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Standardized determination of real-time PCR efficiency from a single reaction set-up

Standardized determination of real-time PCR efficiency from a single reaction set-up

http://www.wikidata.org/entity/Q39982193

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Corticolimbic expression of TRPC4 and TRPC5 channels in the rodent brain A mechanistic model of PCR for accurate quantification of quantitative PCR data DYRK1A, a novel determinant of the methionine-homocysteine cycle in different mouse models overexpressing this Down-syndrome-associated kinase A standard curve based method for relative real time PCR data processing Neonatal local noxious insult affects gene expression in the spinal dorsal horn of adult rats Statistical analysis of real-time PCR data A method for accurate detection of genomic microdeletions using real-time quantitative PCR.Polar Fixation of Plasmids during Recombinant Protein Production in Bacillus megaterium Results in Population Heterogeneity 5-Hydroxytryptamine modulates cytokine and chemokine production in LPS-primed human monocytes via stimulation of different 5-HTR subtypes Validation of kinetics similarity in qPCR Oxidative stress and asthma: proteome analysis of chitinase-like proteins and FIZZ1 in lung tissue and bronchoalveolar lavage fluid Wnt11 promotes osteoblast maturation and mineralization through R-spondin 2 Stability of endogenous reference genes in postmortem human brains for normalization of quantitative real-time PCR data: comprehensive evaluation using geNorm, NormFinder, and BestKeeper.A new method for quantitative real-time polymerase chain reaction data analysis.No control genes required: Bayesian analysis of qRT-PCR data.qpcR: an R package for sigmoidal model selection in quantitative real-time polymerase chain reaction analysis.An improvement of the 2ˆ(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis.Simple regression for correcting ΔCt bias in RT-qPCR low-density array data normalization.Quantitative polymerase chain reaction analysis by deconvolution of internal standard.Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers Model based analysis of real-time PCR data from DNA binding dye protocols.Analysis of TaqMan Array Cards Data by an Assumption-Free Improvement of the maxRatio Algorithm Is More Accurate than the Cycle-Threshold Method.Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence.System-specific periodicity in quantitative real-time polymerase chain reaction data questions threshold-based quantitation Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetry.Evaluation of absolute quantitation by nonlinear regression in probe-based real-time PCR Systems analysis of circadian time-dependent neuronal epidermal growth factor receptor signaling.Critical points of DNA quantification by real-time PCR--effects of DNA extraction method and sample matrix on quantification of genetically modified organisms.Differential responses of Coffea arabica L. leaves and roots to chemically induced systemic acquired resistance.Particular distribution and expression pattern of endoglin (CD105) in the liver of patients with hepatocellular carcinoma.Maternal cocaine administration in mice alters DNA methylation and gene expression in hippocampal neurons of neonatal and prepubertal offspring.Sequence polymorphism can produce serious artefacts in real-time PCR assays: hard lessons from Pacific oysters.A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition.Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon.Critical evaluation of methods used to determine amplification efficiency refutes the exponential character of real-time PCR.A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance.Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data.Altered expression of microRNAs in the myocardium of rats with acute myocardial infarction.Serious overestimation in quantitative PCR by circular (supercoiled) plasmid standard: microalgal pcna as the model gene.Application of housekeeping npcRNAs for quantitative expression analysis of human transcriptome by real-time PCR.

P2860

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P2860

Standardized determination of real-time PCR efficiency from a single reaction set-up

http://www.wikidata.org/entity/Q39982193

description

2003 nî lūn-bûn

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2003年の論文

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2003年学术文章

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2003年学术文章

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2003年学术文章

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2003年学术文章

@zh-my

2003年学术文章

@zh-sg

2003年學術文章

@yue

2003年學術文章

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2003年學術文章

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name

Standardized determination of real-time PCR efficiency from a single reaction set-up

@en

Standardized determination of real-time PCR efficiency from a single reaction set-up.

@nl

type

label

Standardized determination of real-time PCR efficiency from a single reaction set-up

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Standardized determination of real-time PCR efficiency from a single reaction set-up.

@nl

prefLabel

Standardized determination of real-time PCR efficiency from a single reaction set-up

@en

Standardized determination of real-time PCR efficiency from a single reaction set-up.

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Nucleic Acids Research

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Standardized determination of real-time PCR efficiency from a single reaction set-up

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P2093

Ales Tichopad

http://www.w3.org/2001/XMLSchema#string

Gerhard Schwarz

http://www.w3.org/2001/XMLSchema#string

Michael Dilger

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P2860

A new mathematical model for relative quantification in real-time RT-PCR

Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase

Product differentiation by analysis of DNA melting curves during the polymerase chain reaction

Housekeeping genes as internal standards: use and limits

Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions

Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR

Monitoring hybridization during polymerase chain reaction.

Control selection for RNA quantitation.

Improved quantitative real-time RT-PCR for expression profiling of individual cells.

Detection of PCR products using self-probing amplicons and fluorescence.

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e122

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scholarly article

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10.1093/NAR/GNG122

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20

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31

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Michael W Pfaffl

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274589321

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