Two-dimensional gel electrophoretic method for mapping DNA replicons.
about
Choosing a suitable method for the identification of replication origins in microbial genomesInitiation sites are distributed at frequent intervals in the Chinese hamster dihydrofolate reductase origin of replication but are used with very different efficienciesThe dihydrofolate reductase origin of replication does not contain any nonredundant genetic elements required for origin activity.Initiation of replication in the Chinese hamster dihydrofolate reductase domain.Mammalian origins of replication.The plant amino acid mimosine may inhibit initiation at origins of replication in Chinese hamster cells.Initiation of DNA replication in the dihydrofolate reductase locus is confined to the early S period in CHO cells synchronized with the plant amino acid mimosine.A winding road to origin discoverySpecific signals at the 3' end of the DHFR gene define one boundary of the downstream origin of replication.A revisionist replicon model for higher eukaryotic genomes.Replication initiation sites are distributed widely in the amplified CHO dihydrofolate reductase domain.Mapping of replication initiation sites in mammalian genomes by two-dimensional gel analysis: stabilization and enrichment of replication intermediates by isolation on the nuclear matrix.Replication forks are associated with the nuclear matrixCharacterizing replication intermediates in the amplified CHO dihydrofolate reductase domain by two novel gel electrophoretic techniques.Composite patterns in neutral/neutral two-dimensional gels demonstrate inefficient replication origin usage.The Chinese hamster dihydrofolate reductase origin consists of multiple potential nascent-strand start sitesLagging-strand, early-labelling, and two-dimensional gel assays suggest multiple potential initiation sites in the Chinese hamster dihydrofolate reductase originSequence and context effects on origin function in mammalian cells.Replication of bovine papillomavirus type 1 (BPV-1) DNA in Saccharomyces cerevisiae following infection with BPV-1 virions.Inhibition of initiation of simian virus 40 DNA replication during acute response of cells irradiated by ultraviolet light.Replication initiation and elongation fork rates within a differentially expressed human multicopy locus in early S phaseDetermination of DNA replication kinetics in synchronized human cells using a PCR-based assay.The hunt for origins of DNA replication in multicellular eukaryotesThe Human RNA Polymerase I Transcription Terminator Complex Acts as a Replication Fork Barrier That Coordinates the Progress of Replication with rRNA Transcription Activity.Replicative intermediates of human papillomavirus type 11 in laryngeal papillomas: site of replication initiation and direction of replication.Silencers, silencing, and heritable transcriptional statesEvidence for a switch in the mode of human papillomavirus type 16 DNA replication during the viral life cycleAmbiguities in results obtained with 2D gel replicon mapping techniquesClose association of a DNA replication origin and an ARS element on chromosome III of the yeast, Saccharomyces cerevisiaeMultiple replication origins are used during Drosophila chorion gene amplificationImmunoglobulin heavy chain enhancer is located near or in an initiation zone of chromosomal DNA replication.Identification of an origin of bidirectional DNA replication in the ubiquitously expressed mammalian CAD geneRegulation of the replication of the murine immunoglobulin heavy chain gene locus: evaluation of the role of the 3' regulatory region.Two alleles of a developmentally regulated alpha-tubulin locus in Physarum polycephalum replicate on different schedulesLocalization of a DNA replication origin and termination zone on chromosome III of Saccharomyces cerevisiae.Evidence that replication initiates at only some of the potential origins in each oligomeric form of bovine papillomavirus type 1 DNAAn initiation zone of chromosomal DNA replication located upstream of the c-myc gene in proliferating HeLa cellsIsolation of human sequences that replicate autonomously in human cellsInitiation and termination of DNA replication in human rRNA genes.Organization of replication of ribosomal DNA in Saccharomyces cerevisiae.
P2860
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P2860
Two-dimensional gel electrophoretic method for mapping DNA replicons.
description
1988 nî lūn-bûn
@nan
1988年の論文
@ja
1988年論文
@yue
1988年論文
@zh-hant
1988年論文
@zh-hk
1988年論文
@zh-mo
1988年論文
@zh-tw
1988年论文
@wuu
1988年论文
@zh
1988年论文
@zh-cn
name
Two-dimensional gel electrophoretic method for mapping DNA replicons.
@en
type
label
Two-dimensional gel electrophoretic method for mapping DNA replicons.
@en
prefLabel
Two-dimensional gel electrophoretic method for mapping DNA replicons.
@en
P2860
P356
P1476
Two-dimensional gel electrophoretic method for mapping DNA replicons.
@en
P2093
J A Huberman
K A Nawotka
P2860
P304
P356
10.1128/MCB.8.4.1408
P407
P577
1988-04-01T00:00:00Z