Tn10/IS10 transposase purification, activation, and in vitro reaction.
about
Base flipping in tn10 transposition: an active flip and capture mechanismCyclic changes in the affinity of protein-DNA interactions drive the progression and regulate the outcome of the Tn10 transposition reactionTransposon-mediated generation of targeting vectors for the production of gene knockoutsDeterminants for hairpin formation in Tn10 transposition.DNA transposon-based gene vehicles - scenes from an evolutionary driveThe autoregulation of a eukaryotic DNA transposonInsertion sequencesSoluble expression, purification and characterization of the full length IS2 Transposase.Structural domains of IS10 transposase and reconstitution of transposition activity from proteolytic fragments lacking an interdomain linker.Two classes of Tn10 transposase mutants that suppress mutations in the Tn10 terminal inverted repeat.Target joining of duplicated insertion sequence IS21 is assisted by IstB protein in vitroThe global regulator H-NS acts directly on the transpososome to promote Tn10 transpositionThe two single-strand cleavages at each end of Tn10 occur in a specific order during transposition.Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage muDevelopment of an in vitro integration assay for the Bacteroides conjugative transposon CTnDOT.A direct transposon insertion tool for modification and functional analysis of viral genomes.A simple topological filter in a eukaryotic transposon as a mechanism to suppress genome instability.Base flipping in V(D)J recombination: insights into the mechanism of hairpin formation, the 12/23 rule, and the coordination of double-strand breaksControl of transposase activity within a transpososome by the configuration of the flanking DNA segment of the transposon.Transposon for protein engineeringTn552 transposase catalyzes concerted strand transfer in vitro.Identification and characterization of a pre-cleavage synaptic complex that is an early intermediate in Tn10 transpositionTn 10 transposition in vivo: temporal separation of cleavages at the two transposon ends and roles of terminal basepairs subsequent to interaction of endsProtein-DNA contacts and conformational changes in the Tn10 transpososome during assembly and activation for cleavage.Early intermediates of mariner transposition: catalysis without synapsis of the transposon ends suggests a novel architecture of the synaptic complexThe positive and negative regulation of Tn10 transposition by IHF is mediated by structurally asymmetric transposon arms.Isolation and characterization of Tn7 transposase gain-of-function mutants: a model for transposase activation.An assay to monitor the activity of DNA transposition complexes yields a general quality control measure for transpositional recombination reactions.Tn552 transposase purification and in vitro activities.Multiple roles for divalent metal ions in DNA transposition: distinct stages of Tn10 transposition have different Mg2+ requirements.IS10/Tn10 transposition efficiently accommodates diverse transposon end configurations.The Tn7 transposase is a heteromeric complex in which DNA breakage and joining activities are distributed between different gene products.Expanding the Limits of Thermoacidophily in the Archaeon Sulfolobus solfataricus by Adaptive Evolution.Factors responsible for target site selection in Tn10 transposition: a role for the DDE motif in target DNA capture.Factors affecting transposition of the Himar1 mariner transposon in vitroTransposition of Mboumar-9: identification of a new naturally active mariner-family transposonH-NS binds with high affinity to the Tn10 transpososome and promotes transpososome stabilization.H-NS mediates the dissociation of a refractory protein-DNA complex during Tn10/IS10 transposition.Transposition of the human Hsmar1 transposon: rate-limiting steps and the importance of the flanking TA dinucleotide in second strand cleavage.Transposase subunit architecture and its relationship to genome size and the rate of transposition in prokaryotes and eukaryotes
P2860
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P2860
Tn10/IS10 transposase purification, activation, and in vitro reaction.
description
1994 nî lūn-bûn
@nan
1994年の論文
@ja
1994年学术文章
@wuu
1994年学术文章
@zh-cn
1994年学术文章
@zh-hans
1994年学术文章
@zh-my
1994年学术文章
@zh-sg
1994年學術文章
@yue
1994年學術文章
@zh
1994年學術文章
@zh-hant
name
Tn10/IS10 transposase purification, activation, and in vitro reaction.
@en
Tn10/IS10 transposase purification, activation, and in vitro reaction.
@nl
type
label
Tn10/IS10 transposase purification, activation, and in vitro reaction.
@en
Tn10/IS10 transposase purification, activation, and in vitro reaction.
@nl
prefLabel
Tn10/IS10 transposase purification, activation, and in vitro reaction.
@en
Tn10/IS10 transposase purification, activation, and in vitro reaction.
@nl
P1476
Tn10/IS10 transposase purification, activation, and in vitro reaction.
@en
P2093
Chalmers RM
Kleckner N
P304
P407
P577
1994-03-01T00:00:00Z