Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.
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Plasmid RSF1010 DNA replication in vitro promoted by purified RSF1010 RepA, RepB and RepC proteinsDNA synthesis is initiated at two positions within the origin of replication of plasmid R1162.Dynamics of the ssDNA recognition by the RepA hexameric helicase of plasmid RSF1010: analyses using fluorescence stopped-flow intensity and anisotropy methods.Mechanisms of interactions of the nucleotide cofactor with the RepA protein of plasmid RSF1010. Binding dynamics studied using the fluorescence stopped-flow methodReplication of the broad host range plasmid RSF1010: requirement for three plasmid-encoded proteinsTwo domains at the origin are required for replication and maintenance of broad-host-range plasmid R1162.Properties of R1162, a broad-host-range, high-copy-number plasmidTn3 labeling of a cryptic plasmid found in the plant pathogenic bacterium Pseudomonas tabaci and mobilization of RSF1010 by donation.Location of an ampicillin resistance transposon, Tn1701, in a group of small, nontransferring plasmids.Origin and direction of DNA replication of plasmid RSF1030.Functional division and reconstruction of a plasmid replication origin: molecular dissection of the oriV of the broad-host-range plasmid RSF1010Replication and conjugative mobilization of broad host-range IncQ plasmids.Protein RepC is involved in copy number control of the broad host range plasmid RSF1010.Replication of plasmids in gram-negative bacteria.Replication and control of circular bacterial plasmids.Molecular nature of a streptomycin and sulfonamide resistance plasmid (pBP1) prevalent in clinical Escherichia coli strains and integration of an ampicillin resistance transposon (TnA).Three clustered origins of replication in a promiscuous-plasmid replicon and their differential use in a PolA+ strain and a delta PolA strain of Escherichia coli K-12.Construction of tn3-containing plasmids from plant-pathogenic pseudomonads and an examination of their biological properties.Location of two relaxation nick sites in R6K and single sites in pSC101 and RSF1010 close to origins of vegetative replication: implication for conjugal transfer of plasmid deoxyribonucleic acidFunctional difference between the two oppositely oriented priming signals essential for the initiation of the broad host-range plasmid RSF1010 DNA replication.DnaG-dependent priming signals can substitute for the two essential DNA initiation signals in oriV of the broad host-range plasmid RSF1010.Replication of the broad host range plasmid RSF1010 in cell-free extracts of Escherichia coli and Pseudomonas aeruginosa.Gene F of plasmid RSF1010 codes for a low-molecular-weight repressor protein that autoregulates expression of the repAC operon.Multiple global conformational states of the hexameric RepA helicase of plasmid RSF1010 with different ssDNA-binding capabilities are induced by different numbers of bound nucleotides. Analytical ultracentrifugation and dynamic light scattering stud
P2860
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P2860
Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.
description
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name
Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.
@ast
Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.
@en
type
label
Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.
@ast
Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.
@en
prefLabel
Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.
@ast
Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.
@en
P2093
P2860
P1476
Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.
@en
P2093
P2860
P304
P407
P577
1978-06-01T00:00:00Z