Direct DNA amplification from crude clinical samples using a PCR enhancer cocktail and novel mutants of Taq
about
Digital quantification of miRNA directly in plasma using integrated comprehensive droplet digital detection.1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancerTransformation of personal computers and mobile phones into genetic diagnostic systemsA direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification.Evaluation of inhibitor-resistant real-time PCR methods for diagnostics in clinical and environmental samples.Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination.PCR amplification of a triple-repeat genetic target directly from whole blood in 15 minutes as a proof-of-principle PCR study for direct sample analysis for a clinically relevant targetProperties of targeted preamplification in DNA and cDNA quantification.Detection of Mycobacterium tuberculosis in blood by use of the Xpert MTB/RIF assay.Point-of-care nucleic acid testing for infectious diseases.Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read (>11 kb), single molecule, real-time sequencing.Direct cell lysis for single-cell gene expression profilingReal-time PCR detection of Plasmodium directly from whole blood and filter paper samples.Colony PCR.Modified DNA polymerases for PCR troubleshootingMetal nanoparticle assisted polymerase chain reaction for strain typing of Salmonella Typhi.A fast and accurate method of detecting Aleutian mink disease virus in blood and tissues of chronically infected mink.Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance.A novel mechanism for direct real-time polymerase chain reaction that does not require DNA isolation from prokaryotic cells.The remaining challenges to laboratory-based surveillance of invasive pneumococcal disease.Field-deployable, quantitative, rapid identification of active Ebola virus infection in unprocessed blood.Forensic animal DNA analysis using economical two-step direct PCR.Sample-to-answer acoustic detection of DNA in complex samples.Novel Development of a qPCR Assay Based on the rpoB Gene for Rapid Detection of Cronobacter spp.Cost-effective optimization of real-time PCR-based detection of Campylobacter and Salmonella with inhibitor tolerant DNA polymerases.Direct DNA and RNA detection from large volumes of whole human blood.Most probable number - loop mediated isothermal amplification (MPN-LAMP) for quantifying waterborne pathogens in <25min.Inhibition mechanisms of hemoglobin, immunoglobulin G, and whole blood in digital and real-time PCR.Competitive allele-specific hairpin primer PCR for extremely high allele discrimination in typing of single nucleotide polymorphisms.A Universal Fast Colorimetric Method for DNA Signal Detection with DNA Strand Displacement and Gold Nanoparticles
P2860
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P2860
Direct DNA amplification from crude clinical samples using a PCR enhancer cocktail and novel mutants of Taq
description
2010 nî lūn-bûn
@nan
2010 թուականի Յունուարին հրատարակուած գիտական յօդուած
@hyw
2010 թվականի հունվարին հրատարակված գիտական հոդված
@hy
2010年の論文
@ja
2010年論文
@yue
2010年論文
@zh-hant
2010年論文
@zh-hk
2010年論文
@zh-mo
2010年論文
@zh-tw
2010年论文
@wuu
name
Direct DNA amplification from ...... ktail and novel mutants of Taq
@ast
Direct DNA amplification from ...... ktail and novel mutants of Taq
@en
type
label
Direct DNA amplification from ...... ktail and novel mutants of Taq
@ast
Direct DNA amplification from ...... ktail and novel mutants of Taq
@en
prefLabel
Direct DNA amplification from ...... ktail and novel mutants of Taq
@ast
Direct DNA amplification from ...... ktail and novel mutants of Taq
@en
P2093
P2860
P1476
Direct DNA amplification from ...... ktail and novel mutants of Taq
@en
P2093
Milko B Kermekchiev
Wayne M Barnes
Zhian Zhang
P2860
P304
P356
10.2353/JMOLDX.2010.090070
P577
2010-01-14T00:00:00Z