրիլին հրատարակուած գիտական յօդուած
@hyw
2014 թվականի ապրիլին հրատարակված գիտական հոդված
@hy
2014年の論文
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2014年論文
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2014年論文
@zh-hant
2014年論文
@zh-hk
2014年論文
@zh-mo
2014年論文
@zh-tw
2014年论文
@wuu
name
Intein applications: from protein purification and labeling to metabolic control methods.
@ast
Intein applications: from protein purification and labeling to metabolic control methods.
@en
type
label
Intein applications: from protein purification and labeling to metabolic control methods.
@ast
Intein applications: from protein purification and labeling to metabolic control methods.
@en
prefLabel
Intein applications: from protein purification and labeling to metabolic control methods.
@ast
Intein applications: from protein purification and labeling to metabolic control methods.
@en
P2860
P356
P1476
Intein applications: from protein purification and labeling to metabolic control methods.
@en
P2093
David W Wood
Julio A Camarero
P2860
P304
14512-14519
P356
10.1074/JBC.R114.552653
P407
P577
2014-04-02T00:00:00Z