Development and characterization of a series of soluble tetrameric and monomeric streptavidin muteins with differential biotin binding affinities.
about
Avidin related protein 2 shows unique structural and functional features among the avidin protein family.Tamavidins--novel avidin-like biotin-binding proteins from the Tamogitake mushroomEvolved streptavidin mutants reveal key role of loop residue in high-affinity bindingThe structure of the SBP-Tag-streptavidin complex reveals a novel helical scaffold bridging binding pockets on separate subunits.Development of a Tetrameric Streptavidin Mutein with Reversible Biotin Binding Capability: Engineering a Mobile Loop as an Exit Door for BiotinZebavidin - An Avidin-Like Protein from ZebrafishNanobody-coupled microbubbles as novel molecular tracerEngineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluoresceinEngineering soluble monomeric streptavidin with reversible biotin binding capability.Structure-guided design of an engineered streptavidin with reusability to purify streptavidin-binding peptide tagged proteins or biotinylated proteins.DNA nanoswitches: a quantitative platform for gel-based biomolecular interaction analysisEngineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol CouplingSingle molecule enzyme-linked immunosorbent assays: theoretical considerationsConstraints on voltage sensor movement in the shaker K+ channelDevelopment of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.Cooperative hydrogen bond interactions in the streptavidin-biotin systemExtending Bell's model: how force transducer stiffness alters measured unbinding forces and kinetics of molecular complexes.A specific aptamer-cell penetrating peptides complex delivered siRNA efficiently and suppressed prostate tumor growth in vivo.Directed evolution of streptavidin variants using in vitro compartmentalization.Association kinetics from single molecule force spectroscopy measurements.Quantification of specific bindings of biomolecules by magnetorelaxometry.Cell-permeable capsids as universal antigen carrier for the induction of an antigen-specific CD8+ T-cell response.A simple approach for preparation of affinity matrices: Simultaneous purification and reversible immobilization of a streptavidin mutein to agarose matrix.Quadruplex-and-Mg(2+) Connection (QMC) of DNA.Rapid oxime and hydrazone ligations with aromatic aldehydes for biomolecular labeling.Mutation of the important Tyr-33 residue of chicken avidin: functional and structural consequencesProtein unfolding with a steric trap.Actively transporting virus like analytes with optofluidics for rapid and ultrasensitive biodetection.Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli.Rational design of an active avidin monomer.Chicken avidin-related protein 4/5 shows superior thermal stability when compared with avidin while retaining high affinity to biotin.Construction of a dual chain pseudotetrameric chicken avidin by combining two circularly permuted avidins.Elastin-like polypeptide switches: A design strategy to detect multimeric proteins.Purification of CD47-streptavidin fusion protein from bacterial lysate using biotin-agarose affinity chromatography.Streptavidin conjugation and quantification-a method evaluation for nanoparticles.
P2860
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P2860
Development and characterization of a series of soluble tetrameric and monomeric streptavidin muteins with differential biotin binding affinities.
description
2001 nî lūn-bûn
@nan
2001年の論文
@ja
2001年学术文章
@wuu
2001年学术文章
@zh-cn
2001年学术文章
@zh-hans
2001年学术文章
@zh-my
2001年学术文章
@zh-sg
2001年學術文章
@yue
2001年學術文章
@zh
2001年學術文章
@zh-hant
name
Development and characterizati ...... ial biotin binding affinities.
@en
Development and characterizati ...... ial biotin binding affinities.
@nl
type
label
Development and characterizati ...... ial biotin binding affinities.
@en
Development and characterizati ...... ial biotin binding affinities.
@nl
prefLabel
Development and characterizati ...... ial biotin binding affinities.
@en
Development and characterizati ...... ial biotin binding affinities.
@nl
P2093
P2860
P356
P1476
Development and characterizati ...... ial biotin binding affinities.
@en
P2093
P2860
P304
46422-46428
P356
10.1074/JBC.M107398200
P407
P577
2001-10-02T00:00:00Z