A Genetically Encoded Norbornene Amino Acid for the Mild and Selective Modification of Proteins in a Copper-Free Click Reaction
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Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion toolStructural basis for the site-specific incorporation of lysine derivatives into proteinsStructure and reaction mechanism of pyrrolysine synthase (PylD)Structural insights into incorporation of norbornene amino acids for click modification of proteinsThe formation of pyrroline and tetrahydropyridine rings in amino acids catalyzed by pyrrolysine synthase (PylD)A Minimal, Unstrained S-Allyl Handle for Pre-Targeting Diels-Alder Bioorthogonal Labeling in Live CellsPhoto-Triggered Click Chemistry for Biological ApplicationsBioconjugation of therapeutic proteins and enzymes using the expanded set of genetically encoded amino acids.Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double-labelling and FRETTetrazine ligation for chemical proteomics.Photoclick chemistry: a fluorogenic light-triggered in vivo ligation reaction.Two rapid catalyst-free click reactions for in vivo protein labeling of genetically encoded strained alkene/alkyne functionalities.Biocompatible click chemistry enabled compartment-specific pH measurement inside E. coli.A Bioorthogonal Chemical Reporter of Viral Infection.Protein conjugation with genetically encoded unnatural amino acids.A genetically encoded alkyne directs palladium-mediated protein labeling on live mammalian cell surfaceGenetically encoded cyclopropene directs rapid, photoclick-chemistry-mediated protein labeling in mammalian cells.Imaging beyond the proteomeGenetic Encoding of bicyclononynes and trans-cyclooctenes for site-specific protein labeling in vitro and in live mammalian cells via rapid fluorogenic Diels-Alder reactions.A genetically encoded acrylamide functionality.Tagging and Enriching Proteins Enables Cell-Specific Proteomics.trans-Cyclooctene--a stable, voracious dienophile for bioorthogonal labeling.SiRNA Crosslinked Nanoparticles for the Treatment of Inflammation-induced Liver Injury.Bioorthogonal labelling of biomolecules: new functional handles and ligation methods.Selective chemical protein modification.Design strategies for bioorthogonal smart probes6-[6-(Pyridin-2-yl)-1,2,4,5-tetra-zin-3-yl]pyridin-3-amine monohydrate.Genetically designed biomolecular capping system for mesoporous silica nanoparticles enables receptor-mediated cell uptake and controlled drug release.Bioorthogonal Chemistry-Introduction and Overview.Micelle-Enhanced Bioorthogonal Labeling of Genetically Encoded Azido Groups on the Lipid-Embedded Surface of a GPCR.Cycloadditions for Studying Nucleic Acids.A cascading reaction sequence involving ligand-directed azaelectrocyclization and autooxidation-induced fluorescence recovery enables visualization of target proteins on the surfaces of live cells.Mechanism-Based Fluorogenic trans-Cyclooctene-Tetrazine Cycloaddition.Designing logical codon reassignment - Expanding the chemistry in biology.Bio-orthogonal Fluorescent Labelling of Biopolymers through Inverse-Electron-Demand Diels-Alder ReactionsExpanding the genetic code for photoclick chemistry in E. coli, mammalian cells, and A. thaliana.Live-cell imaging of cyclopropene tags with fluorogenic tetrazine cycloadditions.Genetic encoding of a bicyclo[6.1.0]nonyne-charged amino acid enables fast cellular protein imaging by metal-free ligation.Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy.Two-step protein labeling by using lipoic acid ligase with norbornene substrates and subsequent inverse-electron demand Diels-Alder reaction.
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P2860
A Genetically Encoded Norbornene Amino Acid for the Mild and Selective Modification of Proteins in a Copper-Free Click Reaction
description
scientific article published on 21 March 2012
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wetenschappelijk artikel
@nl
наукова стаття, опублікована в березні 2012
@uk
name
A Genetically Encoded Norborne ...... n a Copper-Free Click Reaction
@en
A Genetically Encoded Norborne ...... n a Copper-Free Click Reaction
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type
label
A Genetically Encoded Norborne ...... n a Copper-Free Click Reaction
@en
A Genetically Encoded Norborne ...... n a Copper-Free Click Reaction
@nl
prefLabel
A Genetically Encoded Norborne ...... n a Copper-Free Click Reaction
@en
A Genetically Encoded Norborne ...... n a Copper-Free Click Reaction
@nl
P2093
P2860
P356
P1476
A genetically encoded norborne ...... n a copper-free click reaction
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P2093
Christian Deiml
Emine Kaya
Stefan Prill
Viviana S Fluxa
P2860
P304
P356
10.1002/ANIE.201109252
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P577
2012-03-21T00:00:00Z