about
One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genomeEssential genes of a minimal bacterium.Cell-free cloning using phi29 DNA polymerase.Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotidesSimilarity-based gene detection: using COGs to find evolutionarily-conserved ORFsEnzymatic assembly of DNA molecules up to several hundred kilobasesComplete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genomeCreation of a bacterial cell controlled by a chemically synthesized genomeMapping phosphoproteins in Mycoplasma genitalium and Mycoplasma pneumoniaeDirect transfer of whole genomes from bacteria to yeast.Cloning whole bacterial genomes in yeast.Transcriptional analysis of the conserved ftsZ gene cluster in Mycoplasma genitalium and Mycoplasma pneumoniae.Designer diatom episomes delivered by bacterial conjugation.Genome transplantation in bacteria: changing one species to another.DNA sequencing: bench to bedside and beyond.Bacterial genome reduction using the progressive clustering of deletions via yeast sexual cycling.Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast.Minimal Cells-Real and Imagined.Assembly of eukaryotic algal chromosomes in yeast.Rescue of mutant fitness defects using in vitro reconstituted designer transposons in Mycoplasma mycoides.A systems biology tour de force for a near-minimal bacterium.Editorial-synthetic biology.Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing.Cloning the Acholeplasma laidlawii PG-8A genome in Saccharomyces cerevisiae as a yeast centromeric plasmid.Chemical synthesis of the mouse mitochondrial genome.
P50
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P50
name
Clyde A Hutchison
@en
Clyde A Hutchison
@nl
type
label
Clyde A Hutchison
@en
Clyde A Hutchison
@nl
prefLabel
Clyde A Hutchison
@en
Clyde A Hutchison
@nl