Design, recombinant expression and convenient A-chain N-terminal europium-labelling of a fully active human relaxin-3 analogue.
about
The electrostatic interactions of relaxin-3 with receptor RXFP4 and the influence of its B-chain C-terminal conformationFluorescent approaches for understanding interactions of ligands with G protein coupled receptorsDevelopment of a selective agonist for relaxin family peptide receptor 3.Mechanism for insulin-like peptide 5 distinguishing the homologous relaxin family peptide receptor 3 and 4A novel BRET-based binding assay for interaction studies of relaxin family peptide receptor 3 with its ligands.Novel bioluminescent binding assays for interaction studies of protein/peptide hormones with their receptors.Bioluminescent Ligand-Receptor Binding Assays for Protein or Peptide Hormones.Secretory overexpression and isotopic labeling of the chimeric relaxin family peptide R3/I5 in Pichia pastoris.A convenient method for europium-labeling of a recombinant chimeric relaxin family peptide R3/I5 for receptor-binding assays.The highly conserved negatively charged Glu141 and Asp145 of the G-protein-coupled receptor RXFP3 interact with the highly conserved positively charged arginine residues of relaxin-3.Application of the novel bioluminescent ligand-receptor binding assay to relaxin-RXFP1 system for interaction studies.Identification of hydrophobic interactions between relaxin-3 and its receptor RXFP3: implication for a conformational change in the B-chain C-terminus during receptor binding.
P2860
Q24297116-3D4470EB-6DCA-4198-882E-C4F0FEF5B0DEQ27004213-5D28A783-67FA-4FE3-B20F-533AA63DB943Q33789534-A172B1D7-BC64-4AD6-972A-887F34C00F45Q37081723-4613FACA-F49D-491F-B24B-DF752E762CA9Q38761448-DDF9DEBC-CE2C-4723-9E25-FDD79A87E5F8Q38791521-6FAA9DA8-6E07-4DAD-AE9D-1962BF571534Q38841010-CBAACD7E-CDF8-4D49-97E3-F6AE459FD3DEQ39032858-882FFBAC-1D22-4859-BEB7-56A211061C8CQ39445866-31A3775E-3FA1-4475-83EC-A1333EFC573CQ43592780-6A980FBE-8F7E-44E8-9177-D6B5373F10BDQ51561929-5B0C47AE-83FA-479B-B467-30F3C0EF158EQ53745055-2C2441DE-BBDC-4345-997F-5413070C3212
P2860
Design, recombinant expression and convenient A-chain N-terminal europium-labelling of a fully active human relaxin-3 analogue.
description
2012 nî lūn-bûn
@nan
2012年の論文
@ja
2012年論文
@yue
2012年論文
@zh-hant
2012年論文
@zh-hk
2012年論文
@zh-mo
2012年論文
@zh-tw
2012年论文
@wuu
2012年论文
@zh
2012年论文
@zh-cn
name
Design, recombinant expression ...... tive human relaxin-3 analogue.
@en
Design, recombinant expression ...... tive human relaxin-3 analogue.
@nl
type
label
Design, recombinant expression ...... tive human relaxin-3 analogue.
@en
Design, recombinant expression ...... tive human relaxin-3 analogue.
@nl
prefLabel
Design, recombinant expression ...... tive human relaxin-3 analogue.
@en
Design, recombinant expression ...... tive human relaxin-3 analogue.
@nl
P2093
P2860
P1433
P1476
Design, recombinant expression ...... tive human relaxin-3 analogue.
@en
P2093
Wei-Jie Zhang
Xiao-Xia Shao
Xin-Yi Wang
Zhan-Yun Guo
P2860
P304
P356
10.1111/J.1742-4658.2012.08550.X
P407
P577
2012-03-21T00:00:00Z