about
Multiple elements in the eIF4G1 N-terminus promote assembly of eIF4G1•PABP mRNPs in vivo.Yeast eukaryotic initiation factor 4B (eIF4B) enhances complex assembly between eIF4A and eIF4G in vivoYeast eIF4B binds to the head of the 40S ribosomal subunit and promotes mRNA recruitment through its N-terminal and internal repeat domainsThe 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to stimulate canonical translation initiation and to block an alternative pathway.Preparation and evaluation of acylated tRNAs.Role of hybrid tRNA-binding states in ribosomal translocation.Destabilization of the P site codon-anticodon helix results from movement of tRNA into the P/E hybrid state within the ribosome.Reverse translocation of tRNA in the ribosome.Recognition and positioning of mRNA in the ribosome by tRNAs with expanded anticodons.Reverse transcriptase dideoxy sequencing of RNA.Sanger dideoxy sequencing of DNA.Identification and characterization of functionally critical, conserved motifs in the internal repeats and N-terminal domain of yeast translation initiation factor 4B (yeIF4B).CRISPR Gene Editing in Yeast: An Experimental Protocol for an Upper-Division Undergraduate Laboratory CourseRNA Purification – Precipitation MethodsReconstitution and analyses of RNA interactions with eukaryotic translation initiation factors and ribosomal preinitiation complexes
P50
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P50
description
hulumtuese
@sq
researcher
@en
wetenschapper
@nl
հետազոտող
@hy
name
Sarah E. Walker
@ast
Sarah E. Walker
@en
Sarah E. Walker
@es
Sarah E. Walker
@nl
Sarah E. Walker
@sl
type
label
Sarah E. Walker
@ast
Sarah E. Walker
@en
Sarah E. Walker
@es
Sarah E. Walker
@nl
Sarah E. Walker
@sl
prefLabel
Sarah E. Walker
@ast
Sarah E. Walker
@en
Sarah E. Walker
@es
Sarah E. Walker
@nl
Sarah E. Walker
@sl
P106
P1153
9332661400
P21
P31
P4012
P496
0000-0002-9983-2485