Expression and purification of the Saccharomyces cerevisiae alpha-factor receptor (Ste2p), a 7-transmembrane-segment G protein-coupled receptor.
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Identification of novel ErbB3-interacting factors using the split-ubiquitin membrane yeast two-hybrid systemHeterotrimeric G Protein-coupled Receptor Signaling in Yeast Mating Pheromone ResponseSubstrate preference is altered by mutations in the fifth transmembrane domain of Ptr2p, the di/tri-peptide transporter of Saccharomyces cerevisiae.Specific α-arrestins negatively regulate Saccharomyces cerevisiae pheromone response by down-modulating the G-protein-coupled receptor Ste2.ABC transporter Pdr10 regulates the membrane microenvironment of Pdr12 in Saccharomyces cerevisiae.Invited review: GPCR structural characterization: Using fragments as building blocks to determine a complete structure.Biosynthesis and biophysical analysis of domains of a yeast G protein-coupled receptor.Ultrasensitive magnetic biosensor for homogeneous immunoassay.Residues in the first extracellular loop of a G protein-coupled receptor play a role in signal transduction.Analysis of ligand-receptor cross-linked fragments by mass spectrometry.Affinity purification and characterization of a G-protein coupled receptor, Saccharomyces cerevisiae Ste2p.Cross-linking of a DOPA-containing peptide ligand into its G protein-coupled receptorIdentification of residue-to-residue contact between a peptide ligand and its G protein-coupled receptor using periodate-mediated dihydroxyphenylalanine cross-linking and mass spectrometrySynthesis of a-factor peptide from Saccharomyces cerevisiae and photoactive analogues via Fmoc solid phase methodologyHomo-oligomeric complexes of the yeast alpha-factor pheromone receptor are functional units of endocytosis.Changes in conformation at the cytoplasmic ends of the fifth and sixth transmembrane helices of a yeast G protein-coupled receptor in response to ligand binding.Identification of destabilizing and stabilizing mutations of Ste2p, a G protein-coupled receptor in Saccharomyces cerevisiae.Multiple regulatory roles of the carboxy terminus of Ste2p a yeast GPCR.High-level expression in Saccharomyces cerevisiae enables isolation and spectroscopic characterization of functional human adenosine A2a receptorTesting the limits of gradient sensing.Cytoplasmic tail phosphorylation of the alpha-factor receptor is required for its ubiquitination and internalizationStructural genomics and drug discovery.Identification of specific transmembrane residues and ligand-induced interface changes involved in homo-dimer formation of a yeast G protein-coupled receptor.Yeast GPCR signaling reflects the fraction of occupied receptors, not the numberRelieving the first bottleneck in the drug discovery pipeline: using array technologies to rationalize membrane protein production.The N-terminus of the yeast G protein-coupled receptor Ste2p plays critical roles in surface expression, signaling, and negative regulation.Cell penetrating peptides and cationic antibacterial peptides: two sides of the same coin.Purification and biochemical characterization of the D6 chemokine receptorCeramide signals for initiation of yeast mating-specific cell cycle arrest.Purification of proteins using polyhistidine affinity tags.Study of the binding environment of alpha-factor in its G protein-coupled receptor using fluorescence spectroscopy.A novel yeast expression system for the overproduction of quality-controlled membrane proteins.Dynamic roles for the N-terminus of the yeast G protein-coupled receptor Ste2p.The first extracellular loop of the Saccharomyces cerevisiae G protein-coupled receptor Ste2p undergoes a conformational change upon ligand binding.Structure-odour relationships reviewed in the postgenomic era
P2860
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P2860
Expression and purification of the Saccharomyces cerevisiae alpha-factor receptor (Ste2p), a 7-transmembrane-segment G protein-coupled receptor.
description
1997 nî lūn-bûn
@nan
1997年の論文
@ja
1997年学术文章
@wuu
1997年学术文章
@zh
1997年学术文章
@zh-cn
1997年学术文章
@zh-hans
1997年学术文章
@zh-my
1997年学术文章
@zh-sg
1997年學術文章
@yue
1997年學術文章
@zh-hant
name
Expression and purification of ...... nt G protein-coupled receptor.
@en
Expression and purification of ...... nt G protein-coupled receptor.
@nl
type
label
Expression and purification of ...... nt G protein-coupled receptor.
@en
Expression and purification of ...... nt G protein-coupled receptor.
@nl
prefLabel
Expression and purification of ...... nt G protein-coupled receptor.
@en
Expression and purification of ...... nt G protein-coupled receptor.
@nl
P2093
P2860
P921
P356
P1476
Expression and purification of ...... nt G protein-coupled receptor.
@en
P2093
P2860
P304
15553-15561
P356
10.1074/JBC.272.24.15553
P407
P577
1997-06-01T00:00:00Z