Hydrophobic amino acid residues in the acceptor binding site are main determinants for reaction mechanism and specificity of cyclodextrin-glycosyltransferase.
about
Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteriaThe remote substrate binding subsite -6 in cyclodextrin-glycosyltransferase controls the transferase activity of the enzyme via an induced-fit mechanismRole of Phe283 in enzymatic reaction of cyclodextrin glycosyltransferase from alkalophilic Bacillus sp.1011: Substrate binding and arrangement of the catalytic sitePurification, characterization, and gene cloning of a novel maltosyltransferase from an Arthrobacter globiformis strain that produces an alternating alpha-1,4- and alpha-1,6-cyclic tetrasaccharide from starchFusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donorPhosphorylation and sulfation of oligosaccharide substrates critically influence the activity of human beta1,4-galactosyltransferase 7 (GalT-I) and beta1,3-glucuronosyltransferase I (GlcAT-I) involved in the biosynthesis of the glycosaminoglycan-proSystems engineering of tyrosine 195, tyrosine 260, and glutamine 265 in cyclodextrin glycosyltransferase from Paenibacillus macerans to enhance maltodextrin specificity for 2-O-(D)-glucopyranosyl-(L)-ascorbic acid synthesisEnhancing the α-Cyclodextrin Specificity of Cyclodextrin Glycosyltransferase from Paenibacillus macerans by Mutagenesis Masking Subsite -7The evolution of cyclodextrin glucanotransferase product specificity.Iterative saturation mutagenesis of -6 subsite residues in cyclodextrin glycosyltransferase from Paenibacillus macerans to improve maltodextrin specificity for 2-O-D-glucopyranosyl-L-ascorbic acid synthesis.Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications.Enzymatic glycosylation of small molecules: challenging substrates require tailored catalysts.Evolution toward small molecule inhibitor resistance affects native enzyme function and stability, generating acarbose-insensitive cyclodextrin glucanotransferase variants.Unraveling the difference between invertases and fructan exohydrolases: a single amino acid (Asp-239) substitution transforms Arabidopsis cell wall invertase1 into a fructan 1-exohydrolase.Characterization and gene cloning of a maltotriose-forming exo-amylase from Kitasatospora sp. MK-1785.4,6-α-Glucanotransferase activity occurs more widespread in Lactobacillus strains and constitutes a separate GH70 subfamilySingle amino acid residue changes in subsite -1 of levansucrase from Zymomonas mobilis 10232 strongly influence the enzyme activities and products.Tyrosine 105 and threonine 212 at outermost substrate binding subsites -6 and +4 control substrate specificity, oligosaccharide cleavage patterns, and multiple binding modes of barley alpha-amylase 1.Glycosynthesis in a waterworld: new insight into the molecular basis of transglycosylation in retaining glycoside hydrolases.Comparison of lipases and glycoside hydrolases as catalysts in synthesis reactions.Comparative Analysis of the Molecular Adjuvants and Their Binding Efficiency with CR1.Stepwise error-prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus spStructural determinants allowing transferase activity in SENSITIVE TO FREEZING 2, classified as a family I glycosyl hydrolase.Identification of the sequence motif of glycoside hydrolase 13 family members.The cyclodextrin glycosyltransferase of Paenibacillus pabuli US132 strain: molecular characterization and overproduction of the recombinant enzyme.Enhancement of the alcoholytic activity of alpha-amylase AmyA from Thermotoga maritima MSB8 (DSM 3109) by site-directed mutagenesis.Site-saturation engineering of lysine 47 in cyclodextrin glycosyltransferase from Paenibacillus macerans to enhance substrate specificity towards maltodextrin for enzymatic synthesis of 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G).Decreasing the sialidase activity of multifunctional Pasteurella multocida α2-3-sialyltransferase 1 (PmST1) by site-directed mutagenesis.β-cyclodextrin production by the cyclodextrin glucanotransferase from Paenibacillus illinoisensis ZY-08: cloning, purification, and properties.Mutations converting cyclodextrin glycosyltransferase from a transglycosylase into a starch hydrolase.Increased amylosucrase activity and specificity, and identification of regions important for activity, specificity and stability through molecular evolution.Engineering of isoamylase: improvement of protein stability and catalytic efficiency through semi-rational design.Comparative analysis of the molecular adjuvants and their binding efficiency with CR1.
P2860
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P2860
Hydrophobic amino acid residues in the acceptor binding site are main determinants for reaction mechanism and specificity of cyclodextrin-glycosyltransferase.
description
2001 nî lūn-bûn
@nan
2001年の論文
@ja
2001年学术文章
@wuu
2001年学术文章
@zh-cn
2001年学术文章
@zh-hans
2001年学术文章
@zh-my
2001年学术文章
@zh-sg
2001年學術文章
@yue
2001年學術文章
@zh
2001年學術文章
@zh-hant
name
Hydrophobic amino acid residue ...... lodextrin-glycosyltransferase.
@en
Hydrophobic amino acid residue ...... lodextrin-glycosyltransferase.
@nl
type
label
Hydrophobic amino acid residue ...... lodextrin-glycosyltransferase.
@en
Hydrophobic amino acid residue ...... lodextrin-glycosyltransferase.
@nl
prefLabel
Hydrophobic amino acid residue ...... lodextrin-glycosyltransferase.
@en
Hydrophobic amino acid residue ...... lodextrin-glycosyltransferase.
@nl
P2093
P2860
P356
P1476
Hydrophobic amino acid residue ...... clodextrin-glycosyltransferase
@en
P2093
Dijkstra BW
Leemhuis H
Uitdehaag JC
van der Veen BA
P2860
P304
44557-44562
P356
10.1074/JBC.M107533200
P407
P577
2001-09-12T00:00:00Z