Three-dimensional structural dynamics of myosin V by single-molecule fluorescence polarization.
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A unique ATP hydrolysis mechanism of single-headed processive myosin, myosin IXThree myosin V structures delineate essential features of chemo-mechanical transductionRegulation of the Postsynaptic Compartment of Excitatory Synapses by the Actin Cytoskeleton in Health and Its Disruption in DiseaseNano-scale measurement of biomolecules by optical microscopy and semiconductor nanoparticlesFilming biomolecular processes by high-speed atomic force microscopyActin structure-dependent stepping of myosin 5a and 10 during processive movementOptimized measurements of separations and angles between intra-molecular fluorescent markers.Allosteric communication in myosin V: from small conformational changes to large directed movementsExperimental test of connector rotation during DNA packaging into bacteriophage phi29 capsidsKinetic mechanism of myosin IXB and the contributions of two class IX-specific regionsLever-arm mechanics of processive myosinsEffects of surface tethering on protein folding mechanisms.Toward protein structure in situ: comparison of two bifunctional rhodamine adducts of troponin C.Fluorescence applications in molecular neurobiologySingle molecule optical measurements of orientation and rotations of biological macromoleculesSingle cell optical imaging and spectroscopy.Unconstrained steps of myosin VI appear longest among known molecular motors.Measurement of single macromolecule orientation by total internal reflection fluorescence polarization microscopy.Adaptability of myosin V studied by simultaneous detection of position and orientation.Defocused orientation and position imaging (DOPI) of myosin V.Twirling of actin by myosins II and V observed via polarized TIRF in a modified gliding assay.Mechanochemical model for myosin V.Myosin VI undergoes a 180 degrees power stroke implying an uncoupling of the front lever arm.Single-molecule stepping and structural dynamics of myosin X.Head of myosin IX binds calmodulin and moves processively toward the plus-end of actin filaments.Optimized localization analysis for single-molecule tracking and super-resolution microscopy.Full-length myosin Va exhibits altered gating during processive movement on actin.Design principles governing the motility of myosin V.A programmable DNA origami nanospring that reveals force-induced adjacent binding of myosin VI headsSingle molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time.Extracting dwell time sequences from processive molecular motor dataDifferential labeling of myosin V heads with quantum dots allows direct visualization of hand-over-hand processivitySpatially and temporally synchronized atomic force and total internal reflection fluorescence microscopy for imaging and manipulating cells and biomolecules.Functional studies of individual myosin molecules.The motor mechanism of myosin V: insights for muscle contractionImpact of emission anisotropy on fluorescence spectroscopy and FRET distance measurementsPlasmonic nanorod absorbers as orientation sensors.Structure and dynamics of the kinesin-microtubule interaction revealed by fluorescence polarization microscopy.Coupling of two non-processive myosin 5c dimers enables processive stepping along actin filaments.Influence of lever structure on myosin 5a walking
P2860
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P2860
Three-dimensional structural dynamics of myosin V by single-molecule fluorescence polarization.
description
2003 nî lūn-bûn
@nan
2003年の論文
@ja
2003年学术文章
@wuu
2003年学术文章
@zh
2003年学术文章
@zh-cn
2003年学术文章
@zh-hans
2003年学术文章
@zh-my
2003年学术文章
@zh-sg
2003年學術文章
@yue
2003年學術文章
@zh-hant
name
Three-dimensional structural d ...... ule fluorescence polarization.
@en
Three-dimensional structural d ...... ule fluorescence polarization.
@nl
type
label
Three-dimensional structural d ...... ule fluorescence polarization.
@en
Three-dimensional structural d ...... ule fluorescence polarization.
@nl
prefLabel
Three-dimensional structural d ...... ule fluorescence polarization.
@en
Three-dimensional structural d ...... ule fluorescence polarization.
@nl
P2093
P2860
P356
P1433
P1476
Three-dimensional structural d ...... ule fluorescence polarization.
@en
P2093
John E T Corrie
Joseph N Forkey
M Alexander Shaw
Margot E Quinlan
Yale E Goldman
P2860
P2888
P304
P356
10.1038/NATURE01529
P407
P577
2003-03-01T00:00:00Z
P5875
P6179
1003160992