Theoretical design and analysis of multivolume digital assays with wide dynamic range validated experimentally with microfluidic digital PCR - Wikidata - wikimedia - TriplyDB

Theoretical design and analysis of multivolume digital assays with wide dynamic range validated experimentally with microfluidic digital PCR

Theoretical design and analysis of multivolume digital assays with wide dynamic range validated experimentally with microfluidic digital PCR

http://www.wikidata.org/entity/Q35551811

about

Pinch-off of microfluidic droplets with oscillatory velocity of inner phase flow.Designing and interpretation of digital assays: Concentration of target in the sample and in the source of sample Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip.Microfluidics for single-cell genetic analysis Increased robustness of single-molecule counting with microfluidics, digital isothermal amplification, and a mobile phone versus real-time kinetic measurements.Automated generation of libraries of nL droplets.Reading Out Single-Molecule Digital RNA and DNA Isothermal Amplification in Nanoliter Volumes with Unmodified Camera Phones.Highly precise measurement of HIV DNA by droplet digital PCR.Multiplexed quantification of nucleic acids with large dynamic range using multivolume digital RT-PCR on a rotational SlipChip tested with HIV and hepatitis C viral load Control of initiation, rate, and routing of spontaneous capillary-driven flow of liquid droplets through microfluidic channels on SlipChip Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation.Universal microfluidic platform for bioassays in anchored droplets.Optimized droplet digital CFU assay (ddCFU) provides precise quantification of bacteria over a dynamic range of 6 logs and beyond.Lack of correlation between reaction speed and analytical sensitivity in isothermal amplification reveals the value of digital methods for optimization: validation using digital real-time RT-LAMP Comparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirus.Mechanistic evaluation of the pros and cons of digital RT-LAMP for HIV-1 viral load quantification on a microfluidic device and improved efficiency via a two-step digital protocol Quantification of Plasma miRNAs by Digital PCR for Cancer Diagnosis Calibration-free assays on standard real-time PCR devices Quantitative microfluidic biomolecular analysis for systems biology and medicine.Microfluidic multiplexing in bioanalyses.Multiplex Immunoassays Utilizing Differential Affinity Using Aptamers Generated by MARAS.The Future of Digital Polymerase Chain Reaction in Virology.Passive and active droplet generation with microfluidics: a review.Programmable active droplet generation enabled by integrated pneumatic micropumps.A novel picoliter droplet array for parallel real-time polymerase chain reaction based on double-inkjet printing.A poly(dimethylsiloxane) microfluidic sheet reversibly adhered on a glass plate for creation of emulsion droplets for droplet digital PCR.SlipChip Device for Digital Nucleic Acid Amplification.Detection of microRNAs Using Chip-Based QuantStudio 3D Digital PCR.Rapid pathogen-specific phenotypic antibiotic susceptibility testing using digital LAMP quantification in clinical samples.The pumping lid: investigating multi-material 3D printing for equipment-free, programmable generation of positive and negative pressures for microfluidic applications.Should genetic testing go digital?Selecting analytical biomarkers for diagnostic applications: a first principles approach.An integrated slidable and valveless microdevice with solid phase extraction, polymerase chain reaction, and immunochromatographic strip parts for multiplex colorimetric pathogen detection.dPCR: A Technology Review.An epigenetic classifier for early stage lung cancer.

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Theoretical design and analysis of multivolume digital assays with wide dynamic range validated experimentally with microfluidic digital PCR

http://www.wikidata.org/entity/Q35551811

description

2011 nî lūn-bûn

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2011 թուականի Հոկտեմբերին հրատարակուած գիտական յօդուած

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2011 թվականի հոտեմբերին հրատարակված գիտական հոդված

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2011年の論文

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2011年論文

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Theoretical design and analysi ...... with microfluidic digital PCR

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Theoretical design and analysi ...... with microfluidic digital PCR

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Analytical Chemistry

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Theoretical design and analysi ...... with microfluidic digital PCR

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Feng Shen

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Jason E Kreutz

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Rustem F Ismagilov

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Toan Huynh

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Todd Munson

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P2860

Real-time quantification of microRNAs by stem-loop RT-PCR

Digital PCR on a SlipChip

Microfluidic stochastic confinement enhances analysis of rare cells by isolating cells and creating high density environments for control of diffusible signals

Reactions in droplets in microfluidic channels.

Real-time PCR in clinical microbiology: applications for routine laboratory testing.

User-loaded SlipChip for equipment-free multiplexed nanoliter-scale experiments

Advances in developing HIV-1 viral load assays for resource-limited settings

Dead-end filling of SlipChip evaluated theoretically and experimentally as a function of the surface chemistry and the gap size between the plates for lubricated and dry SlipChips.

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