Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5' of an ATG initiation codon.
about
Identification of ribosome binding sites in Escherichia coli using neural network modelsGeneration of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coliScoring functions for computational algorithms applicable to the design of spiked oligonucleotides.C5a receptor activation. Genetic identification of critical residues in four transmembrane helices.Measurements of translation initiation from all 64 codons in E. coli.Overproduction of SecA suppresses the export defect caused by a mutation in the gene encoding the Escherichia coli export chaperone secB.Mixed oligonucleotides for random mutagenesis: best way of making them.Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes.A method for introducing random single point deletions in specific DNA target sequences using oligonucleotides.A complete library of point substitution mutations in the glucocorticoid response element of mouse mammary tumor virus.High-level expression of human superoxide dismutase in the cyanobacterium Anacystis nidulans 6301.Human Cu/Zn superoxide dismutase cDNA: isolation of clones synthesising high levels of active or inactive enzyme from an expression libraryThe highly conserved U small nuclear RNA 3'-end formation signal is quite tolerant to mutation.A general strategy for random insertion and substitution mutagenesis: substoichiometric coupling of trinucleotide phosphoramidites.Structural analysis of kasugamycin inhibition of translation.Promoters selected from random DNA sequencesOverproduction and dissection of proteins by the expression-cassette polymerase chain reaction.Gene synthesis, expression in Escherichia coli, purification and characterization of the recombinant bovine acyl-CoA-binding protein.Search for the optimal sequence of the ribosome binding site by random oligonucleotide-directed mutagenesis.Secondary structure as primary determinant of the efficiency of ribosomal binding sites in Escherichia coli.The influence of mRNA primary and secondary structure on human IFN-gamma gene expression in E. coli.Introduction of restriction enzyme sites in protein-coding DNA sequences by site-specific mutagenesis not affecting the amino acid sequence: a computer programThe use of two-cistron constructions in improving the expression of a heterologous gene in E. coli.Mutagenesis of the three bases preceding the start codon of the beta-galactosidase mRNA and its effect on translation in Escherichia coli.Sequences capable of restoring poly(A) site function define two distinct downstream elements.Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli.Quantitative Missense Variant Effect Prediction Using Large-Scale Mutagenesis Data.Construction, characterization and application of molecular tools for metabolic engineering of Synechocystis sp.
P2860
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P2860
Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5' of an ATG initiation codon.
description
1983 nî lūn-bûn
@nan
1983年の論文
@ja
1983年学术文章
@wuu
1983年学术文章
@zh-cn
1983年学术文章
@zh-hans
1983年学术文章
@zh-my
1983年学术文章
@zh-sg
1983年學術文章
@yue
1983年學術文章
@zh
1983年學術文章
@zh-hant
name
Targeted random mutagenesis: t ...... 5' of an ATG initiation codon.
@en
type
label
Targeted random mutagenesis: t ...... 5' of an ATG initiation codon.
@en
prefLabel
Targeted random mutagenesis: t ...... 5' of an ATG initiation codon.
@en
P2860
P356
P1476
Targeted random mutagenesis: t ...... 5' of an ATG initiation codon.
@en
P2093
Heyneker HL
Matteucci MD
P2860
P304
P356
10.1093/NAR/11.10.3113
P577
1983-05-01T00:00:00Z