Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein

Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein

Item
http://www.wikidata.org/entity/Q33216583
A streptavidin variant with slower biotin dissociation and increased mechanostabilityA monovalent streptavidin with a single femtomolar biotin binding siteHow the biotin–streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramerEvolved streptavidin mutants reveal key role of loop residue in high-affinity bindingStreptavidin and its biotin complex at atomic resolutionStructure-based engineering of streptavidin monomer with a reduced biotin dissociation rateBifunctional avidin with covalently modifiable ligand binding site.Construction of chimeric dual-chain avidin by tandem fusion of the related avidins.Single M13 bacteriophage tethering and stretchingRapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis.Functional characterization of ent-copalyl diphosphate synthase, kaurene synthase and kaurene oxidase in the Salvia miltiorrhiza gibberellin biosynthetic pathwayDirected evolution of streptavidin variants using in vitro compartmentalization.Efficient delivery of p53 and cytochrome c by supramolecular assembly of a dendritic multi-domain delivery system.Experimental evolution of a green fluorescent protein composed of 19 unique amino acids without tryptophan.A novel, dual cytokine-secretion assay for the purification of human Th22 cells that do not co-produce IL-17A.
P2860

Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein

Item
http://www.wikidata.org/entity/Q33216583
description
2005 nî lūn-bûn
@nan
2005 թուականի Յունիսին հրատարակուած գիտական յօդուած
@hyw
2005 թվականի հունիսին հրատարակված գիտական հոդված
@hy
2005年の論文
@ja
2005年論文
@yue
2005年論文
@zh-hant
2005年論文
@zh-hk
2005年論文
@zh-mo
2005年論文
@zh-tw
2005年论文
@wuu
name
Engineered single-chain dimeri ...... rence for biotin-4-fluorescein
@ast
Engineered single-chain dimeri ...... rence for biotin-4-fluorescein
@en
type
label
Engineered single-chain dimeri ...... rence for biotin-4-fluorescein
@ast
Engineered single-chain dimeri ...... rence for biotin-4-fluorescein
@en
prefLabel
Engineered single-chain dimeri ...... rence for biotin-4-fluorescein
@ast
Engineered single-chain dimeri ...... rence for biotin-4-fluorescein
@en
P1433
P1476
P2093
P2860
P304
P31
P356
P407
P433
P478
P577
P5875
P698
P932
P356
P1433
P1476
Engineered single-chain dimeri ...... rence for biotin-4-fluorescein
@en
P2093
P2860
P304
P31
P356
P407
P433
P478
P577
P5875
P698
P932