Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries.
about
Crystal Structure of the Engineered Neutralizing Antibody M18 Complexed to Domain 4 of the Anthrax Protective AntigenDirected evolution: tailoring biocatalysts for industrial applicationsDirected evolution of a G protein-coupled receptor for expression, stability, and binding selectivityA dual-mode surface display system for the maturation and production of monoclonal antibodies in glyco-engineered Pichia pastorisMulti-constraint computational design suggests that native sequences of germline antibody H3 loops are nearly optimal for conformational flexibilitySingle-cell characterization of autotransporter-mediated Escherichia coli surface display of disulfide bond-containing proteins.Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli.Discovery of aminoacyl-tRNA synthetase activity through cell-surface display of noncanonical amino acidsOptimization of electroporation-mediated transformation: Staphylococcus carnosus as model organism.A scFv antibody mutant isolated in a genetic screen for improved export via the twin arginine transporter pathway exhibits faster folding.Design of synthetic antibody libraries.APEx 2-hybrid, a quantitative protein-protein interaction assay for antibody discovery and engineeringHigh-affinity, human antibody-like antibody fragment (single-chain variable fragment) neutralizing the lethal factor (LF) of Bacillus anthracis by inhibiting protective antigen-LF complex formation.E-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display.Engineering antibody fragments to fold in the absence of disulfide bonds.Quantitative specificity-based display library screening identifies determinants of antibody-epitope binding specificityReconstitution and engineering of apoptotic protein interactions on the bacterial cell surface.Engineering of stable bispecific antibodies targeting IL-17A and IL-23.Engineered microbial biosensors based on bacterial two-component systems as synthetic biotechnology platforms in bioremediation and biorefinery.Progress towards recombinant anti-infective antibodies.Bacterial display in combinatorial protein engineering.Immunogenomic engineering of a plug-and-(dis)play hybridoma platform.Engineering antibody fitness and function using membrane-anchored display of correctly folded proteins.Nanohole-based surface plasmon resonance instruments with improved spectral resolution quantify a broad range of antibody-ligand binding kinetics.Passive protection against anthrax by using a high-affinity antitoxin antibody fragment lacking an Fc regionAffinity maturation of antiHER2 monoclonal antibody MIL5 using an epitope-specific synthetic phage library by computational design.Surface display of a single-domain antibody library on Gram-positive bacteria.Hapten mediated display and pairing of recombinant antibodies accelerates assay assembly for biothreat countermeasuresStabilization of the single-chain fragment variable by an interdomain disulfide bond and its effect on antibody affinity.Isolation of fully synthetic promoters for high-level gene expression in Corynebacterium glutamicum.Detection of anthrax toxin in the serum of animals infected with Bacillus anthracis by using engineered immunoassaysSelection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies.Hishot display--a new combinatorial display for obtaining target-recognizing peptides.scFv antibodies against infectious bursal disease virus isolated from a combinatorial antibody library by flow cytometry.Development trends for generation of single-chain antibody fragments.Rapid isolation of antibody from a synthetic human antibody library by repeated fluorescence-activated cell sorting (FACS)An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains.Functional enrichment by direct plasmid recovery after fluorescence activated cell sorting.Antibody affinity maturation through combining display of two-chain paired antibody and precision flow cytometric sorting.BrkAutoDisplay: functional display of multiple exogenous proteins on the surface of Escherichia coli by using BrkA autotransporter.
P2860
Q27654632-A84EB0D2-9E21-4826-879B-90D39960674CQ28275218-A169FD45-FCEE-4FE9-9C21-D57DF3BB6538Q28295016-CFCBAEC6-01B3-4ACE-A4BB-98605C3E76D1Q28534600-465D40E3-098C-4A07-880A-15C0C497D2D5Q28914723-753F9586-8E95-4BA9-A4DA-BE8FB70868ADQ30155216-05DA7270-01B1-4740-8FE1-7906737314F4Q30377057-9665A931-3C1F-4A3A-81C6-A3154C87596DQ33248009-15668862-7959-4108-9E96-1A25CEB105E1Q33274569-B224AE1D-B20C-46F6-98CB-E913C4631716Q33282948-0CB15591-21B5-4059-8E93-B680DCA98245Q33283622-75B31C23-7976-4F19-AF5F-A8A4EBAA525CQ33284325-89C481FE-87B4-4FB0-BD8D-B42648BE1AAFQ33285349-2227A773-3E3B-4160-9B75-509E81210064Q33379067-9C95371B-B7D1-4D94-B379-962BAE83FB52Q33403805-7D885DD4-326E-46A4-B1B8-957842F3C435Q33483445-FB52B90C-F8BA-4EAC-835D-3F474B3CADBEQ33505005-A609C40A-CBF4-45DC-82E3-A02B2145BDAEQ33519833-33B12FE5-58CF-48A4-9C1A-D9DF82A488F7Q33564662-46F6DC03-61B1-4244-91B5-C90280B37CF8Q33568256-CD074604-A297-4407-BC29-1B394FD1F7B3Q33970172-D6C79B40-78A5-433B-BF4A-E775BACA4577Q34047293-BBCBE6EF-08FD-45A1-9046-E362A42D170CQ34111656-8AE9C5F4-CD2C-4E98-AC8C-0218BB8D0A81Q34124085-815E47E5-A0A4-4E62-AD8D-A9151C7BC027Q34194728-6747E2EB-590E-4B60-AA21-F8F2A9F31609Q34419541-F299FA8E-7FDF-44BF-9D90-20E3AAE6DDBCQ34445095-8FF6A1C9-6BA4-4289-B1C3-4D28B93A66E6Q34476404-267EE431-73BE-430E-B8E5-A2F7A2F194BDQ34578048-0EF3AB8D-5EA5-42D0-90C5-6FECB19B2A2EQ34697519-97FA6793-99F3-4656-A4AD-1F070D10BD2EQ34720183-F2431015-394E-4F66-8E62-A1C4D2D482AAQ35005394-3F8188E5-0CC8-4D45-BA03-6A0F1E7EA8F1Q35080183-7C46369E-0520-49F7-ABD9-D3A3505000C9Q35101020-11BEBBD6-5B3B-4AF1-AFE5-28FA44765F35Q35216785-66D8098E-E9CA-407B-9C2B-CD16FD0F2162Q35320978-B0BB0749-C2D5-48E4-AB55-43B5053F3A5CQ35535298-2279C39B-6771-4FF7-B697-91843AE1AA79Q35765870-7BF84B74-D1D0-4655-9B5C-885865895CA5Q36007852-23314D2C-893B-45A7-942B-6ECB78CDE1C8Q36022993-58190FFE-A6CE-42E5-AEB3-26C8000349B4
P2860
Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries.
description
2004 nî lūn-bûn
@nan
2004 թուականի Յունիսին հրատարակուած գիտական յօդուած
@hyw
2004 թվականի հունիսին հրատարակված գիտական հոդված
@hy
2004年の論文
@ja
2004年論文
@yue
2004年論文
@zh-hant
2004年論文
@zh-hk
2004年論文
@zh-mo
2004年論文
@zh-tw
2004年论文
@wuu
name
Anchored periplasmic expressio ...... chia coli-expressed libraries.
@ast
Anchored periplasmic expressio ...... chia coli-expressed libraries.
@en
type
label
Anchored periplasmic expressio ...... chia coli-expressed libraries.
@ast
Anchored periplasmic expressio ...... chia coli-expressed libraries.
@en
prefLabel
Anchored periplasmic expressio ...... chia coli-expressed libraries.
@ast
Anchored periplasmic expressio ...... chia coli-expressed libraries.
@en
P2093
P2860
P356
P1476
Anchored periplasmic expressio ...... chia coli-expressed libraries.
@en
P2093
Andrew Hayhurst
Barrett R Harvey
Brent L Iverson
Geoffrey K Rogers
George Georgiou
Ki Jun Jeong
P2860
P304
P356
10.1073/PNAS.0400187101
P407
P577
2004-06-14T00:00:00Z