Coamplification at lower denaturation temperature-PCR increases mutation-detection selectivity of TaqMan-based real-time PCR.
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Two-round coamplification at lower denaturation temperature-PCR (COLD-PCR)-based sanger sequencing identifies a novel spectrum of low-level mutations in lung adenocarcinomaMolecular testing guideline for selection of lung cancer patients for EGFR and ALK tyrosine kinase inhibitors: guideline from the College of American Pathologists, International Association for the Study of Lung Cancer, and Association for MolecularDISSECT Method Using PNA-LNA Clamp Improves Detection of EGFR T790m MutationCOLD-PCR-enhanced high-resolution melting enables rapid and selective identification of low-level unknown mutationsDetection of hepatitis B virus genotypic resistance mutations by coamplification at lower denaturation temperature-PCR coupled with sanger sequencing.COLD-PCR enrichment of rare cancer mutations prior to targeted amplicon resequencing.Molecular testing guideline for selection of lung cancer patients for EGFR and ALK tyrosine kinase inhibitors: guideline from the College of American Pathologists, International Association for the Study of Lung Cancer, and Association for MolecularIce-COLD-PCR enables rapid amplification and robust enrichment for low-abundance unknown DNA mutations.Enrichment of mutations in multiple DNA sequences using COLD-PCR in emulsion.Single-tube, highly parallel mutation enrichment in cancer gene panels by use of temperature-tolerant COLD-PCR.COLD-PCR: improving the sensitivity of molecular diagnostics assays.Temperature-tolerant COLD-PCR reduces temperature stringency and enables robust mutation enrichmentDifferential strand separation at critical temperature: a minimally disruptive enrichment method for low-abundance unknown DNA mutationsA sensitive and practical method to detect the T790M mutation in the epidermal growth factor receptor.The role of molecular genetic analysis within the diagnostic haemato-oncology laboratory.Biotinylated probe isolation of targeted gene region improves detection of T790M epidermal growth factor receptor mutation via peptide nucleic acid-enriched real-time PCR.COLD-PCR Technologies in the Area of Personalized Medicine: Methodology and Applications.Competitive amplification of differentially melting amplicons (CADMA) enables sensitive and direct detection of all mutation types by high-resolution melting analysis.Increased sensitivity of KRAS mutation detection by high-resolution melting analysis of COLD-PCR products.Multiplex amplification coupled with COLD-PCR and high resolution melting enables identification of low-abundance mutations in cancer samples with low DNA content.Potential clinical significance of plasma-based KRAS mutation analysis using the COLD-PCR/TaqMan(®) -MGB probe genotyping method.Detection of EGFR and BRAF mutations by competitive allele-specific TaqMan polymerase chain reaction in lung adenocarcinoma.H-ras mutation detection in bladder cancer by COLD-PCR analysis and direct sequencing.
P2860
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P2860
Coamplification at lower denaturation temperature-PCR increases mutation-detection selectivity of TaqMan-based real-time PCR.
description
2009 nî lūn-bûn
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2009年の論文
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2009年学术文章
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2009年学术文章
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2009年学术文章
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2009年学术文章
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2009年学术文章
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2009年學術文章
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2009年學術文章
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name
Coamplification at lower denat ...... of TaqMan-based real-time PCR.
@en
Coamplification at lower denat ...... of TaqMan-based real-time PCR.
@nl
type
label
Coamplification at lower denat ...... of TaqMan-based real-time PCR.
@en
Coamplification at lower denat ...... of TaqMan-based real-time PCR.
@nl
prefLabel
Coamplification at lower denat ...... of TaqMan-based real-time PCR.
@en
Coamplification at lower denat ...... of TaqMan-based real-time PCR.
@nl
P2093
P2860
P1433
P1476
Coamplification at lower denat ...... of TaqMan-based real-time PCR.
@en
P2093
G Mike Makrigiorgos
Lilin Wang
P2860
P304
P356
10.1373/CLINCHEM.2008.113381
P407
P50
P577
2009-02-20T00:00:00Z