Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.
about
Parallel in vivo DNA assembly by recombination: experimental demonstration and theoretical approachesTwo more pieces of the colibactin genotoxin puzzle from Escherichia coli show incorporation of an unusual 1-aminocyclopropanecarboxylic acid moiety.Heterologous expression of pikromycin biosynthetic gene cluster using Streptomyces artificial chromosome system.An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial speciesManipulating natural product biosynthetic pathways via DNA assembler.Mining the metabiome: identifying novel natural products from microbial communities.Direct cloning, genetic engineering, and heterologous expression of the syringolin biosynthetic gene cluster in E. coli through Red/ET recombineering.iBrick: a new standard for iterative assembly of biological parts with homing endonucleases.Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleases.Genome engineering and direct cloning of antibiotic gene clusters via phage ϕBT1 integrase-mediated site-specific recombination in Streptomyces.Overview of post Cohen-Boyer methods for single segment cloning and for multisegment DNA assembly.DNA fragments assembly based on nicking enzyme system.Recent advances in DNA assembly technologiesDNA assembly techniques for next-generation combinatorial biosynthesis of natural products.Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold.Subcloning plus insertion (SPI)--a novel recombineering method for the rapid construction of gene targeting vectorsStrain-level diversity of secondary metabolism in Streptomyces albusThe distribution pattern of DNA and protoxin in Bacillus thuringiensis as revealed by laser confocal microscopy analysis.Lambda red mediated gap repair utilizes a novel replicative intermediate in Escherichia coliDifferential requirements of singleplex and multiplex recombineering of large DNA constructs.Rapid prototyping of microbial cell factories via genome-scale engineering.A Single-Strand Annealing Protein Clamps DNA to Detect and Secure HomologyCloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies.Allopatric integrations selectively change host transcriptomes, leading to varied expression efficiencies of exotic genes in Myxococcus xanthus.Genetic engineering and heterologous expression of the disorazol biosynthetic gene cluster via Red/ET recombineering.Room temperature electrocompetent bacterial cells improve DNA transformation and recombineering efficiency.Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters.Identification of antibacterial peptides from endophytic microbiome.Using natural products for drug discovery: the impact of the genomics era.In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing.New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters.Reinvigorating natural product combinatorial biosynthesis with synthetic biologyIdentification of Thiotetronic Acid Antibiotic Biosynthetic Pathways by Target-directed Genome MiningProduction of cytotoxic glidobactins/luminmycins by Photorhabdus asymbiotica in liquid media and live cricketsA Tet-on and Cre-loxP Based Genetic Engineering System for Convenient Recycling of Selection Markers in Penicillium oxalicum."Cre/loxP plus BAC": a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens.Molecular tools for chemical biotechnology.An improved method for oriT-directed cloning and functionalization of large bacterial genomic regions.Functional Genome Mining for Metabolites Encoded by Large Gene Clusters through Heterologous Expression of a Whole-Genome Bacterial Artificial Chromosome Library in Streptomyces spp.Simple and rapid direct cloning and heterologous expression of natural product biosynthetic gene cluster in Bacillus subtilis via Red/ET recombineering.
P2860
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P2860
Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.
description
2012 nî lūn-bûn
@nan
2012年の論文
@ja
2012年学术文章
@wuu
2012年学术文章
@zh
2012年学术文章
@zh-cn
2012年学术文章
@zh-hans
2012年学术文章
@zh-my
2012年学术文章
@zh-sg
2012年學術文章
@yue
2012年學術文章
@zh-hant
name
Full-length RecE enhances line ...... ct cloning for bioprospecting.
@en
Full-length RecE enhances line ...... ct cloning for bioprospecting.
@nl
type
label
Full-length RecE enhances line ...... ct cloning for bioprospecting.
@en
Full-length RecE enhances line ...... ct cloning for bioprospecting.
@nl
prefLabel
Full-length RecE enhances line ...... ct cloning for bioprospecting.
@en
Full-length RecE enhances line ...... ct cloning for bioprospecting.
@nl
P2093
P2860
P356
P1433
P1476
Full-length RecE enhances line ...... ect cloning for bioprospecting
@en
P2093
A Francis Stewart
Alberto Plaza
Hailong Wang
Philipp M Seibert
Shengbaio Hu
Youming Zhang
P2860
P2888
P304
P356
10.1038/NBT.2183
P577
2012-05-01T00:00:00Z
P5875
P6179
1044649672