Reliable gene expression measurements from degraded RNA by quantitative real-time PCR depend on short amplicons and a proper normalization.
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PSMB2 and RPL32 are suitable denominators to normalize gene expression profiles in bronchoalveolar cellsChromatin immunoprecipitation to analyze DNA binding sites of HMGA2Severe airway epithelial injury, aberrant repair and bronchiolitis obliterans develops after diacetyl instillation in ratsQuality assessment and data handling methods for Affymetrix Gene 1.0 ST arrays with variable RNA integrity.Selecting housekeeping genes as references for the normalization of quantitative PCR data in breast cancer.Identification of genes for normalization of real-time RT-PCR data in breast carcinomas.Quantitative mRNA expression analysis of neurotrophin-receptor TrkC and oncogene c-MYC from formalin-fixed, paraffin-embedded primitive neuroectodermal tumor samples.Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells.Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT).Improved RNA quality and TaqMan Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials.Transcriptome and proteome expressions involved in insulin resistance in muscle and activated T-lymphocytes of patients with type 2 diabetes.Expression profiling with RNA from formalin-fixed, paraffin-embedded materialTissues from routine pathology archives are suitable for microRNA analyses by quantitative PCRQuantitative analysis of hTERT mRNA levels in cells microdissected from cytological specimens.Gene expression variation between distinct areas of breast cancer measured from paraffin-embedded tissue cores.Angiogenesis-related gene expression profile with independent prognostic value in advanced ovarian carcinoma.Comparison of prognostic gene profiles using qRT-PCR in paraffin samples: a retrospective study in patients with early breast cancerOptimized quantification of fragmented, free circulating DNA in human blood plasma using a calibrated duplex real-time PCR.RNA degradation compromises the reliability of microRNA expression profiling.Normalization with genes encoding ribosomal proteins but not GAPDH provides an accurate quantification of gene expressions in neuronal differentiation of PC12 cells.High-throughput quantification of splicing isoformsProspectively isolated cancer-associated CD10(+) fibroblasts have stronger interactions with CD133(+) colon cancer cells than with CD133(-) cancer cells.A multiplex endpoint RT-PCR assay for quality assessment of RNA extracted from formalin-fixed paraffin-embedded tissues.RORα and ROR γ are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20-hydroxy- and 20,23-dihydroxyvitamin D.qPCR in gastrointestinal stromal tumors: Evaluation of reference genes and expression analysis of KIT and the alternative receptor tyrosine kinases FLT3, CSF1-R, PDGFRB, MET and AXL.Assessment of fine needle aspiration feasibility and specimen adequacy for molecular diagnostics of benign vocal fold lesionsmRNA transcript quantification in archival samples using multiplexed, color-coded probes.Evaluating erythropoietin-associated tumor progression using archival tissues from a phase III clinical trial.Decrease in thyroid adenoma associated (THADA) expression is a marker of dedifferentiation of thyroid tissue.Reliable gene expression measurements from fine needle aspirates of pancreatic tumors: effect of amplicon length and quality assessment.Gene expression levels as predictive markers of outcome in pancreatic cancer after gemcitabine-based adjuvant chemotherapy.Identifying reference genes with stable expression from high throughput sequence data.Single-Tube Mutation Scanning of The Epidermal Growth Factor Receptor Gene Using Multiplex LATE-PCR and Lights-On/Lights-Off ProbesA multiplex two-color real-time PCR method for quality-controlled molecular diagnostic testing of FFPE samples.Renal tissue thawed for 30 minutes is still suitable for gene expression analysis.Paraffin embedding contributes to RNA aggregation, reduced RNA yield, and low RNA qualityDetermination of SGK1 mRNA in non-small cell lung cancer samples underlines high expression in squamous cell carcinomasAdapted approach to profile genes while reconciling Vegf-a mRNA expression in the developing and injured lung.Rates of MAGE-A3 and PRAME expressing tumors in FFPE tissue specimens from bladder cancer patients: potential targets for antigen-specific cancer immunotherapeutics.Evaluation of Existing Methods for Human Blood mRNA Isolation and Analysis for Large Studies.
P2860
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P2860
Reliable gene expression measurements from degraded RNA by quantitative real-time PCR depend on short amplicons and a proper normalization.
description
2005 nî lūn-bûn
@nan
2005年の論文
@ja
2005年論文
@yue
2005年論文
@zh-hant
2005年論文
@zh-hk
2005年論文
@zh-mo
2005年論文
@zh-tw
2005年论文
@wuu
2005年论文
@zh
2005年论文
@zh-cn
name
Reliable gene expression measu ...... ns and a proper normalization.
@en
type
label
Reliable gene expression measu ...... ns and a proper normalization.
@en
prefLabel
Reliable gene expression measu ...... ns and a proper normalization.
@en
P2093
P1476
Reliable gene expression measu ...... ns and a proper normalization.
@en
P2093
Achim Fleischmann
Andrea Oberli
Anna Baltzer
Darlene R Goldstein
Hans J Altermatt
Janine Antonov
Marco Pirotta
Rolf Jaggi
P2888
P304
P356
10.1038/LABINVEST.3700303
P577
2005-08-01T00:00:00Z