An amplified chromosomal sequence that includes the gene for dihydrofolate reductase initiates replication within specific restriction fragments
about
The HeLa Pur factor binds single-stranded DNA at a specific element conserved in gene flanking regions and origins of DNA replicationCondensation by DNA looping facilitates transfer of large DNA molecules into mammalian cellsSequence of cDNA comprising the human pur gene and sequence-specific single-stranded-DNA-binding properties of the encoded proteinRIP60, a mammalian origin-binding protein, enhances DNA bending near the dihydrofolate reductase origin of replicationPurification of RIP60 and RIP100, mammalian proteins with origin-specific DNA-binding and ATP-dependent DNA helicase activitiesPeaks cloaked in the mist: the landscape of mammalian replication originsA potential role for mini-chromosome maintenance (MCM) proteins in initiation at the dihydrofolate reductase replication origin.The plant amino acid mimosine may inhibit initiation at origins of replication in Chinese hamster cells.The promoter of the Chinese hamster ovary dihydrofolate reductase gene regulates the activity of the local origin and helps define its boundariesBubble-chip analysis of human origin distributions demonstrates on a genomic scale significant clustering into zones and significant association with transcription.Initiation of DNA replication in the dihydrofolate reductase locus is confined to the early S period in CHO cells synchronized with the plant amino acid mimosine.A winding road to origin discoverySpecific signals at the 3' end of the DHFR gene define one boundary of the downstream origin of replication.Activation of a mammalian origin of replication by chromosomal rearrangement.Replication initiation sites are distributed widely in the amplified CHO dihydrofolate reductase domain.Similar 150-kilobase DNA sequences are amplified in independently derived methotrexate-resistant Chinese hamster cells.Mapping of replication initiation sites in mammalian genomes by two-dimensional gel analysis: stabilization and enrichment of replication intermediates by isolation on the nuclear matrix.Multiple origins of replication in the dihydrofolate reductase amplicons of a methotrexate-resistant chinese hamster cell line.Identification and characterization of a gene that is coamplified with dihydrofolate reductase in a methotrexate-resistant CHO cell lineReplication in the amplified dihydrofolate reductase domain in CHO cells may initiate at two distinct sites, one of which is a repetitive sequence element.High-resolution mapping of replication fork movement through the amplified dihydrofolate reductase domain in CHO cells by in-gel renaturation analysis.Matrix attachment regions are positioned near replication initiation sites, genes, and an interamplicon junction in the amplified dihydrofolate reductase domain of Chinese hamster ovary cells.The dihydrofolate reductase amplicons in different methotrexate-resistant Chinese hamster cell lines share at least a 273-kilobase core sequence, but the amplicons in some cell lines are much larger and are remarkably uniform in structure.Mimosine, a novel inhibitor of DNA replication, binds to a 50 kDa protein in Chinese hamster cellsA general protocol for evaluating the specific effects of DNA replication inhibitors.Characterizing replication intermediates in the amplified CHO dihydrofolate reductase domain by two novel gel electrophoretic techniques.Composite patterns in neutral/neutral two-dimensional gels demonstrate inefficient replication origin usage.The Chinese hamster dihydrofolate reductase origin consists of multiple potential nascent-strand start sitesLagging-strand, early-labelling, and two-dimensional gel assays suggest multiple potential initiation sites in the Chinese hamster dihydrofolate reductase originInitiation of DNA replication in eukaryotes: questioning the origin.Identification of primary initiation sites for DNA replication in the hamster dihydrofolate reductase gene initiation zone.Dynamics of association of origins of DNA replication with the nuclear matrix during the cell cycleIn search of the holy replicatorReplication initiation patterns in the beta-globin loci of totipotent and differentiated murine cells: evidence for multiple initiation regions.Modular sequence elements associated with origin regions in eukaryotic chromosomal DNA.The hunt for origins of DNA replication in multicellular eukaryotesGuide to identification of origins of DNA replication in eukaryotic cell chromosomes.Emetine allows identification of origins of mammalian DNA replication by imbalanced DNA synthesis, not through conservative nucleosome segregationRegulation of cell cycle progression and nuclear affinity of the retinoblastoma protein by protein phosphatases.Effect of anti-cruciform DNA monoclonal antibodies on DNA replication.
P2860
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P2860
An amplified chromosomal sequence that includes the gene for dihydrofolate reductase initiates replication within specific restriction fragments
description
1982 nî lūn-bûn
@nan
1982 թուականի Յուլիսին հրատարակուած գիտական յօդուած
@hyw
1982 թվականի հուլիսին հրատարակված գիտական հոդված
@hy
1982年の論文
@ja
1982年論文
@yue
1982年論文
@zh-hant
1982年論文
@zh-hk
1982年論文
@zh-mo
1982年論文
@zh-tw
1982年论文
@wuu
name
An amplified chromosomal seque ...... specific restriction fragments
@ast
An amplified chromosomal seque ...... specific restriction fragments
@en
type
label
An amplified chromosomal seque ...... specific restriction fragments
@ast
An amplified chromosomal seque ...... specific restriction fragments
@en
prefLabel
An amplified chromosomal seque ...... specific restriction fragments
@ast
An amplified chromosomal seque ...... specific restriction fragments
@en
P2860
P356
P1476
An amplified chromosomal seque ...... specific restriction fragments
@en
P2093
J L Hamlin
N H Heintz
P2860
P304
P356
10.1073/PNAS.79.13.4083
P407
P577
1982-07-01T00:00:00Z