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Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperaturesEngineering an enhanced, thermostable, monomeric bacterial luciferase gene as a reporter in plant protoplastsToward Computationally Designed Self-Reporting Biosensors Using Leave-One-Out Green Fluorescent ProteinDesigning specific protein-protein interactions using computation, experimental library screening, or integrated methodsChemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.Identifying functionally important mutations from phenotypically diverse sequence data.Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluoresceinIdentification of novel synthetic toll-like receptor 2 agonists by high throughput screening.Processing DNA molecules as textRole of the loop L4,5 in allosteric regulation in mtHsp70s: in vivo significance of domain communication and its implications in protein translocationSelection of drug resistant mutants from random library of Plasmodium falciparum dihydrofolate reductase in Plasmodium berghei modelAn experimentally determined evolutionary model dramatically improves phylogenetic fit.Engineering of Clostridium phytofermentans Endoglucanase Cel5A for improved thermostability.Restriction enzyme-free construction of random gene mutagenesis libraries in Escherichia coli.An automated flow for directed evolution based on detection of promiscuous scaffolds using spatial and electrostatic properties of catalytic residues.Molecular insights revealing interaction of Tim23 and channel subunits of presequence translocaseA measure of the broad substrate specificity of enzymes based on 'duplicate' catalytic residuesA novel C-terminal region within the multicargo type III secretion chaperone CesT contributes to effector secretion.Directed evolution of the substrate specificity of dialkylglycine decarboxylaseLessons from diversity of directed evolution experiments by an analysis of 3,000 mutations.A method for multi-codon scanning mutagenesis of proteins based on asymmetric transposonsSchizosaccharomyces pombe Ccq1 and TER1 bind the 14-3-3-like domain of Est1, which promotes and stabilizes telomerase-telomere association.Error-prone pcr-based mutagenesis strategy for rapidly generating high-yield influenza vaccine candidates.Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.Engineering better biomass-degrading ability into a GH11 xylanase using a directed evolution strategy.Directed Evolution of Enzymes for Industrial Biocatalysis.Characterization of a cold-active esterase from Serratia sp. and improvement of thermostability by directed evolutionMassively parallel single-nucleotide mutagenesis using reversibly terminated inosine.A Simple Combinatorial Codon Mutagenesis Method for Targeted Protein EngineeringAn engineered cryptic Hxt11 sugar transporter facilitates glucose-xylose co-consumption in Saccharomyces cerevisiaeBeyond Antibodies as Binding Partners: The Role of Antibody Mimetics in Bioanalysis.Perspective on Adeno-Associated Virus Capsid Modification for Duchenne Muscular Dystrophy Gene TherapyComprehensive cloning of patient-derived 9022-bp amplicons of hepatitis C virus.Directed polymerase evolution.Deep sequencing methods for protein engineering and design.Mapping of a microbial protein domain involved in binding and activation of the TLR2/TLR1 heterodimer.Directed evolution of Vibrio fischeri LuxR for improved response to butanoyl-homoserine lactone.Low-fidelity Pyrococcus furiosus DNA polymerase mutants useful in error-prone PCR.Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes.Charting the signal trajectory in a light-oxygen-voltage photoreceptor by random mutagenesis and covariance analysis
P2860
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P2860
description
2003 nî lūn-bûn
@nan
2003 թուականի Յունուարին հրատարակուած գիտական յօդուած
@hyw
2003 թվականի հունվարին հրատարակված գիտական հոդված
@hy
2003年の論文
@ja
2003年論文
@yue
2003年論文
@zh-hant
2003年論文
@zh-hk
2003年論文
@zh-mo
2003年論文
@zh-tw
2003年论文
@wuu
name
Generating mutant libraries using error-prone PCR.
@ast
Generating mutant libraries using error-prone PCR.
@en
type
label
Generating mutant libraries using error-prone PCR.
@ast
Generating mutant libraries using error-prone PCR.
@en
prefLabel
Generating mutant libraries using error-prone PCR.
@ast
Generating mutant libraries using error-prone PCR.
@en
P2093
P356
P1476
Generating mutant libraries using error-prone PCR.
@en
P2093
Daisuke Umeno
Kimberly M Mayer
Patrick C Cirino
P356
10.1385/1-59259-395-X:3
P407
P577
2003-01-01T00:00:00Z