about
Use of ImageJ to recover information from individual cells in a G protein-coupled receptor assayDynamic binding capacity of plasmid DNA in histidine-agarose chromatography.Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification.Purification of plasmids for gene therapy and DNA vaccination.Horseradish peroxidase: a valuable tool in biotechnology.Trends in dengue diagnosis.Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila StCBM64C.Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA.Structural instability of plasmid biopharmaceuticals: challenges and implications.DNA vaccines: a rational design against parasitic diseases.Mutation detection in plasmid-based biopharmaceuticals.Towards the miniaturization of GPCR-based live-cell screening assays.G protein-coupled receptors: an overview of signaling mechanisms and screening assays.Towards effective non-viral gene delivery vector.Trypanosoma brucei: immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis.Engineering of Escherichia coli strains for plasmid biopharmaceutical production: scale-up challenges.Translational features of human alpha 2b interferon production in Escherichia coli.Monitoring intracellular calcium in response to GPCR activation using thin-film silicon photodiodes with integrated fluorescence filters.Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis.Evaluation of the effect of non-B DNA structures on plasmid integrity via accelerated stability studies.Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition.Capture and detection of DNA hybrids on paper via the anchoring of antibodies with fusions of carbohydrate binding modules and ZZ-domains.Evidence that the insertion events of IS2 transposition are biased towards abrupt compositional shifts in target DNA and modulated by a diverse set of culture parameters.Enhancement of DNA vaccine efficacy by intracellular targeting strategies.Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography.A multiphasic hollow fiber reactor for the whole-cell bioconversion of 2-methyl-1,3-propanediol to (r)-beta-hydroxyisobutyric acid.Production, purification and analysis of an experimental DNA vaccine against rabies.Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B. Application of the solvophobic theory.Impact of plasmid quality on lipoplex-mediated transfection.Alternatives for the intermediate recovery of plasmid DNA: performance, economic viability and environmental impact.Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus.Development of a candidate DNA vaccine against Maedi-Visna virus.Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: fermentation strategies and impact on downstream processing.Anion exchange purification of plasmid DNA using expanded bed adsorption.On the stability of plasmid DNA vectors during cell culture and purification.Development of a recombinant fusion protein based on the dynein light chain LC8 for non-viral gene delivery.Chromatography of plasmid DNA.Development of a nicking endonuclease-assisted method for the purification of minicircles.Separation of plasmid DNA topoisomers by multimodal chromatography.Improvement of DNA minicircle production by optimization of the secondary structure of the 5'-UTR of ParA resolvase.
P50
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P50
description
hulumtues
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onderzoeker
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հետազոտող
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Duarte Miguel F Prazeres
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Duarte Miguel F Prazeres
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Duarte Miguel F Prazeres
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Duarte Miguel F Prazeres
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Duarte Miguel F Prazeres
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type
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Duarte Miguel F Prazeres
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Duarte Miguel F Prazeres
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Duarte Miguel F Prazeres
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Duarte Miguel F Prazeres
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Duarte Miguel F Prazeres
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Duarte Miguel F Prazeres
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Duarte Miguel F Prazeres
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Duarte Miguel F Prazeres
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Duarte Miguel F Prazeres
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Duarte Miguel F Prazeres
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