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Can tissue-based immune markers be used for studying the natural history of cancer?A ChIP on the shoulder? Chromatin immunoprecipitation and validation strategies for ChIP antibodiesFacilitating the Validation of Novel Protein Biomarkers for Dementia: An Optimal Workflow for the Development of Sandwich ImmunoassaysMethodological requirements for valid tissue-based biomarker studies that can be used in clinical practiceGarbage in, garbage out: a critical evaluation of strategies used for validation of immunohistochemical biomarkersReconciling the Structural Attributes of Avian AntibodiesA roadmap to generate renewable protein binders to the human proteomeMetastasis suppressor genes at the interface between the environment and tumor cell growthThe use of sensitive chemical antibodies for diagnosis: detection of low levels of EpCAM in breast cancerClaudin-2 Expression Levels in Ulcerative Colitis: Development and Validation of an In-Situ Hybridisation Assay for Therapeutic StudiesSurvey statistics of automated segmentations applied to optical imaging of mammalian cells.A seven-marker signature and clinical outcome in malignant melanoma: a large-scale tissue-microarray study with two independent patient cohortsImpact of immunization technology and assay application on antibody performance--a systematic comparative evaluationVersatile, sensitive liquid chromatography mass spectrometry - Implementation of 10 μm OT columns suitable for small molecules, peptides and proteinsDetermination of equilibrium dissociation constants for recombinant antibodies by high-throughput affinity electrophoresis.Purification of mitochondrial proteins HSP60 and ATP synthase from ascidian eggs: implications for antibody specificity.Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs) to Monitor Compound Effects on Cardiac Myocyte Signaling Pathways.Improved data normalization methods for reverse phase protein microarray analysis of complex biological samples.Issues associated with the use of phosphospecific antibodies to localise active and inactive pools of GSK-3 in cellsHitchhiker antigens: inconsistent ChIP results, questionable immunohistology data, and poor antibody performance may have a common factor.Pan-cancer analysis for studying cancer stage using protein expression dataDesign and operation of an automated high-throughput monoclonal antibody facility.The availability of a recombinant anti-SNAP antibody in VHH format amplifies the application flexibility of SNAP-tagged proteins.A conditional knockout mouse model reveals endothelial cells as the principal and possibly exclusive source of plasma factor VIII.In situ quantitative measurement of HER2mRNA predicts benefit from trastuzumab-containing chemotherapy in a cohort of metastatic breast cancer patientsBeyond natural antibodies: the power of in vitro display technologies.Affimer proteins are versatile and renewable affinity reagentsChoose and Use Your Chemical Probe Wisely to Explore Cancer Biology.Cellular distribution and subcellular localization of mCLCA1/2 in murine gastrointestinal epithelia.Multiplexed ion beam imaging of human breast tumorsThe effect of erythropoietin on normal and neoplastic cells.Benefits and pitfalls of secondary antibodies: why choosing the right secondary is of primary importanceQuantitative immunofluorescence microscopy of subcellular GLUT4 distribution in human skeletal muscle: effects of endurance and sprint interval training.Expanded clinical and experimental use of SOX11 - using a monoclonal antibodyWhen tissue antigens and antibodies get along: revisiting the technical aspects of immunohistochemistry--the red, brown, and blue technique.Application of immunohistochemistry in stereology for quantitative assessment of neural cell populations illustrated in the Göttingen minipig.RNAi-based validation of antibodies for reverse phase protein arrays.Association of hyaluronic acid family members (HAS1, HAS2, and HYAL-1) with bladder cancer diagnosis and prognosisEpo receptors are not detectable in primary human tumor tissue samplesAn improved single-chain Fab platform for efficient display and recombinant expression.
P2860
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P2860
description
article científic
@ca
article scientifique
@fr
articolo scientifico
@it
artigo científico
@pt
bilimsel makale
@tr
scientific article published on March 2010
@en
vedecký článok
@sk
vetenskaplig artikel
@sv
videnskabelig artikel
@da
vědecký článek
@cs
name
Antibody validation
@en
Antibody validation.
@nl
type
label
Antibody validation
@en
Antibody validation.
@nl
prefLabel
Antibody validation
@en
Antibody validation.
@nl
P2093
P2860
P356
P1433
P1476
Antibody validation
@en
P2093
Allison Welsh
David Rimm
Elizabeth Killiam
Jennifer Bordeaux
Maria Baquero
Seema Agarwal
Valsamo Anagnostou
P2860
P304
P356
10.2144/000113382
P50
P577
2010-03-01T00:00:00Z