GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae.
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Large-scale production and protein engineering of G protein-coupled receptors for structural studiesStructure and mechanism of the mammalian fructose transporter GLUT5Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBTA two-domain elevator mechanism for sodium/proton antiportMolecular basis of nitrate uptake by the plant nitrate transporter NRT1.1The yeast plasma membrane ATP binding cassette (ABC) transporter Aus1: purification, characterization, and the effect of lipids on its activity.Saccharomyces cerevisiae-based platform for rapid production and evaluation of eukaryotic nutrient transporters and transceptors for biochemical studies and crystallographyHigh-throughput recombinant protein expression in Escherichia coli: current status and future perspectivesDrosophila photoreceptor cells exploited for the production of eukaryotic membrane proteins: receptors, transporters and channelsDetergent screening and purification of the human liver ABC transporters BSEP (ABCB11) and MDR3 (ABCB4) expressed in the yeast Pichia pastorisThe pCri System: a vector collection for recombinant protein expression and purificationExpression and Subcellular Distribution of GFP-Tagged Human Tetraspanin Proteins in Saccharomyces cerevisiaeOil accumulation by the oleaginous diatom Fistulifera solaris as revealed by the genome and transcriptomeEngineering vanilloid-sensitivity into the rat TRPV2 channelIntegrating mass spectrometry with MD simulations reveals the role of lipids in Na(+)/H(+) antiportersDissecting the proton transport pathway in electrogenic Na(+)/H(+) antiportersInsights into outer membrane protein crystallization.A rapid expression and purification condition screening protocol for membrane protein structural biology.Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications.Tuning microbial hosts for membrane protein production.Accessible Mannitol-Based Amphiphiles (MNAs) for Membrane Protein Solubilisation and StabilisationComputational design of membrane proteins.Detergent screening of the human voltage-gated proton channel using fluorescence-detection size-exclusion chromatography.Expression of a biologically active GFP-α(S1)-casein fusion protein in Lactococcus lactis.Detergent-induced stabilization and improved 3D map of the human heteromeric amino acid transporter 4F2hc-LAT2.Golgi anti-apoptotic proteins are highly conserved ion channels that affect apoptosis and cell migration.Recombinant production of human Aquaporin-1 to an exceptional high membrane density in Saccharomyces cerevisiae.Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteinsTransmembrane helix straightening and buckling underlies activation of mechanosensitive and thermosensitive K(2P) channels.Fluorescent probe for high-throughput screening of membrane protein expressionExperimental detection of proteolytic activity in a signal peptide peptidase of Arabidopsis thaliana.Overcoming the challenges of membrane protein crystallography.Optimisation of recombinant production of active human cardiac SERCA2a ATPaseAnalysing the substrate multispecificity of a proton-coupled oligopeptide transporter using a dipeptide library.Nanobody mediated crystallization of an archeal mechanosensitive channel.High yield purification of full-length functional hERG K+ channels produced in Saccharomyces cerevisiae.Dodecyl maltopyranoside enabled purification of active human GABA type A receptors for deep and direct proteomic sequencingThe Aspergillus nidulans proline permease as a model for understanding the factors determining substrate binding and specificity of fungal amino acid transporters.Overexpression of membrane proteins from higher eukaryotes in yeasts.Stubborn contaminants: influence of detergents on the purity of the multidrug ABC transporter BmrA.
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P2860
GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae.
description
article científic
@ca
article scientifique
@fr
articolo scientifico
@it
artigo científico
@pt
bilimsel makale
@tr
scientific article published on January 2008
@en
vedecký článok
@sk
vetenskaplig artikel
@sv
videnskabelig artikel
@da
vědecký článek
@cs
name
GFP-based optimization scheme ...... s in Saccharomyces cerevisiae.
@en
GFP-based optimization scheme ...... s in Saccharomyces cerevisiae.
@nl
type
label
GFP-based optimization scheme ...... s in Saccharomyces cerevisiae.
@en
GFP-based optimization scheme ...... s in Saccharomyces cerevisiae.
@nl
prefLabel
GFP-based optimization scheme ...... s in Saccharomyces cerevisiae.
@en
GFP-based optimization scheme ...... s in Saccharomyces cerevisiae.
@nl
P2093
P2860
P50
P356
P1433
P1476
GFP-based optimization scheme ...... s in Saccharomyces cerevisiae.
@en
P2093
P2860
P2888
P304
P356
10.1038/NPROT.2008.44
P577
2008-01-01T00:00:00Z
P5875
P6179
1011300232