Guildfield Missionary Baptist Church is a historic African-American church on Guildfield Church Road in South Guthrie, Tennessee. The congregation was started in 1868 with meetings at a home in South Guthrie. It was formally organized in 1869 and affiliated with the Consolidated American Missionary Baptist Convention, a forerunner to the National Baptist Convention. Land for a church was acquired in 1871. The congregation completed a new frame church building before 1882. It was replaced at the same location by a second frame building in the 1880s and a third frame church in the 1890s.