about
Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complexProduction of glycoprotein vaccines in Escherichia coliHigh overexpression and purification of optimized bacterio-opsin from Halobacterium Salinarum R1 in E. coliGenetically distinct pathways guide effector export through the type VI secretion systemThe periplasmic enzyme, AnsB, of Shigella flexneri modulates bacterial adherence to host epithelial cellsThe pancreatitis-induced vacuole membrane protein 1 triggers autophagy in mammalian cellsTranslation levels control multi-spanning membrane protein expressionA systematic assessment of mature MBP in membrane protein production: overexpression, membrane targeting and purification.A TSPO-related protein localizes to the early secretory pathway in Arabidopsis, but is targeted to mitochondria when expressed in yeast.The neurobiologist's guide to structural biology: a primer on why macromolecular structure matters and how to evaluate structural data.Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis.Membrane damage elicits an immunomodulatory program in Staphylococcus aureusSimple genetic selection protocol for isolation of overexpressed genes that enhance accumulation of membrane-integrated human G protein-coupled receptors in Escherichia coliProduction and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system.Comparative lipidomics of azole sensitive and resistant clinical isolates of Candida albicans reveals unexpected diversity in molecular lipid imprints.Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris.Heterologous expression of membrane proteins: choosing the appropriate host.Multi-copy genes that enhance the yield of mammalian G protein-coupled receptors in Escherichia coli.High throughput identification of monoclonal antibodies to membrane bound and secreted proteins using yeast and phage display.Identification and characterization of a novel porin family highlights a major difference in the outer membrane of chlamydial symbionts and pathogens.Using Haloarcula marismortui bacteriorhodopsin as a fusion tag for enhancing and visible expression of integral membrane proteins in Escherichia coli.Live imaging of endogenous PSD-95 using ENABLED: a conditional strategy to fluorescently label endogenous proteins.Detailed topology mapping reveals substantial exposure of the "cytoplasmic" C-terminal tail (CTT) sequences in HIV-1 Env proteins at the cell surface.Overcoming the challenges of membrane protein crystallography.Tat peptide-mediated soluble expression of the membrane protein LSECtin-CRD in Escherichia coliGeneration of recombinant antibodies to rat GABAA receptor subunits by affinity selection on synthetic peptidesOverexpression of membrane proteins from higher eukaryotes in yeasts.Making water-soluble integral membrane proteins in vivo using an amphipathic protein fusion strategy.MiRNA mimic screen for improved expression of functional neurotensin receptor from HEK293 cellsDetermining the N-terminal orientations of recombinant transmembrane proteins in the Escherichia coli plasma membrane.Fluorogen activating proteins in flow cytometry for the study of surface molecules and receptorsStructure-guided SCHEMA recombination generates diverse chimeric channelrhodopsinsTowards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes.Purification of recombinant G-protein-coupled receptors.Efficient production of membrane-integrated and detergent-soluble G protein-coupled receptors in Escherichia coli.Membrane protein structural biology--how far can the bugs take us?Tuning Escherichia coli for membrane protein overexpressionEfficient cell-free production of olfactory receptors: detergent optimization, structure, and ligand binding analyses.A Link between Integral Membrane Protein Expression and Simulated Integration EfficiencyRevolutionizing membrane protein overexpression in bacteria.
P2860
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P2860
description
2006 nî lūn-bûn
@nan
2006年の論文
@ja
2006年論文
@yue
2006年論文
@zh-hant
2006年論文
@zh-hk
2006年論文
@zh-mo
2006年論文
@zh-tw
2006年论文
@wuu
2006年论文
@zh
2006年论文
@zh-cn
name
Rationalizing membrane protein overexpression.
@ast
Rationalizing membrane protein overexpression.
@en
type
label
Rationalizing membrane protein overexpression.
@ast
Rationalizing membrane protein overexpression.
@en
prefLabel
Rationalizing membrane protein overexpression.
@ast
Rationalizing membrane protein overexpression.
@en
P2093
P1476
Rationalizing membrane protein overexpression.
@en
P2093
David Drew
Jan-Willem de Gier
Mirjam Lerch Bader
Samuel Wagner
P304
P356
10.1016/J.TIBTECH.2006.06.008
P577
2006-07-03T00:00:00Z