Fine affinity discrimination by yeast surface display and flow cytometry
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Applications of Yeast Surface Display for Protein EngineeringMolecular evolution of antibody cross-reactivity for two subtypes of type A botulinum neurotoxinYeast mating for combinatorial Fab library generation and surface displayEngineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular AccumulationToward low-cost affinity reagents: lyophilized yeast-scFv probes specific for pathogen antigens.Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library.Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity.Rapid isolation of high-affinity protein binding peptides using bacterial display.Affinity maturation of Fab antibody fragments by fluorescent-activated cell sorting of yeast-displayed libraries.Efficient library construction by in vivo recombination with a telomere-originated autonomously replicating sequence of Hansenula polymorphaRecombinant antibodies: a novel approach to cancer diagnosis and therapy.Isolation of anti-CD22 Fv with high affinity by Fv display on human cells.Optimizing the affinity and specificity of proteins with molecular display.Isolating and engineering human antibodies using yeast surface display.A decade of yeast surface display technology: where are we now?Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptidesPicomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis, and loop shuffling.Lentivirus display: stable expression of human antibodies on the surface of human cells and virus particlesA yeast display immunoprecipitation method for efficient isolation and characterization of antigens.Highly avid magnetic bead capture: an efficient selection method for de novo protein engineering utilizing yeast surface display.Antibody library screens using detergent-solubilized mammalian cell lysates as antigen sources.Comprehensive analysis of yeast surface displayed cDNA library selection outputs by exon microarray to identify novel protein-ligand interactions.Combining phage and staphylococcal surface display for generation of ErbB3-specific Affibody molecules.Synergistic capture of Clostridium botulinum type A neurotoxin by scFv antibodies to novel epitopes.Bacterial display in combinatorial protein engineering.Phage display and pharmacogenomics.Affinity maturation of anti-TNF-alpha scFv with somatic hypermutation in non-B cells.Surface display of a single-domain antibody library on Gram-positive bacteria.Antibody affinity maturation using yeast display with detergent-solubilized membrane proteins as antigen sourcesIn vitro affinity screening of protein and peptide binders by megavalent bead surface display.A mammalian cell based FACS-panning platform for the selection of HIV-1 envelopes for vaccine developmentThe Shark Strikes Twice: Hypervariable Loop 2 of Shark IgNAR Antibody Variable Domains and Its Potential to Function as an Autonomous Paratope.Combining Phage and Yeast Cell Surface Antibody Display to Identify Novel Cell Type-Selective Internalizing Human Monoclonal AntibodiesIsolation of a pH-Sensitive IgNAR Variable Domain from a Yeast-Displayed, Histidine-Doped Master Library.Investigating affinity-maturation strategies and reproducibility of fluorescence-activated cell sorting using a recombinant ADAPT library displayed on staphylococci.A platform for constructing, evaluating, and screening bioconjugates on the yeast surface.A Shorter Route to Antibody Binders via Quantitative in vitro Bead-Display Screening and Consensus Analysis.CellSort: a support vector machine tool for optimizing fluorescence-activated cell sorting and reducing experimental effort.Characterization and directed evolution of a methyl-binding domain protein for high-sensitivity DNA methylation analysisTitratable Avidity Reduction Enhances Affinity Discrimination in Mammalian Cellular Selections of Yeast-Displayed Ligands.
P2860
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P2860
Fine affinity discrimination by yeast surface display and flow cytometry
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wetenschappelijk artikel
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wissenschaftlicher Artikel
@de
name
Fine affinity discrimination by yeast surface display and flow cytometry
@en
Fine affinity discrimination by yeast surface display and flow cytometry
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type
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Fine affinity discrimination by yeast surface display and flow cytometry
@en
Fine affinity discrimination by yeast surface display and flow cytometry
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prefLabel
Fine affinity discrimination by yeast surface display and flow cytometry
@en
Fine affinity discrimination by yeast surface display and flow cytometry
@nl
P356
P1476
Fine affinity discrimination by yeast surface display and flow cytometry
@en
P2093
J J VanAntwerp
K D Wittrup
P356
10.1021/BP990133S