Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing.
about
Two-round coamplification at lower denaturation temperature-PCR (COLD-PCR)-based sanger sequencing identifies a novel spectrum of low-level mutations in lung adenocarcinomaPCR-based methods for the enrichment of minority alleles and mutationsCirculating DNA in diagnosis and monitoring EGFR gene mutations in advanced non-small cell lung cancerMutation status concordance between primary lesions and metastatic sites of advanced non-small-cell lung cancer and the impact of mutation testing methodologies: a literature reviewTargeting tyrosine kinases in cancer: the converging roles of cytopathology and molecular pathology in the era of genomic medicineSomatic mosaicism: implications for disease and transmission geneticsQuantitative and sensitive detection of rare mutations using droplet-based microfluidicsDNA suspension arrays: silencing discrete artifacts for high-sensitivity applicationsModified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked PrimerDetection and quantification of rare mutations with massively parallel sequencing.A Phase II Trial of AZD6244 (Selumetinib, ARRY-142886), an Oral MEK1/2 Inhibitor, in Relapsed/Refractory Multiple MyelomaMethylation-sensitive enrichment of minor DNA alleles using a double-strand DNA-specific nuclease.Expanded genetic codes in next generation sequencing enable decontamination and mitochondrial enrichment.Genetic Polymorphism in Wine Yeasts: Mechanisms and Methods for Its DetectionCOLD-PCR-enhanced high-resolution melting enables rapid and selective identification of low-level unknown mutationsA branch-migration based fluorescent probe for straightforward, sensitive and specific discrimination of DNA mutations.COLD-PCR enhanced melting curve analysis improves diagnostic accuracy for KRAS mutations in colorectal carcinoma.Mutation scanning of peach floral genes.Reappraisal of the so-called 'villous tumours' of the rectosigmoid, based on histological, immunohistochemical and genotypic features.Using genetics and genomics strategies to personalize therapy for cancer: focus on melanomaKinetic hairpin oligonucleotide blockers for selective amplification of rare mutations.Screening for EGFR and KRAS mutations in endobronchial ultrasound derived transbronchial needle aspirates in non-small cell lung cancer using COLD-PCR.Detection of hepatitis B virus genotypic resistance mutations by coamplification at lower denaturation temperature-PCR coupled with sanger sequencing.The use of COLD-PCR and high-resolution melting analysis improves the limit of detection of KRAS and BRAF mutations in colorectal cancer.COLD-PCR enrichment of rare cancer mutations prior to targeted amplicon resequencing.A comparison of EGFR mutation testing methods in lung carcinoma: direct sequencing, real-time PCR and immunohistochemistryIce-COLD-PCR enables rapid amplification and robust enrichment for low-abundance unknown DNA mutations.Competitive amplification of differentially melting amplicons (CADMA) improves KRAS hotspot mutation testing in colorectal cancerKRAS and BRAF mutation status in circulating colorectal tumor cells and their correlation with primary and metastatic tumor tissue.Non-invasive prenatal diagnosis of multiple endocrine neoplasia type 2A using COLD-PCR combined with HRM genotyping analysis from maternal serumEnrichment of mutations in multiple DNA sequences using COLD-PCR in emulsion.Depletion of Abundant Sequences by Hybridization (DASH): using Cas9 to remove unwanted high-abundance species in sequencing libraries and molecular counting applications.Detection of low-abundance KRAS mutations in colorectal cancer using microfluidic capillary electrophoresis-based restriction fragment length polymorphism method with optimized assay conditionsFull COLD-PCR protocol for noninvasive prenatal diagnosis of genetic diseasesNRAS mutations with low allele burden have independent prognostic significance for patients with lower risk myelodysplastic syndromes.Genetic diversity of Borrelia burgdorferi and detection of B. bissettii-like DNA in serum of north-coastal California residents.Single-tube, highly parallel mutation enrichment in cancer gene panels by use of temperature-tolerant COLD-PCR.Application of COLD-PCR for improved detection of KRAS mutations in clinical samples.Suitability of surgical tumor tissues, biopsy, or cytology samples for epidermal growth factor receptor mutation testing in non-small cell lung carcinoma based on chinese population.COLD-PCR: improving the sensitivity of molecular diagnostics assays.
P2860
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P2860
Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing.
description
2008 nî lūn-bûn
@nan
2008 թուականի Ապրիլին հրատարակուած գիտական յօդուած
@hyw
2008 թվականի ապրիլին հրատարակված գիտական հոդված
@hy
2008年の論文
@ja
2008年論文
@yue
2008年論文
@zh-hant
2008年論文
@zh-hk
2008年論文
@zh-mo
2008年論文
@zh-tw
2008年论文
@wuu
name
Replacing PCR with COLD-PCR en ...... ensitivity of genetic testing.
@ast
Replacing PCR with COLD-PCR en ...... ensitivity of genetic testing.
@en
Replacing PCR with COLD-PCR en ...... ensitivity of genetic testing.
@nl
type
label
Replacing PCR with COLD-PCR en ...... ensitivity of genetic testing.
@ast
Replacing PCR with COLD-PCR en ...... ensitivity of genetic testing.
@en
Replacing PCR with COLD-PCR en ...... ensitivity of genetic testing.
@nl
prefLabel
Replacing PCR with COLD-PCR en ...... ensitivity of genetic testing.
@ast
Replacing PCR with COLD-PCR en ...... ensitivity of genetic testing.
@en
Replacing PCR with COLD-PCR en ...... ensitivity of genetic testing.
@nl
P2093
P356
P1433
P1476
Replacing PCR with COLD-PCR en ...... ensitivity of genetic testing.
@en
P2093
G Mike Makrigiorgos
Harvey Mamon
Lilin Wang
Matthew H Kulke
Ross Berbeco
P2888
P304
P356
10.1038/NM1708
P407
P577
2008-04-13T00:00:00Z