Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in Tetrahymena thermophila
about
MEC-17 is an alpha-tubulin acetyltransferaseElucidation of clathrin-mediated endocytosis in tetrahymena reveals an evolutionarily convergent recruitment of dynaminKinesin-II is preferentially targeted to assembling cilia and is required for ciliogenesis and normal cytokinesis in TetrahymenaFAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein cCCTalpha and CCTdelta chaperonin subunits are essential and required for cilia assembly and maintenance in Tetrahymena.Elimination of foreign DNA during somatic differentiation in Tetrahymena thermophila shows position effect and is dosage dependent.RAD51 is required for propagation of the germinal nucleus in Tetrahymena thermophila.A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila.A knockout mutation of a constitutive GPCR in Tetrahymena decreases both G-protein activity and chemoattraction.The CNA1 histone of the ciliate Tetrahymena thermophila is essential for chromosome segregation in the germline micronucleusα-Tubulin mutations alter oryzalin affinity and microtubule assembly properties to confer dinitroaniline resistance.Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis.The nonessential H2A N-terminal tail can function as an essential charge patch on the H2A.Z variant N-terminal tailExcision of micronuclear-specific DNA requires parental expression of pdd2p and occurs independently from DNA replication in Tetrahymena thermophilaChromodomain protein Tcd1 is required for macronuclear genome rearrangement and repair in TetrahymenaHistone H3 lysine 9 methylation is required for DNA elimination in developing macronuclei in Tetrahymena.Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers.Polyglycylation of tubulin is essential and affects cell motility and division in Tetrahymena thermophila.The two human centrin homologues have similar but distinct functions at Tetrahymena basal bodies.Different effects of Tetrahymena IFT172 domains on anterograde and retrograde intraflagellar transport.Analysis of exocytosis mutants indicates close coupling between regulated secretion and transcription activation in Tetrahymena.Glutamylation on alpha-tubulin is not essential but affects the assembly and functions of a subset of microtubules in Tetrahymena thermophilaTubulin polyglycylation: differential posttranslational modification of dynamic cytoplasmic and stable axonemal microtubules in paramecium.Tetrahymena as a Unicellular Model Eukaryote: Genetic and Genomic Tools.Dynein-2 affects the regulation of ciliary length but is not required for ciliogenesis in Tetrahymena thermophilaImpaired replication elongation in Tetrahymena mutants deficient in histone H3 Lys 27 monomethylation.Histone H2B ubiquitylation is not required for histone H3 methylation at lysine 4 in tetrahymena.Quantitative proteomics reveals histone modifications in crosstalk with H3 lysine 27 methylation.Deposition and function of histone H3 variants in Tetrahymena thermophila.Hypoxia regulates assembly of cilia in suppressors of Tetrahymena lacking an intraflagellar transport subunit gene.Centromeric histone H3 is essential for vegetative cell division and for DNA elimination during conjugation in Tetrahymena thermophila.Mapping the germ-line and somatic genomes of a ciliated protozoan, Tetrahymena thermophila.PHLP2 is essential and plays a role in ciliogenesis and microtubule assembly in Tetrahymena thermophila.Molecular genetic analysis of an SNF2/brahma-related gene in Tetrahymena thermophila suggests roles in growth and nuclear development.Chlamydomonas: the cell and its genomes.A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement.
P2860
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P2860
Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in Tetrahymena thermophila
description
1997 nî lūn-bûn
@nan
1997年の論文
@ja
1997年論文
@yue
1997年論文
@zh-hant
1997年論文
@zh-hk
1997年論文
@zh-mo
1997年論文
@zh-tw
1997年论文
@wuu
1997年论文
@zh
1997年论文
@zh-cn
name
Germ-line knockout heterokaryo ...... lei in Tetrahymena thermophila
@ast
Germ-line knockout heterokaryo ...... lei in Tetrahymena thermophila
@en
type
label
Germ-line knockout heterokaryo ...... lei in Tetrahymena thermophila
@ast
Germ-line knockout heterokaryo ...... lei in Tetrahymena thermophila
@en
prefLabel
Germ-line knockout heterokaryo ...... lei in Tetrahymena thermophila
@ast
Germ-line knockout heterokaryo ...... lei in Tetrahymena thermophila
@en
P2860
P356
P1476
Germ-line knockout heterokaryo ...... lei in Tetrahymena thermophila
@en
P2093
P2860
P304
P356
10.1073/PNAS.94.4.1310
P407
P577
1997-02-01T00:00:00Z