Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.
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Constitutive internalization of the leucine-rich G protein-coupled receptor-5 (LGR5) to the trans-Golgi networkIdentifying the Role of E2 Domains on Alphavirus Neutralization and Protective Immune ResponsesDissecting the Role of E2 Protein Domains in Alphavirus PathogenicityLight-mediated kinetic control reveals the temporal effect of the Raf/MEK/ERK pathway in PC12 cell neurite outgrowthOptogenetic control of molecular motors and organelle distributions in cells.Chaperone-mediated native folding of a β-scorpion toxin in the periplasm of Escherichia coliHigh-throughput recombinant protein expression in Escherichia coli: current status and future perspectivesNew Trends of Digital Data Storage in DNAMolecular characterization of a glycerol-3-phosphate acyltransferase reveals key features essential for triacylglycerol production in Phaeodactylum tricornutumA Rewritable, Random-Access DNA-Based Storage SystemLocal and global consequences of flow on bacterial quorum sensingRecent advances in universal TA cloning methods for use in function studies.FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning methodBovine serum albumin further enhances the effects of organic solvents on increased yield of polymerase chain reaction of GC-rich templates.Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteriaConstruction of a synthetic metabolic pathway for biosynthesis of the non-natural methionine precursor 2,4-dihydroxybutyric acid.Meru couples planar cell polarity with apical-basal polarity during asymmetric cell divisionConstruction and evaluation of a novel humanized HER2-specific chimeric receptorStreptococcus pyogenes polymyxin B-resistant mutants display enhanced ExPortal integrity.Elucidation of the cryptic epimerase activity of redox-inactive ketoreductase domains from modular polyketide synthases by tandem equilibrium isotope exchangeThe eukaryotic signal sequence, YGRL, targets the chlamydial inclusion.Inverse fusion PCR cloning.Severe alpha-1 antitrypsin deficiency in composite heterozygotes inheriting a new splicing mutation QOMadridAn asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy.Identification and characterization of an operon, msaABCR, that controls virulence and biofilm development in Staphylococcus aureus.Transformable facultative thermophile Geobacillus stearothermophilus NUB3621 as a host strain for metabolic engineering.Recent advances in DNA assembly technologiesWhite and green screening with circular polymerase extension cloning for easy and reliable cloningDigestion of Nucleic Acids Starts in the Stomach.Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry.The use of amino sugars by Bacillus subtilis: presence of a unique operon for the catabolism of glucosamine.SOMA: a single oligonucleotide mutagenesis and cloning approachOne primer to rule them all: universal primer that adds BBa_B0034 ribosomal binding site to any coding standard 10 BioBrickCombinatorial library of improved peptide aptamers, CLIPs to inhibit RAGE signal transduction in mammalian cells.A cleavable N-terminal signal peptide promotes widespread olfactory receptor surface expression in HEK293T cells.The essential role of the Deinococcus radiodurans ssb gene in cell survival and radiation tolerance.Estrogen receptor-alpha 36 mediates the anti-apoptotic effect of estradiol in triple negative breast cancer cells via a membrane-associated mechanism.Directed evolution of aminoglycoside phosphotransferase (3') type IIIa variants that inactivate amikacin but impose significant fitness costs.A practical comparison of ligation-independent cloning techniquesEscherichia coli deletion mutants illuminate trade-offs between growth rate and flux through a foreign anabolic pathway.
P2860
Q24320381-2009C8BE-8A62-4BF5-A9BD-852FC6B63296Q24701741-7475DE99-2534-43A6-8E46-9388F8CFB2A1Q26314381-72269AB9-A044-46A6-822C-F4D4F2D50003Q27313024-C82A9218-EA59-4D80-9AB1-8C178D1AE784Q27318638-193C7D97-6F81-4EEB-A52D-88F48DFD113AQ27679850-6E13CC38-D7EF-4550-B719-A23380A85C31Q28066811-222E05D9-040D-4061-902A-9AC2D1988397Q28072505-C9017C0E-9B72-4067-8992-C99058312BD8Q28602766-AA18230F-E949-42BC-B620-8776D82ACC16Q28607191-E6A74616-097A-4809-BC73-BF06DDA5FAF9Q28831427-1FA6947B-FC8B-4895-A0DF-2CD9ED15A425Q30392430-FFB2219C-9128-45BB-80A1-8F2DF8AFCF80Q31033460-5D2BA537-46C1-4801-B3A4-835DDCA42782Q31062366-7B288959-0ED9-41F0-82C8-73E22F66F926Q33719322-F7ABC984-A2CF-4A3C-8ADE-02DDAAC694B3Q33827115-343465A4-2038-4073-BFF9-A3E881F5CCFFQ33860435-B2F91CC9-EE1D-4283-8706-8CF053FD0881Q33892746-85309EE1-4EDB-4A86-995B-7740C4D915D1Q33899541-C36D5B05-E0BA-4493-96BB-014299506797Q33951190-77D8A539-6997-47DA-95EE-24DE87D7A071Q34164934-EF82E3AE-1AC8-4F89-AFEA-E04653A41599Q34243738-1202EF74-6BFC-4044-A5FD-EAD1B5E8CBCFQ34325994-EAFB755D-2633-4E19-841B-3EE29A6DD6AAQ34329333-10A3E63B-325E-4AF3-91DF-8DD9D9D76C73Q34500640-D3104852-6F18-463F-A9F3-BC48FF8AC97AQ34610863-E00BABF7-8D22-4930-B897-0F4A7691FF2BQ34642239-0155B768-A989-4981-A008-7D2D1668FB74Q34671573-14CD5258-6B63-4F08-9A71-2B943C9E735AQ34671611-DC744634-FCE9-431F-AA8F-5BBD8C97FB12Q34698762-43D8E626-330D-45F3-B688-2E41DD917120Q34717964-1FFE76A0-DCBC-4A2B-9B32-10170335FF8FQ34764129-45FBD164-E416-4727-BDC5-097395E3EF34Q34778257-E98E1FF4-F5ED-420F-8B3E-F82A6B9CB64CQ34778644-6163C5F5-E45A-4946-9286-48CC564D3266Q34809247-42CAA970-18D0-4BBF-997A-5A63BBFCB320Q34950190-7BE18806-5862-4A62-9112-E312E7A9F7B4Q34976544-1AA83D16-6D1F-4243-B3E3-A865454DCDFDQ35034759-C9976443-7E78-464B-B9D8-4F02EE7375EDQ35078266-DCC21BEB-4C8E-4BE3-90AC-347853D91BA7Q35088539-29C3896A-426F-4855-8902-99995F1535CE
P2860
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.
description
2010 nî lūn-bûn
@nan
2010 թուականի Յունիսին հրատարակուած գիտական յօդուած
@hyw
2010 թվականի հունիսին հրատարակված գիտական հոդված
@hy
2010年の論文
@ja
2010年論文
@yue
2010年論文
@zh-hant
2010年論文
@zh-hk
2010年論文
@zh-mo
2010年論文
@zh-tw
2010年论文
@wuu
name
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.
@ast
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.
@en
type
label
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.
@ast
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.
@en
prefLabel
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.
@ast
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.
@en
P2860
P356
P1433
P1476
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.
@en
P2093
Anton V Bryksin
Ichiro Matsumura
P2860
P304
P356
10.2144/000113418
P577
2010-06-01T00:00:00Z