Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids. - Wikidata - wikimedia - TriplyDB

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

http://www.wikidata.org/entity/Q35062969

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Constitutive internalization of the leucine-rich G protein-coupled receptor-5 (LGR5) to the trans-Golgi network Identifying the Role of E2 Domains on Alphavirus Neutralization and Protective Immune Responses Dissecting the Role of E2 Protein Domains in Alphavirus Pathogenicity Light-mediated kinetic control reveals the temporal effect of the Raf/MEK/ERK pathway in PC12 cell neurite outgrowth Optogenetic control of molecular motors and organelle distributions in cells.Chaperone-mediated native folding of a β-scorpion toxin in the periplasm of Escherichia coli High-throughput recombinant protein expression in Escherichia coli: current status and future perspectives New Trends of Digital Data Storage in DNA Molecular characterization of a glycerol-3-phosphate acyltransferase reveals key features essential for triacylglycerol production in Phaeodactylum tricornutum A Rewritable, Random-Access DNA-Based Storage System Local and global consequences of flow on bacterial quorum sensing Recent advances in universal TA cloning methods for use in function studies.FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method Bovine serum albumin further enhances the effects of organic solvents on increased yield of polymerase chain reaction of GC-rich templates.Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria Construction of a synthetic metabolic pathway for biosynthesis of the non-natural methionine precursor 2,4-dihydroxybutyric acid.Meru couples planar cell polarity with apical-basal polarity during asymmetric cell division Construction and evaluation of a novel humanized HER2-specific chimeric receptor Streptococcus pyogenes polymyxin B-resistant mutants display enhanced ExPortal integrity.Elucidation of the cryptic epimerase activity of redox-inactive ketoreductase domains from modular polyketide synthases by tandem equilibrium isotope exchange The eukaryotic signal sequence, YGRL, targets the chlamydial inclusion.Inverse fusion PCR cloning.Severe alpha-1 antitrypsin deficiency in composite heterozygotes inheriting a new splicing mutation QOMadrid An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy.Identification and characterization of an operon, msaABCR, that controls virulence and biofilm development in Staphylococcus aureus.Transformable facultative thermophile Geobacillus stearothermophilus NUB3621 as a host strain for metabolic engineering.Recent advances in DNA assembly technologies White and green screening with circular polymerase extension cloning for easy and reliable cloning Digestion of Nucleic Acids Starts in the Stomach.Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry.The use of amino sugars by Bacillus subtilis: presence of a unique operon for the catabolism of glucosamine.SOMA: a single oligonucleotide mutagenesis and cloning approach One primer to rule them all: universal primer that adds BBa_B0034 ribosomal binding site to any coding standard 10 BioBrick Combinatorial library of improved peptide aptamers, CLIPs to inhibit RAGE signal transduction in mammalian cells.A cleavable N-terminal signal peptide promotes widespread olfactory receptor surface expression in HEK293T cells.The essential role of the Deinococcus radiodurans ssb gene in cell survival and radiation tolerance.Estrogen receptor-alpha 36 mediates the anti-apoptotic effect of estradiol in triple negative breast cancer cells via a membrane-associated mechanism.Directed evolution of aminoglycoside phosphotransferase (3') type IIIa variants that inactivate amikacin but impose significant fitness costs.A practical comparison of ligation-independent cloning techniques Escherichia coli deletion mutants illuminate trade-offs between growth rate and flux through a foreign anabolic pathway.

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P2860

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

http://www.wikidata.org/entity/Q35062969

description

2010 nî lūn-bûn

@nan

2010 թուականի Յունիսին հրատարակուած գիտական յօդուած

@hyw

2010 թվականի հունիսին հրատարակված գիտական հոդված

@hy

2010年の論文

@ja

2010年論文

@yue

2010年論文

@zh-hant

2010年論文

@zh-hk

2010年論文

@zh-mo

2010年論文

@zh-tw

2010年论文

@wuu

name

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

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Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

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type

label

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

@ast

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

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prefLabel

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

@ast

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

@en

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P1476

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

@en

P2093

Anton V Bryksin

http://www.w3.org/2001/XMLSchema#string

Ichiro Matsumura

http://www.w3.org/2001/XMLSchema#string

P2860

Genetic engineering using homologous recombination.

PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products.

One-step DNA fragment assembly and circularization for gene cloning.

Concerted assembly and cloning of multiple DNA segments using in vitro site-specific recombination: functional analysis of multi-segment expression clones.

Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.

Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously

A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro

Ligation independent cloning vectors for expression of SUMO fusions.

GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes.

Ligase-free subcloning: a versatile method to subclone polymerase chain reaction (PCR) products in a single day.

P304

463-465

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scholarly article

P356

10.2144/000113418

http://www.w3.org/2001/XMLSchema#string

P433

6

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P478

48

http://www.w3.org/2001/XMLSchema#string

P577

2010-06-01T00:00:00Z

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44693649

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P698

20569222

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P932

3121328

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