A dual reporter screening system identifies the amino acid at position 82 in Flp site-specific recombinase as a determinant for target specificity.
about
The Flp double cross system a simple efficient procedure for cloning DNA fragmentsExpanding the scope of site-specific recombinases for genetic and metabolic engineeringUnveiling hidden catalytic contributions of the conserved His/Trp-III in tyrosine recombinases: assembly of a novel active site in Flp recombinase harboring alanine at this positionGenetic tools for allelic replacement in Burkholderia species.Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable marker.Directed evolution of recombinase specificity by split gene reassemblyStructure-guided reprogramming of serine recombinase DNA sequence specificityReal-time single-molecule tethered particle motion analysis reveals mechanistic similarities and contrasts of Flp site-specific recombinase with Cre and λ IntEnhancing the specificity of recombinase-mediated genome engineering through dimer interface redesign.Directed evolution of λ integrase activity and specificity by genetic derepression.Evolution of variants of yeast site-specific recombinase Flp that utilize native genomic sequences as recombination target sites.Efficient Genome Manipulation by Variants of Site-Specific Recombinases R and TD.Binding and catalytic contributions to site recognition by flp recombinase.A genome-wide analysis of FRT-like sequences in the human genome.Target-specific variants of Flp recombinase mediate genome engineering reactions in mammalian cells.Dual selection enhances the signaling specificity of a variant of the quorum-sensing transcriptional activator LuxR.
P2860
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P2860
A dual reporter screening system identifies the amino acid at position 82 in Flp site-specific recombinase as a determinant for target specificity.
description
2002 nî lūn-bûn
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2002年の論文
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2002年論文
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2002年論文
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2002年論文
@zh-hk
2002年論文
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2002年論文
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name
A dual reporter screening syst ...... minant for target specificity.
@en
A dual reporter screening syst ...... minant for target specificity.
@nl
type
label
A dual reporter screening syst ...... minant for target specificity.
@en
A dual reporter screening syst ...... minant for target specificity.
@nl
prefLabel
A dual reporter screening syst ...... minant for target specificity.
@en
A dual reporter screening syst ...... minant for target specificity.
@nl
P2093
P2860
P356
P1476
A dual reporter screening syst ...... minant for target specificity.
@en
P2093
A Francis Stewart
Makkuni Jayaram
Yuri Voziyanov
P2860
P304
P356
10.1093/NAR/30.7.1656
P407
P577
2002-04-01T00:00:00Z