Mutations in direct repeat sequences and in a conserved sequence adjacent to the repeats result in a defective replication origin in plasmid R6K.
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Copy-number of broad host-range plasmid R1162 is regulated by a small RNAReplisome Assembly at Bacterial Chromosomes and Iteron PlasmidsThe final stages of spliceosome maturation require Spp2p that can interact with the DEAH box protein Prp2p and promote step 1 of splicing.Two forms of replication initiator protein: positive and negative controls.Dimers of pi protein bind the A+T-rich region of the R6K gamma origin near the leading-strand synthesis start sites: regulatory implicationsAT-rich region and repeated sequences - the essential elements of replication origins of bacterial replicons.Negative control of plasmid R6K replication: possible role of intermolecular coupling of replication origins.Initiator protein pi can bind independently to two domains of the gamma origin core of plasmid R6K: the direct repeats and the A+T-rich segmentCharacterization of the basic replicon of pCM1, a narrow-host-range plasmid from the moderate halophile Chromohalobacter marismortui.A DNA segment conferring stable maintenance on R6K gamma-origin core replicons.Preponderance of Fis-binding sites in the R6K gamma origin and the curious effect of the penicillin resistance marker on replication of this origin in the absence of FisStructure-based functional analysis of the replication protein of plasmid R6K: key amino acids at the pi/DNA interfaceBinding of DnaA protein to a replication enhancer counteracts the inhibition of plasmid R6K gamma origin replication mediated by elevated levels of R6K pi proteinThe integration host factor of Escherichia coli binds to multiple sites at plasmid R6K gamma origin and is essential for replication.Replication origin mutations affecting binding of pSC101 plasmid-encoded Rep initiator proteinIdentification of a novel promoter in the replication control region of plasmid R6KCooperative binding mode of the inhibitors of R6K replication, pi dimers.Plasmid R6K replication controlStructural and functional analysis of a replication enhancer: separation of the enhancer activity from origin function by mutational dissection of the replication origin gamma of plasmid R6KSequence-specific interaction between the replication initiator protein of plasmid pT181 and its origin of replication.Positive and negative roles of an initiator protein at an origin of replication.Reconstitution of R6K DNA replication in vitro using 22 purified proteins.Some factors affecting the copy number of specific plasmids in Bacillus species.DNA-binding domain of the RepE initiator protein of mini-F plasmid: involvement of the carboxyl-terminal regionAltered (copy-up) forms of initiator protein pi suppress the point mutations inactivating the gamma origin of plasmid R6K.Minimal essential origin of plasmid pSC101 replication: requirement of a region downstream of iterons.Integration host factor of Escherichia coli reverses the inhibition of R6K plasmid replication by pi initiator protein.The interaction of RepC initiator with iterons in the replication of the broad host-range plasmid RSF1010Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K.Cooperative binding of initiator protein to replication origin conferred by single amino acid substitution.Mechanism of origin activation by monomers of R6K-encoded pi protein.A single amino acid alteration in the initiation protein is responsible for the DNA overproduction phenotype of copy number mutants of plasmid R6K.The replication origin of the Methylomonas clara plasmid pBE-2.A novel Bacillus natto plasmid pLS32 capable of replication in Bacillus subtilis.Binding modes of the initiator and inhibitor forms of the replication protein pi to the gamma ori iteron of plasmid R6K.
P2860
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P2860
Mutations in direct repeat sequences and in a conserved sequence adjacent to the repeats result in a defective replication origin in plasmid R6K.
description
article científic
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article scientifique
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articolo scientifico
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artigo científico
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bilimsel makale
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scientific article published on March 1985
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vedecký článok
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vetenskaplig artikel
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videnskabelig artikel
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vědecký článek
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name
Mutations in direct repeat seq ...... ication origin in plasmid R6K.
@en
Mutations in direct repeat seq ...... ication origin in plasmid R6K.
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type
label
Mutations in direct repeat seq ...... ication origin in plasmid R6K.
@en
Mutations in direct repeat seq ...... ication origin in plasmid R6K.
@nl
prefLabel
Mutations in direct repeat seq ...... ication origin in plasmid R6K.
@en
Mutations in direct repeat seq ...... ication origin in plasmid R6K.
@nl
P2093
P2860
P356
P1476
Mutations in direct repeat seq ...... ication origin in plasmid R6K.
@en
P2093
D R Helinski
M Filutowicz
M J McEachern
P2860
P304
P356
10.1073/PNAS.82.5.1480
P407
P577
1985-03-01T00:00:00Z