Escherichia coli skp chaperone coexpression improves solubility and phage display of single-chain antibody fragments.
about
Selection of gonadotrophin surge attenuating factor phage antibodies by bioassay.Encapsulated Escherichia coli in alginate beads capable of secreting a heterologous pectin lyaseExploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage DisplayThe SurA periplasmic PPIase lacking its parvulin domains functions in vivo and has chaperone activity.Translation levels control multi-spanning membrane protein expressionStrategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli.Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS).Characterization of a key neutralizing epitope on pertussis toxin recognized by monoclonal antibody 1B7.Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries.Rapid isolation of a single-chain antibody against the cyanobacterial toxin microcystin-LR by phage display and its use in the immunoaffinity concentration of microcystins from water.Automated selection of aptamers against protein targets translated in vitro: from gene to aptamer.Passive protection against anthrax by using a high-affinity antitoxin antibody fragment lacking an Fc regionHapten mediated display and pairing of recombinant antibodies accelerates assay assembly for biothreat countermeasuresDetection of anthrax toxin in the serum of animals infected with Bacillus anthracis by using engineered immunoassaysRoles of DegP in prevention of protein misfolding in the periplasm upon overexpression of penicillin acylase in Escherichia coli.High-sensitivity monoclonal antibodies specific for homoserine lactones protect mice from lethal Pseudomonas aeruginosa infections.Therapeutic antibody engineering by high efficiency cell screening.Secretion of anti-Plasmodium effector proteins from a natural Pantoea agglomerans isolate by using PelB and HlyA secretion signalsDefining the complementarities between antibodies and haptens to refine our understanding and aid the prediction of a successful binding interaction.Importance of Hypervariable Region 2 for Stability and Affinity of a Shark Single-Domain Antibody Specific for Ebola Virus NucleoproteinUse of folding modulators to improve heterologous protein production in Escherichia coli.Engineering production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp.Tuned Escherichia coli as a host for the expression of disulfide-rich proteins.Immunoglobulin domains in Escherichia coli and other enterobacteria: from pathogenesis to applications in antibody technologies.Using protein-DNA chimeras to detect and count small numbers of molecules.Integrating scFv into xMAP Assays for the Detection of Marine ToxinsModification of the ionizing radiation response in living cells by an scFv against the DNA-dependent protein kinase.Co-expression of Skp and FkpA chaperones improves cell viability and alters the global expression of stress response genes during scFvD1.3 production.Expression of active human sialyltransferase ST6GalNAcI in Escherichia coli.Substrate oscillations boost recombinant protein release from Escherichia coli.Generation of High-Sensitivity Monoclonal Antibodies Specific for Homoserine Lactones.The periplasmic E. coli chaperone Skp is a trimer in solution: biophysical and preliminary crystallographic characterization.High-level production of a single chain antibody against anthrax toxin in Escherichia coli by high cell density cultivation.Generating FN3-Based Affinity Reagents Through Phage DisplayDisplay of aggregation-prone ligand binding domain of human PPAR gamma on surface of bacteriophage lambda
P2860
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P2860
Escherichia coli skp chaperone coexpression improves solubility and phage display of single-chain antibody fragments.
description
1999 nî lūn-bûn
@nan
1999 թուականի Ապրիլին հրատարակուած գիտական յօդուած
@hyw
1999 թվականի ապրիլին հրատարակված գիտական հոդված
@hy
1999年の論文
@ja
1999年論文
@yue
1999年論文
@zh-hant
1999年論文
@zh-hk
1999年論文
@zh-mo
1999年論文
@zh-tw
1999年论文
@wuu
name
Escherichia coli skp chaperone ...... ngle-chain antibody fragments.
@ast
Escherichia coli skp chaperone ...... ngle-chain antibody fragments.
@en
type
label
Escherichia coli skp chaperone ...... ngle-chain antibody fragments.
@ast
Escherichia coli skp chaperone ...... ngle-chain antibody fragments.
@en
prefLabel
Escherichia coli skp chaperone ...... ngle-chain antibody fragments.
@ast
Escherichia coli skp chaperone ...... ngle-chain antibody fragments.
@en
P921
P356
P1476
Escherichia coli skp chaperone ...... ingle-chain antibody fragments
@en
P2093
P304
P356
10.1006/PREP.1999.1035
P577
1999-04-01T00:00:00Z