Overexpression of bacterio-opsin in Escherichia coli as a water-soluble fusion to maltose binding protein: efficient regeneration of the fusion protein and selective cleavage with trypsin

Overexpression of bacterio-opsin in Escherichia coli as a water-soluble fusion to maltose binding protein: efficient regeneration of the fusion protein and selective cleavage with trypsin

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http://www.wikidata.org/entity/Q36279691
Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fusedHigh overexpression and purification of optimized bacterio-opsin from Halobacterium Salinarum R1 in E. coliA synergistic approach to protein crystallization: combination of a fixed-arm carrier with surface entropy reduction.A systematic assessment of mature MBP in membrane protein production: overexpression, membrane targeting and purification.Applications of novel affinity cassette methods: use of peptide fusion handles for the purification of recombinant proteins.Overexpression of a glutamate receptor (GluR2) ligand binding domain in Escherichia coli: application of a novel protein folding screen.Crystal structures of fusion proteins with large-affinity tags.An Approach to Heterologous Expression of Membrane Proteins. The Case of Bacteriorhodopsin.Soluble oligomers of the intramembrane serine protease YqgP are catalytically active in the absence of detergents.Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein.Bacteriorhodopsin chimeras containing the third cytoplasmic loop of bovine rhodopsin activate transducin for GTP/GDP exchange.Probing the ligand binding domain of the GluR2 receptor by proteolysis and deletion mutagenesis defines domain boundaries and yields a crystallizable constructEvidence for post-translational membrane insertion of the integral membrane protein bacterioopsin expressed in the heterologous halophilic archaeon Haloferax volcanii.
P2860

Overexpression of bacterio-opsin in Escherichia coli as a water-soluble fusion to maltose binding protein: efficient regeneration of the fusion protein and selective cleavage with trypsin

Item
http://www.wikidata.org/entity/Q36279691
description
1996 nî lūn-bûn
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1996年の論文
@ja
1996年学术文章
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1996年学术文章
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1996年学术文章
@zh-hans
1996年学术文章
@zh-my
1996年学术文章
@zh-sg
1996年學術文章
@yue
1996年學術文章
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1996年學術文章
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name
Overexpression of bacterio-ops ...... elective cleavage with trypsin
@ast
Overexpression of bacterio-ops ...... elective cleavage with trypsin
@en
type
label
Overexpression of bacterio-ops ...... elective cleavage with trypsin
@ast
Overexpression of bacterio-ops ...... elective cleavage with trypsin
@en
prefLabel
Overexpression of bacterio-ops ...... elective cleavage with trypsin
@ast
Overexpression of bacterio-ops ...... elective cleavage with trypsin
@en
P1433
P1476
P2093
P2860
P304
P31
P356
P433
P478
P577
P5875
P698
P921
P932
P356
P1433
P1476
Overexpression of bacterio-ops ...... elective cleavage with trypsin
@en
P2093
P2860
P304
P31
P356
P433
P478
P577
P5875
P698
P921
P932