Changes in solvation during DNA binding and cleavage are critical to altered specificity of the EcoRI endonuclease
about
Structure and function of type II restriction endonucleasesTrapping DNA-protein binding reactions with neutral osmolytes for the analysis by gel mobility shift and self-cleavage assays.Quantifying the molecular origins of opposite solvent effects on protein-protein interactionsProbing the role of water in the tryptophan repressor-operator complex.Interfacial water as a "hydration fingerprint" in the noncognate complex of BamHI.Specific versus nonspecific isothermal DNA amplification through thermophilic polymerase and nicking enzyme activities.Dissecting the molecular origins of specific protein-nucleic acid recognition: hydrostatic pressure and molecular dynamics.Differences between EcoRI nonspecific and "star" sequence complexes revealed by osmotic stress.Osmoregulation of dimer resolution at the plasmid pJHCMW1 mwr locus by Escherichia coli XerCD recombination.Sequence-dependent enhancement of hydrolytic deamination of cytosines in DNA by the restriction enzyme PspGI.Effects of 2'-O-methyl nucleotide substitution on EcoRI endonuclease cleavage activitiesStabilizing labile DNA-protein complexes in polyacrylamide gels.DNA tightens the dimeric DNA-binding domain of human papillomavirus E2 protein without changes in volume.Correlated structural kinetics and retarded solvent dynamics at the metalloprotease active siteEstimating hydration changes upon biomolecular reactions from osmotic stress, high pressure, and preferential hydration experiments.Analyzing the forces binding a restriction endonuclease to DNA using a synthetic nanoporeUsing single-turnover kinetics with osmotic stress to characterize the EcoRV cleavage reactionDifferences in hydration coupled to specific and nonspecific competitive binding and to specific DNA Binding of the restriction endonuclease BamHI.The dissociation rate of the EcoRI-DNA-specific complex is linked to water activity.Positive contribution of hydration on DNA binding by E2c protein from papillomavirus.Solvation change and ion release during aminoacylation by aminoacyl-tRNA synthetases.Electrophoretic mobility shift assay (EMSA) for detecting protein-nucleic acid interactions.The role of water molecules in the association of cytochrome P450cam with putidaredoxin. An osmotic pressure study.Temperature and osmotic stress dependence of the thermodynamics for binding linker histone H10, Its carboxyl domain (H10-C) or globular domain (H10-G) to B-DNA.Solvent-induced changes in photochemical activity and conformation of photosystem I particles by glycerol.Ca(2+)-mediated site-specific DNA cleavage and suppression of promiscuous activity of KpnI restriction endonuclease.Enzyme-free and label-free signal amplification for monitoring endonuclease activity and inhibition via hybridization chain reaction.Label-free fluorescent assay for real-time monitoring site-specific DNA cleavage by EcoRI endonuclease.Osmotic pressure effects on EcoRV cleavage and bindingMapping interfacial hydration in ETS-family transcription factor complexes with DNA: a chimeric approachMechanical stability of low-humidity single DNA molecules
P2860
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P2860
Changes in solvation during DNA binding and cleavage are critical to altered specificity of the EcoRI endonuclease
description
1998 nî lūn-bûn
@nan
1998年の論文
@ja
1998年論文
@yue
1998年論文
@zh-hant
1998年論文
@zh-hk
1998年論文
@zh-mo
1998年論文
@zh-tw
1998年论文
@wuu
1998年论文
@zh
1998年论文
@zh-cn
name
Changes in solvation during DN ...... city of the EcoRI endonuclease
@ast
Changes in solvation during DN ...... city of the EcoRI endonuclease
@en
type
label
Changes in solvation during DN ...... city of the EcoRI endonuclease
@ast
Changes in solvation during DN ...... city of the EcoRI endonuclease
@en
prefLabel
Changes in solvation during DN ...... city of the EcoRI endonuclease
@ast
Changes in solvation during DN ...... city of the EcoRI endonuclease
@en
P2860
P356
P1476
Changes in solvation during DN ...... city of the EcoRI endonuclease
@en
P2093
C R Robinson
S G Sligar
P2860
P304
P356
10.1073/PNAS.95.5.2186
P407
P577
1998-03-01T00:00:00Z