Optimization of genome engineering approaches with the CRISPR/Cas9 system. - Wikidata - wikimedia - TriplyDB

Optimization of genome engineering approaches with the CRISPR/Cas9 system.

Optimization of genome engineering approaches with the CRISPR/Cas9 system.

http://www.wikidata.org/entity/Q35233785

about

Genetic spell-checking: gene editing using single-stranded DNA oligonucleotides Efficient Recreation of t(11;22) EWSR1-FLI1+ in Human Stem Cells Using CRISPR/Cas9.CRISPR/Cas9 Immune System as a Tool for Genome Engineering.An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis thaliana.Pervasive Genotypic Mosaicism in Founder Mice Derived from Genome Editing through Pronuclear Injection Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering.Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi.Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System.Conservative site-specific and single-copy transgenesis in human LINE-1 elements.Rosa26-targeted sheep gene knock-in via CRISPR-Cas9 system.Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency.Highly Efficient CRISPR/Cas9-Mediated Homologous Recombination Promotes the Rapid Generation of Bacterial Artificial Chromosomes of Pseudorabies Virus.The RNA-binding landscape of RBM10 and its role in alternative splicing regulation in models of mouse early development.Sequence-specific DNA nicking endonucleases.Mechanisms of gene targeting in higher eukaryotes.Optimizing the DNA Donor Template for Homology-Directed Repair of Double-Strand Breaks.Organoid technologies meet genome engineering.The Convenience of Single Homology Arm Donor DNA and CRISPR/Cas9-Nickase for Targeted Insertion of Long DNA Fragment.Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies.In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9.Precision genome editing in the CRISPR era.ge-CRISPR - An integrated pipeline for the prediction and analysis of sgRNAs genome editing efficiency for CRISPR/Cas system.A Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.CRISPR-Cas9 enables conditional mutagenesis of challenging loci.Pervasive translational regulation of the cell signalling circuitry underlies mammalian development.A platform for reverse genetics in endothelial cells Simultaneous generation of multi-gene knockouts in human cells.Targeted gene editing by transfection of in vitro reconstituted Streptococcus thermophilus Cas9 nuclease complex.Automated microraft platform to identify and collect non-adherent cells successfully gene-edited with CRISPR-Cas9.A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid.Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing.Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection.Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.Efficient gene knockin in axolotl and its use to test the role of satellite cells in limb regeneration.The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones.An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Production of Recombinant Proteins in the Milk of Transgenic Animals: Current State and Prospects

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Optimization of genome engineering approaches with the CRISPR/Cas9 system.

http://www.wikidata.org/entity/Q35233785

description

2014 nî lūn-bûn

@nan

2014 թուականի Օգոստոսին հրատարակուած գիտական յօդուած

@hyw

2014 թվականի օգոստոսին հրատարակված գիտական հոդված

@hy

2014年の論文

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2014年論文

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2014年論文

@zh-hant

2014年論文

@zh-hk

2014年論文

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2014年論文

@zh-tw

2014年论文

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name

Optimization of genome engineering approaches with the CRISPR/Cas9 system.

@ast

Optimization of genome engineering approaches with the CRISPR/Cas9 system.

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type

label

Optimization of genome engineering approaches with the CRISPR/Cas9 system.

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Optimization of genome engineering approaches with the CRISPR/Cas9 system.

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prefLabel

Optimization of genome engineering approaches with the CRISPR/Cas9 system.

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Optimization of genome engineering approaches with the CRISPR/Cas9 system.

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Optimization of genome engineering approaches with the CRISPR/Cas9 system.

@en

P2093

Gang Wang

http://www.w3.org/2001/XMLSchema#string

Kai Li

http://www.w3.org/2001/XMLSchema#string

Pingzhu Zhou

http://www.w3.org/2001/XMLSchema#string

Troels Andersen

http://www.w3.org/2001/XMLSchema#string

William T Pu

http://www.w3.org/2001/XMLSchema#string

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RNA-guided human genome engineering via Cas9

Multiplex genome engineering using CRISPR/Cas systems

Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice

Enzymatic assembly of DNA molecules up to several hundred kilobases

One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering

A highly efficient recombineering-based method for generating conditional knockout mutations

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

DNA targeting specificity of RNA-guided Cas9 nucleases

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

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scholarly article

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10.1371/JOURNAL.PONE.0105779

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