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X-Ray Structure and Site-Directed Mutagenesis Analysis of the Escherichia coli Colicin M Immunity ProteinFunctional and Structural Characterization of PaeM, a Colicin M-like Bacteriocin Produced by Pseudomonas aeruginosaPreparative scale cell-free production and quality optimization of MraY homologues in different expression modesPurification and characterization of the bacterial UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase WecAPreparative enzymatic synthesis of polyprenyl-pyrophosphoryl-N-acetylglucosamine, an essential lipid intermediate for the biosynthesis of various bacterial cell envelope polymersMembrane Topology and Biochemical Characterization of the Escherichia coli BacA Undecaprenyl-Pyrophosphate PhosphatasePurification and characterization of the bacterial MraY translocase catalyzing the first membrane step of peptidoglycan biosynthesis.The bacA gene of Escherichia coli encodes an undecaprenyl pyrophosphate phosphatase activity.Human- and plant-pathogenic Pseudomonas species produce bacteriocins exhibiting colicin M-like hydrolase activity towards peptidoglycan precursorsDeciphering the metabolism of undecaprenyl-phosphate: the bacterial cell-wall unit carrier at the membrane frontierSynthesis and biological evaluation of a diazepanone-based library of liposidomycins analogs as MraY inhibitors.Identification of FtsW as a transporter of lipid-linked cell wall precursors across the membraneThe MurE synthetase from Thermotoga maritima is endowed with an unusual D-lysine adding activity.Fluorescence detection-based functional assay for high-throughput screening for MraY.The biosynthesis of peptidoglycan lipid-linked intermediates.Pectocin M1 (PcaM1) Inhibits Escherichia coli Cell Growth and Peptidoglycan Biosynthesis through Periplasmic ExpressionCharacterization of colicin M and its orthologs targeting bacterial cell wall peptidoglycan biosynthesis.Deciphering the catalytic domain of colicin M, a peptidoglycan lipid II-degrading enzyme.Importance of the conserved residues in the peptidoglycan glycosyltransferase module of the class A penicillin-binding protein 1b of Escherichia coli.Structure-based site-directed mutagenesis of the UDP-MurNAc-pentapeptide-binding cavity of the FemX alanyl transferase from Weissella viridescensColicin M, a peptidoglycan lipid-II-degrading enzyme: potential use for antibacterial means?Colicin M exerts its bacteriolytic effect via enzymatic degradation of undecaprenyl phosphate-linked peptidoglycan precursors.Aminoacyl-tRNA recognition by the FemXWv transferase for bacterial cell wall synthesis.Toward analogues of MraY natural inhibitors: synthesis of 5'-triazole-substituted-aminoribosyl uridines through a Cu-catalyzed azide-alkyne cycloaddition.Colicin M hydrolyses branched lipids II from Gram-positive bacteria.
P50
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P50
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Ahmed Bouhss
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Ahmed Bouhss
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Ahmed Bouhss
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Ahmed Bouhss
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Ahmed Bouhss
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Ahmed Bouhss
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Ahmed Bouhss
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Ahmed Bouhss
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Ahmed Bouhss
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Ahmed Bouhss
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