Quantification of the detrimental effect of a single primer-template mismatch by real-time PCR using the 16S rRNA gene as an example.
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A comparative study of microbial diversity and community structure in marine sediments using poly(A) tailing and reverse transcription-PCRDistribution of high bacterial taxa across the chronosequence of two alpine glacier forelands.Exploring the composition and diversity of microbial communities at the Jan Mayen hydrothermal vent field using RNA and DNA.Development of a prokaryotic universal primer for simultaneous analysis of Bacteria and Archaea using next-generation sequencingPCR primers for metazoan mitochondrial 12S ribosomal DNA sequencesCoverage evaluation of universal bacterial primers using the metagenomic datasets.High-throughput sequencing of 16S rRNA gene amplicons: effects of extraction procedure, primer length and annealing temperatureSeasonal diversity of planktonic protists in Southwestern Alberta rivers over a 1-year period as revealed by terminal restriction fragment length polymorphism and 18S rRNA gene library analyses.DNA metabarcoding and the cytochrome c oxidase subunit I marker: not a perfect match.A comprehensive evaluation of PCR primers to amplify the nifH gene of nitrogenase.Groundtruthing next-gen sequencing for microbial ecology-biases and errors in community structure estimates from PCR amplicon pyrosequencingDiversity of phytoplankton nitrate transporter sequences from isolated single cells and mixed samples from the East China Sea and mRNA quantification.Comparison of bacterial culture and 16S rRNA community profiling by clonal analysis and pyrosequencing for the characterization of the dentine caries-associated microbiome.Evaluation of four endogenous reference genes and their real-time PCR assays for common wheat quantification in GMOs detectionEvidence for the importance of litter as a co-substrate for MCPA dissipation in an agricultural soil.Deconstructing the polymerase chain reaction: understanding and correcting bias associated with primer degeneracies and primer-template mismatches.Simple absolute quantification method correcting for quantitative PCR efficiency variations for microbial community samples.PCR Strategies for Complete Allele Calling in Multigene Families Using High-Throughput Sequencing Approaches.Uncovering Trophic Interactions in Arthropod Predators through DNA Shotgun-Sequencing of Gut Contents.MIPE: A metagenome-based community structure explorer and SSU primer evaluation toolDNA extraction replicates improve diversity and compositional dissimilarity in metabarcoding of eukaryotes in marine sediments.Assessing vertebrate biodiversity in a kelp forest ecosystem using environmental DNA.Resolving rates of mutation in the brain using single-neuron genomicsCell-associated HIV RNA: a dynamic biomarker of viral persistence.Ecotoxicological Impact of the Bioherbicide Leptospermone on the Microbial Community of Two Arable SoilsEvaluation of single-nucleotide primer extension for detection and typing of phylogenetic markers used for investigation of microbial communities.Design and Evaluation of Illumina MiSeq-Compatible, 18S rRNA Gene-Specific Primers for Improved Characterization of Mixed Phototrophic Communities.Comparison of primer sets for the study of Planctomycetes communities in lentic freshwater ecosystems.Differential haplotype amplification leads to misgenotyping of heterozygote as homozygote when using single nucleotide mismatch primer.Degenerate ITS7 primer enhances oomycete community coverage and PCR sensitivity to Aphanomyces species, economically important plant pathogens.Experimental warming effects on the microbial community of a temperate mountain forest soil.Ecological differentiation in planktonic and sediment-associated chemotrophic microbial populations in Yellowstone hot springs.Vector soup: high-throughput identification of Neotropical phlebotomine sand flies using metabarcoding.Reoccurrence of botulinum neurotoxin subtype A3 inducing food-borne botulism, Slovakia, 2015.Real-time PCR assay for distinguishing Frankliniella occidentalis and Thrips palmi Arnika Przybylska, Żaneta Fiedler, Aleksandra Obrępalska-Stęplowska.454-Pyrosequencing Analysis of Bacterial Communities from Autotrophic Nitrogen Removal Bioreactors Utilizing Universal Primers: Effect of Annealing Temperature.Differentiation of entC1 from entC2/entC3 with a single primer pair using simple and rapid SYBR Green-based RT-PCR melt curve analysis.Underestimation of ammonia-oxidizing bacteria abundance by amplification bias in amoA-targeted qPCR.Selectivity of Enzymatic Conversion of Oligonucleotide Probes during Nucleotide Polymorphism Analysis of DNA.The effect of primer-template mismatches on the detection and quantification of nucleic acids using the 5' nuclease assay.
P2860
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P2860
Quantification of the detrimental effect of a single primer-template mismatch by real-time PCR using the 16S rRNA gene as an example.
description
2008 nî lūn-bûn
@nan
2008年の論文
@ja
2008年学术文章
@wuu
2008年学术文章
@zh
2008年学术文章
@zh-cn
2008年学术文章
@zh-hans
2008年学术文章
@zh-my
2008年学术文章
@zh-sg
2008年學術文章
@yue
2008年學術文章
@zh-hant
name
Quantification of the detrimen ...... e 16S rRNA gene as an example.
@en
Quantification of the detrimen ...... e 16S rRNA gene as an example.
@nl
type
label
Quantification of the detrimen ...... e 16S rRNA gene as an example.
@en
Quantification of the detrimen ...... e 16S rRNA gene as an example.
@nl
prefLabel
Quantification of the detrimen ...... e 16S rRNA gene as an example.
@en
Quantification of the detrimen ...... e 16S rRNA gene as an example.
@nl
P2860
P356
P1476
Quantification of the detrimen ...... he 16S rRNA gene as an example
@en
P2093
P2860
P304
P356
10.1128/AEM.02403-07
P407
P577
2008-01-11T00:00:00Z