High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae.
about
Structure of the human histamine H1 receptor complex with doxepinStructure and mechanism of the mammalian fructose transporter GLUT5Structures of the OmpF porin crystallized in the presence of foscholine-12Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1The yeast plasma membrane ATP binding cassette (ABC) transporter Aus1: purification, characterization, and the effect of lipids on its activity.Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivityDetergent screening and purification of the human liver ABC transporters BSEP (ABCB11) and MDR3 (ABCB4) expressed in the yeast Pichia pastorisAn efficient strategy for small-scale screening and production of archaeal membrane transport proteins in Escherichia coliExpression and Subcellular Distribution of GFP-Tagged Human Tetraspanin Proteins in Saccharomyces cerevisiaeTranslation levels control multi-spanning membrane protein expressionDissecting the proton transport pathway in electrogenic Na(+)/H(+) antiportersSolubilization and characterization of the anthrax toxin pore in detergent micellesInsights into outer membrane protein crystallization.The cystic fibrosis transmembrane conductance regulator (CFTR) and its stabilityOvercoming barriers to membrane protein structure determination.A rapid expression and purification condition screening protocol for membrane protein structural biology.Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications.Reliable high-throughput approach for screening of engineered constitutive promoters in the yeast Pichia pastoris.Tuning microbial hosts for membrane protein production.Direct binding of a plant LysM receptor-like kinase, LysM RLK1/CERK1, to chitin in vitroMethods for Structural and Functional Analyses of Intramembrane Prenyltransferases in the UbiA Superfamily.Computational design of membrane proteins.Detergent screening of the human voltage-gated proton channel using fluorescence-detection size-exclusion chromatography.The recombinant expression systems for structure determination of eukaryotic membrane proteinsFluorescent in situ folding control for rapid optimization of cell-free membrane protein synthesisExpress your LOV: an engineered flavoprotein as a reporter for protein expression and purification.Recombinant production of human Aquaporin-1 to an exceptional high membrane density in Saccharomyces cerevisiae.Ultra-low background DNA cloning system.Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteinsTransmembrane helix straightening and buckling underlies activation of mechanosensitive and thermosensitive K(2P) channels.Overcoming the challenges of membrane protein crystallography.Optimisation of recombinant production of active human cardiac SERCA2a ATPaseAnalysing the substrate multispecificity of a proton-coupled oligopeptide transporter using a dipeptide library.New tools in membrane protein determination.Arginine 199 and leucine 208 have key roles in the control of adenosine A2A receptor signalling function.Overexpression of membrane proteins from higher eukaryotes in yeasts.GFP-based evaluation system of recombinant expression through the secretory pathway in insect cells and its application to the extracellular domains of class C GPCRsMiRNA mimic screen for improved expression of functional neurotensin receptor from HEK293 cellsCassette series designed for live-cell imaging of proteins and high-resolution techniques in yeast.Decreased secretion and unfolded protein response upregulation.
P2860
Q24595968-EB3E938A-E2C5-48D1-92F0-C2FCAB4C6988Q27315967-FE562BE1-6C4E-432E-B206-8DE0F811B0AAQ27660085-749DBEB3-9188-4B4A-9AB2-49FF5AD88BCBQ27681921-31ED44F7-C0BE-4C43-95B9-659CC9B3EE75Q27931974-E621EC04-D93D-419E-8D4C-73918DF36626Q28295016-E056F9A5-595C-49C5-A255-537FE4128314Q28485995-49BEA7F7-81D4-4FE2-BAC1-A80C08DEDECBQ28535378-4F947413-577E-4FB3-8CC8-CF5B24B53FF0Q28546836-4CEFD09E-0B60-4814-90DF-865C75BE68E1Q28730315-2BF9EC58-4501-4880-B4FC-CF33F9AA7C52Q28941782-F7E050BD-A0EB-478D-8CB1-0F716C7E263CQ30157192-8A53F70E-4BDF-4128-85B7-C2BDCA8B8793Q30157457-4C692CB4-8607-48A9-ABF7-A321412108D3Q30394029-5D7D575C-3A67-4706-8883-38B9F8CB1982Q30401692-47F26394-B9B5-4426-8C96-1F9DDB828475Q30402414-4774B4F3-8657-47DB-B047-081BD0A24A52Q30414571-A3A54E2C-F3FC-4AE9-A651-F086D133ADEFQ31004559-D5F00DC5-242D-4CA9-A8CB-43053868438AQ33596003-3DD1CBB7-9BDA-4B61-88CC-E05CDBF98095Q33661555-4ADA6BF0-2DCA-4071-B250-CBD195ED6320Q33688223-8559A92B-C63E-40A9-8118-7A12E062AA82Q33942265-AB60DCAE-8C5F-48C2-93E1-A71E756AEF60Q33977849-31471B04-1D46-4BB0-87B1-0FF8B28B66C7Q34090835-BDFA5397-5856-4184-97D7-8ACCF3213AD4Q34359630-FBD277C5-1163-411A-9028-84A032315377Q34541465-2B26FC58-E3AF-4BB0-9A7D-C2E427D61C23Q34586935-FF3A4B72-FAF6-4F1B-856F-E969D2BF9091Q34586975-F1FFC344-3B22-4586-83F4-E54B0B6EED0CQ34712509-45FD493D-2276-4B4D-87B5-294E4AB1A6AFQ34734923-F3B8E4F7-C933-425C-81E7-04F3EEC1085FQ34803434-57DBD10A-35DA-49C4-BB0E-6DBAA060F58AQ34950754-7DBF72DD-1FA2-4AC0-91B7-2F475B4AA306Q34995844-4C4F7F81-4A8C-4123-8345-A4B5D128A547Q35002005-F314981E-2E19-4D88-9943-2A4AA987F2C2Q35111219-A0FD78F1-CFC0-44B4-BAC7-65552DF12647Q35214601-03203492-FBA4-42C6-9853-CCEDA682CC29Q35556404-57DC353E-1A5A-4CED-8BE4-6D2D8B217DB7Q35560091-A33BC4EE-3460-4476-8791-79859C9F477BQ36020765-6CC5866B-EA93-4D29-9B4F-A38B856623C6Q36031460-8219F991-094F-473F-ADF2-7D384A44CFFA
P2860
High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae.
description
2007 nî lūn-bûn
@nan
2007年の論文
@ja
2007年論文
@yue
2007年論文
@zh-hant
2007年論文
@zh-hk
2007年論文
@zh-mo
2007年論文
@zh-tw
2007年论文
@wuu
2007年论文
@zh
2007年论文
@zh-cn
name
High-throughput fluorescent-ba ...... n in Saccharomyces cerevisiae.
@ast
High-throughput fluorescent-ba ...... n in Saccharomyces cerevisiae.
@en
type
label
High-throughput fluorescent-ba ...... n in Saccharomyces cerevisiae.
@ast
High-throughput fluorescent-ba ...... n in Saccharomyces cerevisiae.
@en
prefLabel
High-throughput fluorescent-ba ...... n in Saccharomyces cerevisiae.
@ast
High-throughput fluorescent-ba ...... n in Saccharomyces cerevisiae.
@en
P2860
P50
P356
P1476
High-throughput fluorescent-ba ...... n in Saccharomyces cerevisiae.
@en
P2093
P2860
P304
13936-13941
P356
10.1073/PNAS.0704546104
P407
P577
2007-08-20T00:00:00Z