Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolution.
about
Integrated recombinant protein expression and purification platform based on Ralstonia eutropha.The intein of the Thermoplasma A-ATPase A subunit: structure, evolution and expression in E. coliNature's recipe for splitting inteinsProbing the structure of the PI-SceI-DNA complex by affinity cleavage and affinity photocross-linkingCrystal structure of a mini-intein reveals a conserved catalytic module involved in side chain cyclization of asparagine during protein splicingCrystallographic and Mutational Studies of Mycobacterium tuberculosis recA Mini-inteins Suggest a Pivotal Role for a Highly Conserved Aspartate ResidueSelection and Structure of Hyperactive Inteins: Peripheral Changes Relayed to the Catalytic Centerp K a Coupling at the Intein Active Site: Implications for the Coordination Mechanism of Protein Splicing with a Conserved AspartateIn vivo and in vitro protein ligation by naturally occurring and engineered split DnaE inteinsMycobacterium tuberculosis RecA intein possesses a novel ATP-dependent site-specific double-stranded DNA endonuclease activityUnusual evolutionary history of the tRNA splicing endonuclease EndA: relationship to the LAGLIDADG and PD-(D/E)XK deoxyribonucleasesProtein trans-splicing as a protein ligation tool to study protein structure and function.Metal ions binding to recA inteins from Mycobacterium tuberculosis.Spontaneous proton transfer to a conserved intein residue determines on-pathway protein splicing.The Mycobacterium tuberculosis recA intein can be used in an ORFTRAP to select for open reading frames.Adaptation for horizontal transfer in a homing endonuclease.Protein splicing in vitro with a semisynthetic two-component minimal intein.Enigmatic distribution, evolution, and function of inteinsProtein splicing: how inteins escape from precursor proteins.Bacterial biosensors for evaluating potential impacts of estrogenic endocrine disrupting compounds in multiple species.An intein with genetically selectable markers provides a new approach to internally label proteins with GFPBackbone dynamics and global effects of an activating mutation in minimized Mtu RecA inteins.Protein and RNA engineering to customize microbial molecular reporting.Rapid detection of subtype-selective nuclear hormone receptor binding with bacterial genetic selectionIntein-mediated purification of cytotoxic endonuclease I-TevI by insertional inactivation and pH-controllable splicingCisplatin inhibits protein splicing, suggesting inteins as therapeutic targets in mycobacteria.Temperature-sensitive mutations made easy: generating conditional mutations by using temperature-sensitive inteins that function within different temperature rangesDynamics differentiate between active and inactive inteins.Cyclization of the intrinsically disordered α1S dihydropyridine receptor II-III loop enhances secondary structure and in vitro function.Lightning strikes twice: intron-intein coincidenceProtein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803Group I introns and inteins: disparate origins but convergent parasitic strategies.Inteins, valuable genetic elements in molecular biology and biotechnology.The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding.Regulation of protein activity with small-molecule-controlled inteins.Split-inteins and their bioapplications.Engineering and optimization of an allosteric biosensor protein for peroxisome proliferator-activated receptor γ ligands.Intronic GIY-YIG endonuclease gene in the mitochondrial genome of Podospora curvicolla: evidence for mobility.Homology modeling and mutational analysis of Ho endonuclease of yeast.1H, 13C, and 15N NMR assignments of an engineered intein based on Mycobacterium tuberculosis RecA.
P2860
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P2860
Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolution.
description
1997 nî lūn-bûn
@nan
1997年の論文
@ja
1997年論文
@yue
1997年論文
@zh-hant
1997年論文
@zh-hk
1997年論文
@zh-mo
1997年論文
@zh-tw
1997年论文
@wuu
1997年论文
@zh
1997年论文
@zh-cn
name
Genetic definition of a protei ...... a model for intein evolution.
@ast
Genetic definition of a protei ...... a model for intein evolution.
@en
type
label
Genetic definition of a protei ...... a model for intein evolution.
@ast
Genetic definition of a protei ...... a model for intein evolution.
@en
prefLabel
Genetic definition of a protei ...... a model for intein evolution.
@ast
Genetic definition of a protei ...... a model for intein evolution.
@en
P2093
P2860
P356
P1476
Genetic definition of a protei ...... a model for intein evolution.
@en
P2093
P2860
P304
11466-11471
P356
10.1073/PNAS.94.21.11466
P407
P577
1997-10-01T00:00:00Z