Site-specific mutagenesis using asymmetric polymerase chain reaction and a single mutant primer.
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Leukotriene A4 hydrolase: determination of the three zinc-binding ligands by site-directed mutagenesis and zinc analysisExpression and biological activity of the cystine knot bioinsecticide PA1b (Pea Albumin 1 Subunit b)Regulation of expression of the rat SOCS-3 gene in hepatocytes by growth hormone, interleukin-6 and glucocorticoids mRNA analysis and promoter characterizationFiber-type-specific transcription of the troponin I slow gene is regulated by multiple elementsAntigenic diversity among Helicobacter pylori vacuolating toxinsA DNA unwinding element and an ARS consensus comprise a replication origin within a yeast chromosome.Identification of a cytoplasm to vacuole targeting determinant in aminopeptidase IAn AP-1 binding sequence is essential for regulation of the human alpha2(I) collagen (COL1A2) promoter activity by transforming growth factor-beta.Multiple DNA elements in ARS305 determine replication origin activity in a yeast chromosome.Oncogene activation of HIV-LTR-driven expression via the NF-kappa B binding sitesLocalization of biologically important regions on toxic shock syndrome toxin 1.Analysis of toxicity of streptococcal pyrogenic exotoxin A mutantsProbing the structure, function, and interactions of the Escherichia coli H-NS and StpA proteins by using dominant negative derivatives.The phylogenetically conserved doublet tertiary interaction in domain III of the large subunit rRNA is crucial for ribosomal protein bindingAgrobacterium transcriptional regulator Ros is a prokaryotic zinc finger protein that regulates the plant oncogene iptTacaribe virus Z protein interacts with the L polymerase protein to inhibit viral RNA synthesis.Transcription of the dominant-negative helix-loop-helix protein Id1 is regulated by a protein complex containing the immediate-early response gene Egr-1.Functional equivalency and diversity of cis-acting elements among yeast replication origins.Transcriptional activation of several heterologous promoters by the E6 protein of human papillomavirus type 16.Mouse retinoic acid receptor alpha 2 isoform is transcribed from a promoter that contains a retinoic acid response element.Dimerization interfaces formed between the DNA binding domains determine the cooperative binding of RXR/RAR and RXR/TR heterodimers to DR5 and DR4 elements.C/EBP proteins activate transcription from the human immunodeficiency virus type 1 long terminal repeat in macrophages/monocytesGATA elements are necessary for the activity and tissue specificity of the T-cell receptor beta-chain transcriptional enhancer.Autoregulation of the Escherichia coli melR promoter: repression involves four molecules of MelR.Reaction mechanism of recombinant 3-oxoacyl-(acyl-carrier-protein) synthase III from Cuphea wrightii embryo, a fatty acid synthase type II condensing enzyme.Functional interchangeability of TFIIIB components from yeast and human cells in vitro.Expression, purification, and mechanistic studies of bovine mitochondrial translational initiation factor 2.Identification of an essential region for internal initiation of translation in the aphthovirus internal ribosome entry site and implications for viral evolutionDevelopment of an inducible pol III transcription system essentially requiring a mutated form of the TATA-binding protein.Genetic variation and RNA structure regulate microRNA biogenesis.A viral nonstructural protein regulates bluetongue virus trafficking and release.Amino-terminal hydrophobic region of Helicobacter pylori vacuolating cytotoxin (VacA) mediates transmembrane protein dimerization.Characterization of the helper proteins for the assembly of tail fibers of coliphages T4 and lambdaHomomeric interactions between transmembrane proteins of Moloney murine leukemia virus.Hierarchy and positive/negative interplays of the hepatocyte nuclear factors HNF-1, -3 and -4 in the liver-specific enhancer for the human alpha-1-microglobulin/bikunin precursor.An efficient 1-tube PCR method for internal site-directed mutagenesis of large amplified moleculesComputing exponentially faster: implementing a non-deterministic universal Turing machine using DNA.An efficient system for the generation of marked genetic mutants in members of the genus Burkholderia.Identification and analysis of a retinoblastoma binding motif in the replication protein of a plant DNA virus: requirement for efficient viral DNA replication.Defining the enzyme binding domain of a ribonuclease III processing signal. Ethylation interference and hydroxyl radical footprinting using catalytically inactive RNase III mutants.
P2860
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P2860
Site-specific mutagenesis using asymmetric polymerase chain reaction and a single mutant primer.
description
1990 nî lūn-bûn
@nan
1990年の論文
@ja
1990年論文
@yue
1990年論文
@zh-hant
1990年論文
@zh-hk
1990年論文
@zh-mo
1990年論文
@zh-tw
1990年论文
@wuu
1990年论文
@zh
1990年论文
@zh-cn
name
Site-specific mutagenesis usin ...... on and a single mutant primer.
@en
type
label
Site-specific mutagenesis usin ...... on and a single mutant primer.
@en
prefLabel
Site-specific mutagenesis usin ...... on and a single mutant primer.
@en
P356
P1476
Site-specific mutagenesis usin ...... on and a single mutant primer.
@en
P2093
P2860
P304
P356
10.1093/NAR/18.24.7433
P577
1990-12-01T00:00:00Z