Joseph H. Prefontaine (1859 – December 26, 1937) was a farmer and political figure in Manitoba. He represented Assiniboia from 1903 to 1907 in the Legislative Assembly of Manitoba as a Liberal. He came to Manitoba in 1830, settling near . He was married to Georgina Senecal. Prefontaine was reeve for the Rural Municipality of St. Francois Xavier from 1901 to 1903. He was defeated when he ran for reelection to the Manitoba assembly in 1907. Prefontaine later served on the council for the Rural Municipality of Cartier. His brother Albert Prefontaine also served in the provincial assembly.