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Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila

Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila

http://www.wikidata.org/entity/Q34605914

about

A new age in functional genomics using CRISPR/Cas9 in arrayed library screening Off-target Effects in CRISPR/Cas9-mediated Genome Engineering Selective gene dosage by CRISPR-Cas9 genome editing in hexaploid Camelina sativa.Cloning-free CRISPR Effective knockdown of Drosophila long non-coding RNAs by CRISPR interference.Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design.Genome engineering with CRISPR-Cas9 in the mosquito Aedes aegypti CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells.A genome-wide CRISPR library for high-throughput genetic screening in Drosophila cells.Systematic Evaluation of Drosophila CRISPR Tools Reveals Safe and Robust Alternatives to Autonomous Gene Drives in Basic Research.Genome Editing with CRISPR-Cas9: Can It Get Any Better?Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR.CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo Resistance mutation conserved between insects and mites unravels the benzoylurea insecticide mode of action on chitin biosynthesis.Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi.Functional interrogation of non-coding DNA through CRISPR genome editing.Examination of CRISPR/Cas9 design tools and the effect of target site accessibility on Cas9 activity.Genome engineering: Drosophila melanogaster and beyond CRISPR/cas9, a novel genomic tool to knock down microRNA in vitro and in vivo.CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering.CT-Finder: A Web Service for CRISPR Optimal Target Prediction and Visualization.Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9.Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Coconversion.Profiling single-guide RNA specificity reveals a mismatch sensitive core sequence.SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae.Recent Advances in CRISPR-Cas9 Genome Editing Technology for Biological and Biomedical Investigations.A quick guide to CRISPR sgRNA design tools.Precise and efficient scarless genome editing in stem cells using CORRECT.Genome editing in Drosophila melanogaster: from basic genome engineering to the multipurpose CRISPR-Cas9 system.Nuclear domain 'knock-in' screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing.The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models CRISPR-Cas9 technology: applications and human disease modelling.A Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.Gene Drive for Mosquito Control: Where Did It Come from and Where Are We Headed?Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System.Progress and Prospects of CRISPR/Cas Systems in Insects and Other Arthropods.Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system.Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9.Temperature-dependent sex-reversal by a transformer-2 gene-edited mutation in the spotted wing drosophila, Drosophila suzukii

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Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila

http://www.wikidata.org/entity/Q34605914

description

2014 nî lūn-bûn

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2014 թուականի Հոկտեմբերին հրատարակուած գիտական յօդուած

@hyw

2014 թվականի հոտեմբերին հրատարակված գիտական հոդված

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2014年の論文

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2014年論文

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2014年論文

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2014年論文

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2014年論文

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2014年論文

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2014年论文

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Enhanced specificity and effic ...... sgRNA parameters in Drosophila

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Enhanced specificity and effic ...... sgRNA parameters in Drosophila

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Enhanced specificity and effic ...... sgRNA parameters in Drosophila

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Enhanced specificity and effic ...... sgRNA parameters in Drosophila

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Enhanced specificity and effic ...... sgRNA parameters in Drosophila

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Enhanced specificity and effic ...... sgRNA parameters in Drosophila

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Enhanced specificity and effic ...... sgRNA parameters in Drosophila

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Decai Mao

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Huan-Huan Qiao

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Jian-Quan Ni

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Jiang Xu

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Jin Billy Li

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Jin Sun

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Jun-Yuan Ji

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Lu-Ping Liu

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Patricia Deng

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Qun Hu

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P2860

RNA-guided human genome engineering via Cas9

Heritable genome editing in C. elegans via a CRISPR-Cas9 system.

Multiplex genome engineering using CRISPR/Cas systems

Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

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