Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering.
about
Structure, function, and evolution of bacterial ATP-binding cassette systemsA genetically encoded, high-signal-to-noise maltose sensorDynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors.Quantitative two-photon imaging of fluorescent biosensorsIn situ monitoring of intracellular glucose and glutamine in CHO cell cultureResonance Energy Transfer Between Luminescent Quantum Dots and Diverse Fluorescent Protein AcceptorsProtonophore- and pH-insensitive glucose and sucrose accumulation detected by FRET nanosensors in Arabidopsis root tipsNanosensor detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle.Engineering and rapid selection of a low-affinity glucose/galactose-binding protein for a glucose biosensorGrowth-limiting intracellular metabolites in yeast growing under diverse nutrient limitations.Imaging approach for monitoring cellular metabolites and ions using genetically encoded biosensorsAnalyzing cell physiology in C. elegans with fluorescent ratiometric reporters.Optical sensors for measuring dynamic changes of cytosolic metabolite levels in yeast.A bacteria colony-based screen for optimal linker combinations in genetically encoded biosensorsFacilitative plasma membrane transporters function during ER transitIn vivo biochemistry: quantifying ion and metabolite levels in individual cells or cultures of yeast.Optical sensors for monitoring dynamic changes of intracellular metabolite levels in mammalian cells.Visualization of glutamine transporter activities in living cells using genetically encoded glutamine sensorsGenetically encoded biosensors based on engineered fluorescent proteins.Abscisic acid dynamics in roots detected with genetically encoded FRET sensors.Mining the Sinorhizobium meliloti transportome to develop FRET biosensors for sugars, dicarboxylates and cyclic polyols.A genetically encoded FRET lactate sensor and its use to detect the Warburg effect in single cancer cells.High resolution measurement of the glycolytic rate.Molecular basis for the action of a dietary flavonoid revealed by the comprehensive identification of apigenin human targets.Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells.Development of a high-dynamic range, GFP-based FRET probe sensitive to oxidative microenvironments.Rapid, randomized development of genetically encoded FRET sensors for small molecules.A genetically encoded Förster resonance energy transfer sensor for monitoring in vivo trehalose-6-phosphate dynamics.Biofuel metabolic engineering with biosensors.Shining light on signaling and metabolic networks by genetically encoded biosensors.Construction of Protein Switches by Domain Insertion and Directed Evolution.Glutamate biosensor imaging reveals dysregulation of glutamatergic pathways in a model of developmental cortical malformationMultiplexed visualization of dynamic signaling networks using genetically encoded fluorescent protein-based biosensorsOptical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters.Extrasynaptic glutamate and inhibitory neurotransmission modulate ganglion cell participation during glutamatergic retinal waves.Cellular metabolomics of Escherchia coli.Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants.Quantitative imaging for discovery and assembly of the metabo-regulome.A novel analytical method for in vivo phosphate trackingGenetically encoded fluorescent sensors for studying healthy and diseased nervous systems.
P2860
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P2860
Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering.
description
2005 nî lūn-bûn
@nan
2005年の論文
@ja
2005年論文
@yue
2005年論文
@zh-hant
2005年論文
@zh-hk
2005年論文
@zh-mo
2005年論文
@zh-tw
2005年论文
@wuu
2005年论文
@zh
2005年论文
@zh-cn
name
Construction and optimization ...... irational protein engineering.
@en
Construction and optimization ...... irational protein engineering.
@nl
type
label
Construction and optimization ...... irational protein engineering.
@en
Construction and optimization ...... irational protein engineering.
@nl
prefLabel
Construction and optimization ...... irational protein engineering.
@en
Construction and optimization ...... irational protein engineering.
@nl
P2093
P2860
P356
P1433
P1476
Construction and optimization ...... mirational protein engineering
@en
P2093
Karen Deuschle
Leonid Kozhukh
Loren L Looger
Marcus Fehr
Sakiko Okumoto
P2860
P304
P356
10.1110/PS.051508105
P577
2005-09-01T00:00:00Z