Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity.
about
The phosphopantetheinyl transferases: catalysis of a post-translational modification crucial for lifeTrapping of intermediates with substrate analog HBOCoA in the polymerizations catalyzed by class III polyhydroxybutyrate (PHB) synthase from Allochromatium vinosumRalstonia eutropha H16 encodes two and possibly three intracellular Poly[D-(-)-3-hydroxybutyrate] depolymerase genesSequence analysis and structure prediction of type II Pseudomonas sp. USM 4-55 PHA synthase and an insight into its catalytic mechanism.Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic.Genetic analysis of Comamonas acidovorans polyhydroxyalkanoate synthase and factors affecting the incorporation of 4-hydroxybutyrate monomer.Structure of polyhydroxyalkanoate (PHA) synthase PhaC from Chromobacterium sp. USM2, producing biodegradable plasticsAccumulation of the PhaP phasin of Ralstonia eutropha is dependent on production of polyhydroxybutyrate in cells.Mechanistic insight with HBCH2CoA as a probe to polyhydroxybutyrate (PHB) synthasesGrowth and localization of polyhydroxybutyrate granules in Ralstonia eutropha.Enzyme-catalyzed synthesis of poly[(R)-(-)-3-hydroxybutyrate]: formation of macroscopic granules in vitro.Enhanced accumulation and changed monomer composition in polyhydroxyalkanoate (PHA) copolyester by in vitro evolution of Aeromonas caviae PHA synthase.Cloning, overproduction, and characterization of the Escherichia coli holo-acyl carrier protein synthase.Wide distribution among halophilic archaea of a novel polyhydroxyalkanoate synthase subtype with homology to bacterial type III synthasesYfcX enables medium-chain-length poly(3-hydroxyalkanoate) formation from fatty acids in recombinant Escherichia coli fadB strainsReplacement of the catalytic nucleophile cysteine-296 by serine in class II polyhydroxyalkanoate synthase from Pseudomonas aeruginosa-mediated synthesis of a new polyester: identification of catalytic residues.Structure of the Catalytic Domain of the Class I Polyhydroxybutyrate Synthase from Cupriavidus necator.Evidence for lytic transglycosylase and β-N-acetylglucosaminidase activities located at the polyhydroxyalkanoates (PHAs) granules of Thermus thermophilus HB8.Characterization of the highly active polyhydroxyalkanoate synthase of Chromobacterium sp. strain USM2.Production of a polyhydroxyalkanoate biopolymer in insect cells with a modified eucaryotic fatty acid synthase.Polyester synthases: natural catalysts for plastics.Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus.Control of poly-beta-hydroxybutyrate synthase mediated by acetyl phosphate in cyanobacteria.Mutations derived from the thermophilic polyhydroxyalkanoate synthase PhaC enhance the thermostability and activity of PhaC from Cupriavidus necator H16.Molecular characterization of the phaECHm genes, required for biosynthesis of poly(3-hydroxybutyrate) in the extremely halophilic archaeon Haloarcula marismortui.Revelation of the ability of Burkholderia sp. USM (JCM 15050) PHA synthase to polymerize 4-hydroxybutyrate monomerChemistry with an artificial primer of polyhydroxybutyrate synthase suggests a mechanism for chain termination.Efficient production of active polyhydroxyalkanoate synthase in Escherichia coli by coexpression of molecular chaperones.Alcoholytic cleavage of polyhydroxyalkanoate chains by class IV synthases induced by endogenous and exogenous ethanol.PHA bioplastics, biochemicals, and energy from crops.Cloning of the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of these genes for enhanced production of Poly(3-hydroxybutyrate) in Escherichia coli.Inhibitors of polyhydroxyalkanoate (PHA) synthases: synthesis, molecular docking, and implications.Analysis of 4-phosphopantetheinylation of polyhydroxybutyrate synthase from Ralstonia eutropha: generation of beta-alanine auxotrophic Tn5 mutants and cloning of the panD gene region.Cloning and molecular analysis of the Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) biosynthesis genes in Pseudomonas sp. strain 61-3The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production.Purification and properties of an intracellular 3-hydroxybutyrate-oligomer hydrolase (PhaZ2) in Ralstonia eutropha H16 and its identification as a novel intracellular poly(3-hydroxybutyrate) depolymerase.Cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of Aeromonas caviae.In vivo monitoring of PHA granule formation using GFP-labeled PHA synthases.Study of Class I and Class III Polyhydroxyalkanoate (PHA) Synthases with Substrates Containing a Modified Side ChainPurification of polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications for the initiation and elongation of polymer formation in vivo.
P2860
Q28659329-A2387E3D-AC34-4848-B6D7-8FAA1441EE7FQ28834047-5B3834FE-11B8-435F-ABA1-5B997095380EQ31147026-1140C4F5-5B93-49BA-BD2F-12E9D808CE98Q33262277-DB8BFD8D-76A8-4A97-9E9E-B1FB56B14B00Q33538944-F452F0B1-50AE-4C7F-9587-9E7BF615ECEFQ33720242-2E34402B-1A4E-45AC-BBA5-E4F460CD93DCQ33903206-6DD106FE-D2EE-41CE-A7D2-872B7C0ADD4CQ33996511-C48FA674-C299-40C6-B970-7F1567EEF79DQ34061280-658350FE-26DE-4126-9830-0C946D4D8765Q34104612-8891ED9F-488A-4AF0-93D7-D77F16D62423Q34112831-63CE307E-4309-4E9F-893B-F9FA49F83D72Q34114327-6275919E-E686-4E2A-913B-AFA153C5709DQ34295536-2175E748-1EEF-43B0-A0FF-D5EF2CE33168Q34334715-FD271F68-EA7E-4B33-86BC-83DA4DD44782Q34436474-0CEEFD22-6A70-4365-8696-92A369201E57Q34536317-4E4062D9-13B8-472B-8C56-454C6F2D9B52Q34542669-692ED9B4-8A9D-4988-AA15-A330E6BD80B0Q34728025-F11256B3-1209-4411-80E3-B66D491F30D7Q35077227-A08D5058-F04A-4F7D-A7E3-6EABDE59AE53Q35192125-76D812BA-2F30-4739-A40B-7E650DF677ADQ35211113-01FE9CBD-741F-43A6-8C34-ECC6B0AF280BQ35584429-AE5C0D9E-72BA-463A-91E3-3D56BDD7C1EAQ35627698-283E5398-1989-4B64-9D3E-D83679929BABQ35943096-B74B504A-2BB5-401E-A2D0-DA105317E0F2Q36137114-A82F6FD8-1A64-4237-8105-3D1DB122D1DDQ36209430-2019193A-CC06-4554-8106-EF4198F51F25Q36384278-6ACC9824-02DC-463D-89C0-6766D3089BC4Q36668238-AE07A0E5-A3E2-4C4F-9591-722FE296518FQ37545397-38E15B35-0BD5-49DC-B0DF-6B7267ED90E2Q38072150-132C4EDE-4E8B-4A84-A7D4-51C1A8F09CC4Q38548686-7227C3B6-1371-4F97-9361-DF922C92D55FQ39460137-1A615AA9-B1D2-4385-BDA3-B30A948ED5CDQ39494428-020BC53F-D29D-4944-B043-77BA15AFE91AQ39568938-3E8ED3D2-30AF-4675-ABB4-3723C14E5B9FQ39677989-DAE21F80-E668-4634-9AAE-B6C3D01A3927Q39756968-DC44CDBF-75BF-4625-A748-8A603563C4B2Q39846657-9243D417-9B7E-4D78-8837-7C1EC52D574CQ40413184-4A128589-8C4B-4407-9C44-7575758B24D5Q41310237-59612F0A-8771-4F40-84BF-2335FDAAB10AQ41848264-1282B82F-1875-49EA-924F-F4A96502A69B
P2860
Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity.
description
1994 nî lūn-bûn
@nan
1994年の論文
@ja
1994年学术文章
@wuu
1994年学术文章
@zh-cn
1994年学术文章
@zh-hans
1994年学术文章
@zh-my
1994年学术文章
@zh-sg
1994年學術文章
@yue
1994年學術文章
@zh
1994年學術文章
@zh-hant
name
Overexpression and purificatio ...... cation for catalytic activity.
@en
Overexpression and purificatio ...... cation for catalytic activity.
@nl
type
label
Overexpression and purificatio ...... cation for catalytic activity.
@en
Overexpression and purificatio ...... cation for catalytic activity.
@nl
prefLabel
Overexpression and purificatio ...... cation for catalytic activity.
@en
Overexpression and purificatio ...... cation for catalytic activity.
@nl
P2093
P356
P1433
P1476
Overexpression and purificatio ...... cation for catalytic activity.
@en
P2093
Gerngross TU
Masamune S
Peoples OP
Sinskey AJ
P304
P356
10.1021/BI00197A035
P407
P577
1994-08-01T00:00:00Z