MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.
about
Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins.Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice.Applications of CRISPR technologies in research and beyond.Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJCo-incident insertion enables high efficiency genome engineering in mouse embryonic stem cells.TALEN-mediated knock-in via non-homologous end joining in the crustacean Daphnia magna.CRISPR-Cas9: a promising tool for gene editing on induced pluripotent stem cells.Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavageProgress and Application of CRISPR/Cas Technology in Biological and Biomedical Investigation.New Mammalian Expression Systems.Homology-mediated end joining-based targeted integration using CRISPR/Cas9.Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO.Genome Editing of Silkworms.A Scalable Epitope Tagging Approach for High Throughput ChIP-Seq Analysis.Enhanced CRISPR/Cas9-mediated biallelic genome targeting with dual surrogate reporter-integrated donors.Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis.Use of genome-editing tools to treat sickle cell disease.Impact of gene editing on the study of cystic fibrosis.Gene Editing: Powerful New Tools for Nephrology Research and Therapy.Regulation of Single-Strand Annealing and its Role in Genome Maintenance.In vivo and in vitro disease modeling with CRISPR/Cas9.Cornerstones of CRISPR-Cas in drug discovery and therapy.Targeted Genome Replacement via Homology-directed Repair in Non-dividing Cardiomyocytes.Generation of Genomic Deletions (of Rig-I GENE) in Goat Primary Cell Culture Using CRISPR/CAS9 Method.An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes).Evaluation of ATM heterozygous mutations underlying individual differences in radiosensitivity using genome editing in human cultured cells.Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated, homology-independent knock-in system.Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms.Simultaneous generation of multi-gene knockouts in human cells.Hematopoietic Stem Cell Gene Therapy: Progress and Lessons Learned.In vivo genome editing via the HITI method as a tool for gene therapy.A Simple and Universal System for Gene Manipulation in Aspergillus fumigatus: In Vitro-Assembled Cas9-Guide RNA Ribonucleoproteins Coupled with Microhomology Repair Templates.Production of knock-in mice in a single generation from embryonic stem cells.Applications of Alternative Nucleases in the Age of CRISPR/Cas9.The role of host DNA ligases in hepadnavirus covalently closed circular DNA formation.Magic wands of CRISPR-lots of choices for gene knock-in.Genetic Tools for Self-Organizing Culture of Mouse Embryonic Stem Cells via Small Regulatory RNA-Mediated Technologies, CRISPR/Cas9, and Inducible RNAi.Control of gene editing by manipulation of DNA repair mechanisms.Updated summary of genome editing technology in human cultured cells linked to human genetics studies.All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells.
P2860
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P2860
MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.
description
2015 nî lūn-bûn
@nan
2015年の論文
@ja
2015年論文
@yue
2015年論文
@zh-hant
2015年論文
@zh-hk
2015年論文
@zh-mo
2015年論文
@zh-tw
2015年论文
@wuu
2015年论文
@zh
2015年论文
@zh-cn
name
MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.
@en
type
label
MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.
@en
prefLabel
MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.
@en
P2860
P50
P356
P1433
P1476
MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems
@en
P2093
Shota Nakade
Yuto Sakane
P2860
P2888
P304
P356
10.1038/NPROT.2015.140
P577
2015-12-17T00:00:00Z
P6179
1047086433