Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution.
about
Protein engineering and its applications in food industry.Structural and catalytic response to temperature and cosolvents of carboxylesterase EST1 from the extremely thermoacidophilic archaeon Sulfolobus solfataricus P1.Laboratory-directed protein evolutionDirected evolution: an approach to engineer enzymes.Rational Engineering of a Cold-Adapted α-Amylase from the Antarctic Ciliate Euplotes focardii for Simultaneous Improvement of Thermostability and Catalytic Activity.Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution.Discovery of superior enzymes by directed molecular evolution.Genomic and exoproteomic analyses of cold- and alkaline-adapted bacteria reveal an abundance of secreted subtilisin-like proteases.Crystallization and preliminary X-ray crystallographic studies of a psychrophilic subtilisin-like protease Apa1 from Antarctic Pseudoalteromonas sp. strain AS-11Improving the Thermostability and Activity of a Thermophilic Subtilase by Incorporating Structural Elements of Its Psychrophilic Counterpart.Molecular Structural Basis for the Cold Adaptedness of the Psychrophilic β-Glucosidase BglU in Micrococcus antarcticus.Cold adaptation of zinc metalloproteases in the thermolysin family from deep sea and arctic sea ice bacteria revealed by catalytic and structural properties and molecular dynamics: new insights into relationship between conformational flexibility anPsychrophilic enzymes: from folding to function and biotechnology.Proteases from psychrotrophs: an overview.Microbial proteases: detection, production, and genetic improvement.Cellulases from psychrophilic microorganisms: a review.Biotechnology of cold-active proteases.Generation and analysis of mesophilic variants of the thermostable archaeal I-DmoI homing endonuclease.Metabolic enzymes from psychrophilic bacteria: challenge of adaptation to low temperatures in ornithine carbamoyltransferase from Moritella abyssi.Single amino acid mutation alters thermostability of the alkaline protease from Bacillus pumilus: thermodynamics and temperature dependence.Thermodynamic stability of a cold-adapted protein, type III antifreeze protein, and energetic contribution of salt bridges.Directed evolution of a bacterial alpha-amylase: toward enhanced pH-performance and higher specific activity.Site-directed mutagenesis of a family 42 β-galactosidase from an antarctic bacterium.Different roles of two gamma-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii: remodelling of interaction surfaces may enhance microtubule nucleation at low temperature.Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae.Cold-adapted maturation of thermophilic WF146 protease by mimicking the propeptide binding interactions of psychrophilic subtilisin S41.Residues at the active site of the esterase 2 from Alicyclobacillus acidocaldarius involved in substrate specificity and catalytic activity at high temperature.Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species.Structural and functional adaptations to extreme temperatures in psychrophilic, mesophilic, and thermophilic DNA ligases.Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis. Gene cloning and protein characterization.Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase.Unscrambling thermal stability and temperature adaptation in evolved variants of a cold-active lipase.Cloning, expression and decoding of the cold adaptation of a new widely represented thermolabile subtilisin-like protease.Determinants of cardiac Na(+)/Ca(2+) exchanger temperature dependence: NH(2)-terminal transmembrane segments.Single-site substitutions improve cold activity and increase thermostability of the dehairing alkaline protease (DHAP).Engineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution.
P2860
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P2860
Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution.
description
2000 nî lūn-bûn
@nan
2000年の論文
@ja
2000年論文
@yue
2000年論文
@zh-hant
2000年論文
@zh-hk
2000年論文
@zh-mo
2000年論文
@zh-tw
2000年论文
@wuu
2000年论文
@zh
2000年论文
@zh-cn
name
Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution.
@en
type
label
Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution.
@en
prefLabel
Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution.
@en
P2093
P2860
P356
P1476
Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution.
@en
P2093
Miyazaki K
Wintrode PL
P2860
P304
31635-31640
P356
10.1074/JBC.M004503200
P407
P577
2000-10-01T00:00:00Z